American Journal of Food Science and Technology
ISSN (Print): 2333-4827 ISSN (Online): 2333-4835 Website: Editor-in-chief: Hyo Choi
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American Journal of Food Science and Technology. 2015, 3(3), 67-73
DOI: 10.12691/ajfst-3-3-3
Open AccessArticle

Combining Different Types of Prebiotic Plant Isolates Toward, Enhancing the Growth of Probiotic Organisms

Fathima Hajara Aslam1, , Jagath Wansapala2 and Manel Perera3

1Department of Food Science, Spectrum Institute of Science and Technology, Colombo, Sri-Lanka

2Department of Food Science and Technology, Faculty of Applied Sciences, University of Sri-Jayewardenepura, Gangodawila, Nugegoda, Sri-Lanka

3MicroChem Laboratories, Nugegoda, Sri-Lanka

Pub. Date: June 16, 2015

Cite this paper:
Fathima Hajara Aslam, Jagath Wansapala and Manel Perera. Combining Different Types of Prebiotic Plant Isolates Toward, Enhancing the Growth of Probiotic Organisms. American Journal of Food Science and Technology. 2015; 3(3):67-73. doi: 10.12691/ajfst-3-3-3


The growth stimulatory effect produced by combining different sources of prebiotics i.e; Fiber isolates from Musa sp pseudostem, polyphenol extracts from Sesbania grandiflora flower petal and non-digestible polysaccharide extracts from Artocarpus heterophyllus seed were assessed against probiotic organisms, Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis BB-12 in vitro in liquid cultures. Different combinations were formulated by integrating the three sources of prebiotics at two different levels i.e; fibre (0.2% and 2%), polyphenol extracts (0.2% and 0.6%) and non-digestible polysaccharide extracts (0.2% and 1.2%) to obtain eight treatments. The formulation which consisted 2% fibre, 0.2% polyphenol and 0.2% non-digestible polysaccharide was able to promote significant biomass increment in Lactobacillus acidophilus, while the treatment consisting 2% fibre, 0.6% polyphenol and 0.2% non-digestible polysaccharide demonstrated highest proliferation for Bifidobacterium animalis subsp. lactis BB-12 in vitro which were statistically different (p<0.05) than other formulations.

