Journal of Food and Nutrition Research
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Journal of Food and Nutrition Research. 2017, 5(8), 594-605
DOI: 10.12691/jfnr-5-8-10
Open AccessArticle

Comparison of Anti-obesity Effects of Spirit Vinegar and Natural Fermented Vinegar Products on the Differentiation of 3T3-L1 Cells and Obese Rats Fed a High-fat Diet

Hee-Kyoung Son1, Yeon-Kyoung Kim1, Hye Won Shin2, Hee Jeong Lim2, Byoung-Seok Moon2 and Jae-Joon Lee1,

1Department of Food and Nutrition, Chosun University, Gwangju, Korea

2Foods Research Institute, CJ Cheil Jedang Corp., Suwon, Korea

Pub. Date: July 29, 2017

Cite this paper:
Hee-Kyoung Son, Yeon-Kyoung Kim, Hye Won Shin, Hee Jeong Lim, Byoung-Seok Moon and Jae-Joon Lee. Comparison of Anti-obesity Effects of Spirit Vinegar and Natural Fermented Vinegar Products on the Differentiation of 3T3-L1 Cells and Obese Rats Fed a High-fat Diet. Journal of Food and Nutrition Research. 2017; 5(8):594-605. doi: 10.12691/jfnr-5-8-10


This study aimed to compare the effects of spirit vinegar and four types of natural fermented vinegar products (apple vinegar, brown rice vinegar, lemon vinegar, and balsamic vinegar) on the differentiation of 3T3-L1 cells and obesity in rats fed a high-fat diet (HFD). Vinegar inhibited the accumulation of lipid droplets during the differentiation of 3T3-L1 cells. Body weight gains, visceral fat pad weights, serum leptin and triglyceride levels, and hepatic lipogenic enzyme mRNA levels were significantly decreased in the HF-vinegar administered groups compared with those in the HF group. Furthermore, oral administration of vinegars significantly reduced the occurrence of fatty liver deposits and steatosis, and inhibited the HF diet-induced increase in adipocyte size. The anti-obesity and lipid-lowering effects were slightly greater in the HF-natural fermented vinegar groups than in the HF-spirit vinegar group. Apple vinegar had the strongest anti-obesity effects in 3T3-L1 cells and obese rats compared with the other tested vinegars. These findings indicated that vinegar administration may have potential for improving some obesity-related parameters in 3T3-L1 cells and obese rats.

natural fermented vinegar spirit vinegar 3T3-L1 cells differentiation high-fat diet anti-obesity

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[1]  Leung WY, Thomas GN, Chan JC, Tomlinson B. “Weight management and current options in pharmacotherapy: orlistat and sibutramine”, Clinical Therapeutics, 25(1), 58-80, 2003.
[2]  Wang YW, Jones PJ. “Conjugated linoleic acid and obesity control: efficacy and mechanisms”, International Journal of Obesity, 28(8), 941-955, 2004.
[3]  George AB. Louis AT. “Medicinal strategies in the treatment of obesity”, Nature, 404(6778), 672-677, 2000.
[4]  Guerrero EDA, Mejias RC, Marin RN, Lovillo MP, Barroso CG. “A new FT-IR method combined with multivariate analysis for the classification of vinegars from different raw materials and production processes”, Journal of Science Food and Agriculture, 90(4), 721-728, 2010.
[5]  Ho CW, Lazim AM, Fazry S, Zaki UKHH, Lim SJ. “Varieties, production, composition and health benefits of vinegars: A review,” Food Chemistry, 221(2017), 1621-1630, 2017.
[6]  Lim EJ, Cha GH. “Study on manufacturing of vinegar through literatures of the Joseon dynasty”, Journal of the Korean Society of Food Culture, 25(6), 680-707, 2010.
[7]  Korea Food Standard Code (KFDA). “Korea Food and Drug Administration”, Cheongwon, Korea, pp5-21, 2012.
[8]  Andrés-Barrao C, Saad MM, Cabello Ferrete E, Bravo D, Chappuis ML, Ortega Pérez R, Junier P, Perret X, Barja F. “Metaproteomics and ultrastructure characterization of Komagataeibacter spp. involved in high-acid spirit vinegar production”, Food Microbiology, 55(2016), 112-122, 2016.
[9]  Sinclair C. “International Dictionary of Food and Cooking”, 1st Edn, Fitzroy Dearborn Publishers, Chicago∙London, UK, pp3-5, 1998.
[10]  Shimoji Y, Tamura Y, Nakamura Y, Nanda K, Nishidai S, Nishikawa Y, Tanaka T. “Isolation and identification of DPPH radical scavenging compounds in Kurosu (Japanese unpolished rice vinegar)”, Journal of Agricultural and Food Chemistry, 50(20), 6501-6503, 2002.
[11]  Alonso AM, Castro R, Rodrıguez MC, Guillen DA, Barroso CG. “Study of the antioxidant power of brandies and vinegars derived from sherry wines and correlation with their content in polyphenols”, Food Research International, 37(7), 715-721, 2004.
[12]  Jeong CH, Choi GN, Kim JH, Kawk JH, Kang ST, Choi SG, Heo HJ. “In vitro antioxidant properties and phenolic composition of Korean commercial vinegars”, Food Science and Biotechnology, 18(5), 1258-1262, 2009.
[13]  Gonzalez-Molina E, Dominguez-Perles R, Moreno DA, Garcia-Viguera C. “Natural bioactive compounds of Citrus limon for food and health”, Journal of Pharmaceutical and Biomedical Analysis, 51(2), 327-345, 2010.
[14]  Moon YJ, Choi DS, Oh SH, Song YS, Cha YS. “Effects of persimmon-vinegar on lipid and carnitine profiles in mice”, Food Science and Biotechnology, 19(2), 343-348, 2010.
[15]  Chou CH, Liu CW, Yang DJ, Wu YH, Chen YC. “Amino acid, mineral, and polyphenolic profiles of black vinegar, and its lipid lowering and antioxidant effects in vivo”, Food Chemistry, 168(1), 63-66, 2015.
[16]  Vogel RA, Correti MC, Plotnick GD. “The postprandial effect of components of the Mediterranean diet on endothelial function”, Journal of the American College of Cardiology, 36(5), 1455-460, 2000.
[17]  Yamashita H, Fujisawa K, Ito E, Idei S, Kawaguchi N, Kimoto M, Hiemori M, Tsuji H. “Improvement of obesity and glucose tolerance by acetate in type 2 diabetic Otsuka Long-Evans Tokushima fatty (OLETF) rats”, Bioscience Biotechnology, and Biochemstry, 71(5), 1236-1243, 2007.
[18]  Johnston CS, Quagliano S, White S. “Vinegar ingestion at mealtime reduced fasting blood glucose concentrations in healthy adults at risk for type 2 diabetes”, Journal of Functional Foods, 5(4), 2007-2011, 2013.
[19]  Kondo T, Kishi M, Fushimi T, Kaga T. “Acetic acid upregulates the expression of genes for fatty acid oxidation enzymes in liver to suppress body fat accumulation”, Journal of Agricultural and Food Chemistry, 57(13), 5982-5986, 2009.
[20]  Kondo T, Kishi M, Fushimi T, Ugajin S, Kaga T. “Vinegar intake reduces body weight, body fat mass, and serum triglyceride levels in obese Japanese subjects”, Bioscience Biotechnology, and Biochemstry, 73(8), 1837-1843, 2009.
[21]  Lee JH, Cho HD, Jeong JH, Lee MK, Jeong YK, Shim KW, Seo KI. “New vinegar produced by tomato suppresses adipocyte differentiation and fat accumulation in 3T3-L1 cells and obese rat model”, Food Chemistry, 141(3), 3241-3249, 2013.