L. acidophilus B. animalis subsp. lactis BB-12 S. grandiflora Musa sp A. heterophyllus

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[1]  Ogueke, C.C., et al., probiotics and prebiotics Parkistan journal of nutrition, 2010. 9: p. 833-843.
[2]  Pyar, H. and K.K. Peh, Characterization and IdentIfication of Lactobacillus acidophilus using Biolog Rapid Identification System. International Journal of Pharmacy and Pharmaceutical Sciences, 2014. Vol 6(Issue 1).
[3]  Gorbach, S.L., Lactic acid bacteria and human health. Ann Med, 1990. 22(1): p. 37-41.
[4]  Servin, A.L., Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens. FEMS Microbiology Reviews, 2004. 28 p. 405–440.
[5]  Rossi, M. and A. Amaretti, Probiotic properties of bifidobacteria. 2010: Caister Academic Press: Norfolk, UK.
[6]  Ishibashi, N., T. Yaeshima, and H. Hayasawa, Bifidobacteria: their significance in human intestinal health. Mal J Nutr, 1997. 3: p. 149-159.
[7]  Mohd Shaufi, M.A., et al., Deciphering chicken gut microbial dynamics based on high-throughput 16S rRNA metagenomics analyses. Gut Pathog, 2015. 7(4): p. 015-0051.
[8]  Fuller, R. and G. Peridigón, Gut flora, nutrition, immunity and health. 2008: John Wiley & Sons.
[9]  Sousa, V.M.C., E.F. Santos, and V.C. Sgarbieri, The Importance of Prebiotics in Functional Foods and Clinical Practice. Food and Nutrition Sciences, 2011. Vol. 2 p. 4.
[10]  Huebner, J., Effect of processing conditions on the prebiotic activity of commercial prebiotics. International Dairy Journal, 2008. 18: p. 287-293.
[11]  Gibson, G.R., Dietary modulation of the human gut microflora using prebiotics. The British Journal of Nutrition, 1998. 4.
[12]  Grajek, W., Probiotics, prebiotics and antioxidants as functional foods. on-line at:, 2005. 52: p. 665-671.
[13]  Munde-Wagh, K.B., Phytochemical, Antimicrobial Evaluation and Determination of Total Phenolic and Flavonoid Contents of Sesbania grandiflora flower extracts International Journal of Pharmacy and Pharmaceutical Sciences 2012. 4(4).
[14]  China, R., et al., Antimicrobial activity of Sesbania grandiflora flower polyphenol extracts on some pathogenic bacteria and growth stimulatory effect on the probiotic organism Lactobacillus acidophilus. Microbiological Research, 2012. 167: p. 500-506.
[15]  Cardona, F., Benefits of polyphenols on gut microbiota and implications in human health. The Journal of Nutritional Biochemistry, 2013. 24(8): p. 1415-1422.
[16]  Mukhopadhyay, S., et al., Banana fibers–variability and fracture behaviour. Cellulose, 2008. 31(3.61).
[17]  Thammarutwasik, P., et al., Prebiotics – A Review. Songklanakarin J. Sci. Technol, 2009. 31: p. 401-408.
[18]  Arthanarieswaran, V.P., A. Kumaravel, and M. Kathirselvam, Evaluation of mechanical properties of banana and sisal fiber reinforced epoxy composites: Influence of glass fiber hybridization. Materials & Design, 2014. 64: p. 194-202.
[19]  China, R., et al., In vitro antioxidant activity of different cultivars of banana flower (Musa paradicicus L.) extracts available in India. J Food Sci 2011. 76(9): p. 1292-9.
[20]  Veeraphong Bhornsmithikun, P.C., Ram Yamsaengsung, and a.K. Prasertsit, Continuous extraction of prebiotics from jackfruit seeds. Songklanakarin J. Sci. Technol, 2010. 6: p. 635-642.
[21]  Bzducha, A. and M.W. Obiedziński, Influence of two probiotic Lactobacillus strains on CLA content in model ripening cheese polish journal of food and nutrition sciences, 2007. 57: p. 65-69.
[22]  Shadan Abas, A.-W.A.A.A.-S., Screening for Bacteriocins Production in Enteric Bifidobacterium Isolates and Study of Some Production Affecting Factors. Medical Journal of Babylon, 2012. 9.
[23]  Singleton, V.L., Orthofer, R. and Lamuela-Raventos, R.M, Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocaltue reagent. MethEnzymol, 1999. 299: p. 152-178.
[24]  Buttriss, J.L. and C.S. Stokes, Dietary fibre and health: an overview. Nutrition Bulletin, 2008. 33(3): p. 186-200.
[25]  Aziz, N.A.A., Chemical and functional properties of the native banana (Musa acuminata × balbisiana Colla cv. Awak) pseudo-stem and pseudo-stem tender core flours. Food Chemistry, 2011. 128(3): p. 748-753.
[26]  Slavin, J., Fiber and Prebiotics: Mechanisms and Health Benefits. Nutrients, 2013. 5(4): p. 1417-1435.
[27]  Farooq, U., et al., Enhancement of short chain fatty acid production from millet fibres by pure cultures of probiotic fermentation. Tropical Journal of Pharmaceutical Research, 2013. 12(2): p. 189-194.
[28]  Wang, J., et al., In vitro fermentation of xylooligosaccharides from wheat bran insoluble dietary fiber by Bifidobacteria. Carbohydrate polymers, 2010. 82(2): p. 419-423.
[29]  Kemperman, R.A., C. Bolca, and L.C.a.V. Roger, E.E, Novel approaches for analysing gut microbes and dietary polyphenols: challenges and opportunities. Microbiology, 2010. 156: p. 3224–3231.
[30]  Manach, C., et al., Bioavailability of rutin and quercetin in rats FEBS Letters, 1997. 409: p. 12-16.
[31]  Parkar, S.G., T.M. Trower, and D.E. Stevenson, Fecal microbial metabolism of polyphenols and its effects on human gut microbiota. Anaerobe 2013. 23: p. 12-19.
[32]  Wichienchot, S., et al., Extraction and analysis of prebiotics from selected plants from southern Thailand. Songklanakarin J. Sci. Technol, 2011. 33(5): p. 517-523.
[33]  Idrees, M., et al., Enzymatic saccharification and lactic acid production from banana pseudo-stem through optimized pretreatment at lowest catalyst concentration. EXCLI Journal, 2013. 12: p. 269-281.
[34]  Montet, D., R.C. Ray, and N. Zakhia-Rozis, Lactic Acid Fermentation of Vegetables and Fruits. Microorganisms and Fermentation of Traditional Foods, 2014: p. 108.