[22]  Park YH, Choi JH, Whang K, Lee SO, Yang SA, Yu MH. “Inhibitory effects of lyophilized dropwort vinegar powder on adipocyte differentiation and inflammation”, Journal of Life Science, 24(5), 476-484, 2014.
[23]  Kondo S, Tayama K, Tsukamoto Y, Ikeda K, Yamori Y. “Antihypertensive effects of acetic acid and vinegar on spontaneously hypertensive rats”, Bioscience Biotechnology, and Biochemstry, 65(12), 2690-2694, 2001.
[24]  Kim H, Lee H, Shin KS. “Intestinal immunostimulatory activity of neutral polysaccharide isolated from traditionally fermented Korean brown rice vinegar”, Bioscience Biotechnology, and Biochemstry, 80(12), 2383-2390, 2016.
[25]  Nakao C, Yamada E, Fukaya M, Tayama K, Tsukamoto Y, Sato Y. “Effect of acetate on glycogen replenishment in liver and skeletal muscles after exhaustive swimming in rats”, Scandinavian Journal of Medicine & Science in Sports, 11(1), 33-37, 2001.
[26]  Fushimi T, Suruga K, Oshima Y, Fukiharu M, Tsukamoto Y, Goda T. “Dietary acetic acid reduces serum cholesterol and triacylglycerols in rats fed a cholesterol-rich diet”, British Journal of Nutrition, 95(5), 916-924, 2006.
[27]  Tong LT, Katakura Y, Kawamura S, Baba S, Tanaka Y, Udono M, Kondo Y, Nakamura K, Imaizumi K, Sato M. “Effects of Kurozu concentrated liquid on adipocyte size in rats”, Lipids in Health and Disease, 9, 134-146, 2010.
[28]  Sakanaka S, Ishihara Y. “Comparison of antioxidant properties of persimmon vinegar and some other commercial vinegars in radical-scavenging assays and on lipid oxidation in tuna homogenates”, Food Chemistry, 107(2), 739-744, 2008.
[29]  Masinoa F, Chinnici F, Bendini A, Montevecchi G, Antonelli A. “A study on relationships among chemical, physical, and qualitative assessment in traditional balsamic vinegar”, Food Chemistry, 106(1), 90-95, 2008.
[30]  Cho AS, Jeon SM, Kim MJ, Yeo J, Seo KI, Choi MS, Lee MK. “Chlorogenic acid exhibits anti-obesity property and improves lipid metabolism in high-fat diet-induced-obese mice”, Food and Chemical Toxicology, 48(3), 937-943, 2010.
[31]  Snyder SM, Zhao B, Luo T, Kaiser C, Cavender G, Hamilton-Reeves J, Sullivan DK, Shay NF. “Consumption of quercetin and quercetin-containing apple and cherry extracts affects blood glucose concentration, hepatic metabolism, and gene expression patterns in obese C57BL/6J high fat-fed mice”, Journal of Nutrition, 146(5), 1001-1007, 2016.
[32]  Brimson JM, Brimson SJ, Brimson CA, Rakkhitawatthana V, Tencomnao T. “Rhinacanthus nasutus extracts prevent glutamate and amyloid-β neurotoxicity in HT-22 mouse hippocampal cells: possible active compounds include lupeol, stigmasterol and β-sitosterol”, International Journal of Molecular Sciences, 13(4), 5074-5097, 2012.
[33]  Reeves PG, Nielson FH, Fahey Jr GC. “AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition adhoc writing committee on the reformulation of the AIN-76A rodent diet”, Journal of Nutrition, 123(11), 1939-1951, 1993.
[34]  Friedwald W, Levy R, Fredrickson D. “Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge”, Clinical Chemistry, 18(6), 499-502, 1972.
[35]  Folch J, Lees M, Sloane-Stanley G. “A simple method for the isolation and purification of total lipids from animal tissues”, Journal of Biological Chemistry, 226(1), 497-509, 1957.
[36]  Biggs HG, Erikson TM, Moorehead WR. “A manual colorimetric assay of triglyceride in serum”, Clinical Chemistry, 21(3), 437-441, 1975.
[37]  Zlatkis A, Zak B. “Study of a new cholesterol reagent”, Analytical Biochemistry, 29(1), 43-48, 1969.
[38]  Nakabayashi H, Hashimoto T, Ashida H, Nishiumi S, Kanazawa K. “Inhibitory effects of caffeine and its metabolites on intracellular lipid accumulation in murine 3T3-L1 adipocytes”, Biofactors, 34(4), 293-302, 2008.
[39]  Karamaĉ M, Amarowicz R. “Inhibition of pancreatic lipase by phenolic acids: examination in vitro”, Zeitschrift fuer Naturforschung Section C Journal of Biosciences, 51(11-12), 903-905, 1996.
[40]  Ok E. Do GM, Lim Y, Park JE, Park YJ, Kwon O. “Pomegranate vinegar attenuates adiposity in obese rats through coordinated control of AMPK signaling in the liver and adipose tissue”, Lipids in Health and Disease, 12, 163-171, 2013.
[41]  Nakajima VM, Macedo GA, Macedo JA. “Citrus bioactive phenolics: Role in the obesity treatment”, LWT – Food Science and Technology, 59(2), 1205-1212, 2014.
[42]  Wang S, Moustaid-Moussa N, Chen L, Mo H, Shastri A, Su R, Bapat P, Kwun I, Shen CL. “Novel insights of dietary polyphenols and obesity”, The Journal of Nutritional Biochemistry, 25(1), 1-18, 2014.
[43]  Etherton PMK, Lefevre M, Beecher GR, Gross MD, Keen CL, Etherton TD. “Bioactive compounds in nutrition and health-research methologies for establishing biological function: the antioxidant and anti-inflammatory effects of flavonoids on the atherosclerosis”, Annual Review of Nutrition, 24, 511-538, 2004.
[44]  Hsu CL, Yen GC. “Effects of flavonoids and phenolic acids on the inhibition of adipogenesis in 3T3-L1 adipocytes”, Journal of Agricultural and Food Chemistry, 55(21), 8404-8410, 2007.
[45]  Noratto GN, Martino HS, Simbo S, Byrne D, Mertens-Talcott SU. “Consumption of polyphenol-rich peach and plum juice prevents risk factors for obesity-related metabolic disorders and cardiovascular disease in Zucker rats”, Journal of Nutritional Biochemistry, 26(6), 633-641, 2015.
[46]  Fukuchi Y, Hiramitsu M, Okada M, Hayashi S, Nabeno Y, Osawa T, Naito M. “Lemon polyphenols suppress diet-induced obesity by up-regulation of mRNA levels of the enzymes involved in beta-oxidation in mouse white adipose tissue”, Journal of Clinical Biochemistry and Nutrition, 43(3), 201-209, 2008.
[47]  Ramirez-Zacarias J, Castro-Munozledo F, Kuri-Harcuch W. “Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with oil red O”, Histochemistry, 97(6), 493-497, 1992.
[48]  Ho JN, Son ME, Lim WC, Lim ST, Cho HY. “Antiobesity effects of germinated brown rice extract through down-regulation of lipogenesis genes in high fat diet-induced obese mice”, Bioscience Biotechnology, and Biochemistry, 76(6), 1068-1074, 2012.
[49]  Hamadate K, Nakamura K, Hirai M, Yamamoto T, Yamaguchi H, Iizuka M, Yamamoto E, Iwama Y, Yazawa K. “Effect of a dietary supplement containing Kurozu (a Japanese traditional health drink) concentrate on several obesity-related parameters in obese Japanese adults: a randomized, double-blind, placebo-controlled trial”, Functional Foods in Health & Disease, 3(8), 310-322, 2013.
[50]  Bjorntorp P. “The associations between obesity, adipose tissue distribution and disease”, Journal of Internal Medicine, 723, 121-134, 1998.
[51]  Friedman JM, Halaas JL. “Leptin and the regulation of body weight in mammals”, Nature, 395(6704), 763-770, 1998.
[52]  Havel PJ. “Role of adipose tissue in body-weight regulation: mechanism regulating leptin production and energy balance”, Proceedings of the Nutrition Society, 59(3), 359-371, 2000.
[53]  Morton NM, Paterson JM, Masuzaki H, Holmes MC, Staels B, Fievet C, Walker BR, Flier JS, Mullins JJ, Seckl JR. “Novel adipose tissue-mediated resistance to diet-induced visceral obesity in 11-beta-hydroxysteroid dehydrogenase type 1-deficient mice”, Diabetes. 53(4), 931-938, 2004.
[54]  Laranjinha JA, Almeida LM, and Madeira VM. “Reactivity of dietary phenolic acids with peroxyl radicals: antioxidant activity upon low density lipoprotein peroxidation”, Biochemical Pharmacology, 48(3), 487-494, 1994.
[55]  Budak NH, Kumbul Doguc D, Savas CM, Seydim AC, Kok Tas T, Ciris MI, Guzel-Seydim ZB. “Effects of apple cider vinegars produced with different techniques on blood lipids in high-cholesterol-fed rats”, Journal of Agricultural and Food Chemistry, 59(12), 6638-6644, 2011.
[56]  Browning JD, Horton JD. “Molecular mediators of hepatic steatosis and liver injury”, The Journal of Clinical Investigation, 114(2), 147-152, 2004.
[57]  Schaefer EJ. “Lipoproteins, nutrition, aging, and atherosclerosis”, The American Journal of Clincal Nutrition, 61(3), 726S-740S, 1995.
[58]  Buettner R, Parhofer KG, Woenckhaus M, Wrede CE, Kunz-Schughart LA, Scholmerich J, Bollheimer LC. “Defining high-fat-diet rat models: metabolic and molecular effects of different fat types”, Journal of Molecular Endocrinology, 36(3), 485-501, 2006.
[59]  Mohamad NE, Yeap SK, Lim KL, Yusof HM, Beh BK, Tan SW, Ho WY, Sharifuddin SA, Jamaluddin A, Long K, Nik Abd Rahman NM, Alitheen NB. “Antioxidant effects of pineapple vinegar in reversing of paracetamol-induced liver damage in mice”, Chinese Medicine, 10, 3-13, 2015.
[60]  Bounihi A, Bitam A, BouazzaA, Yargui L, Koceir EA. “Fruit vinegars attenuate cardiac injury via anti-inflammatory and anti-adiposity actions in high-fat diet-induced obese rats”, Pharmaceutical Biology, 55(1), 43-52, 2017.