American Journal of Food Science and Technology
ISSN (Print): 2333-4827 ISSN (Online): 2333-4835 Website: Editor-in-chief: Hyo Choi
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American Journal of Food Science and Technology. 2014, 2(5), 145-149
DOI: 10.12691/ajfst-2-5-2
Open AccessArticle

Microbiological and Sensory Profile of Soymilk Based Juice Treated with Liquid Extract of A. Danielli

Dauda A.O1 and Adegoke G.O.1,

1Department of Food Technology, University of Ibadan, Ibadan, Oyo State, Nigeria, West Africa

Pub. Date: October 08, 2014

Cite this paper:
Dauda A.O and Adegoke G.O.. Microbiological and Sensory Profile of Soymilk Based Juice Treated with Liquid Extract of A. Danielli. American Journal of Food Science and Technology. 2014; 2(5):145-149. doi: 10.12691/ajfst-2-5-2


The microbiological and sensory profile soymilk-based juice treated with aqueous extract of Aframomum danielli (1%-3%-w/v) and stored at 27±2C for twenty four weeks were examined. Synthetic additives/preservatives, which have been in use over the years, are more expensive and mostly imported with hard earned scarce foreign exchange. Adequate research work into the production and utilization of indigenous food additives on a large scale has not been undertaken in developing countries. This work was therefore planned to ascertain the usefulness of extracts of A. danielli, a local spice, in stabilizing the microbiological quality of soymilk-based juice. Standard methods were used for the production of juice samples from pineapple, orange, carrot and milk from soybeans. They were blended together in equal proportion and thereafter treated with A. danielli extract (1.0% to 3.0%) and stored at 27+2C for twenty four weeks. Infrared spectrophotometer was used to identify functional groups in Aframomum danielli and hence the active components responsible for the stability of the juice. Standard AOAC method was used for microbiological analysis of treated and untreated samples. Consumer evaluation of juice samples was done using analysis of variance (ANOVA). Some of the active components of the spice identified are 4-amino-acetophenone, N,N-dimethyl-2-chloroacetoacetamide, 3-beta-acetoxy-5-etienic acid, 6,10-dimethylundeca-5,9,-diene2-one, Phenyl-3-buten and 4-Phenyl butanone. There were significant differences in the microbial counts of the treated and untreated samples. Treated samples experienced little or no growth over the period of storage. The results validate previous reports that A. danielli extract has great preservative potentials. There were significant differences (P < 0.5) for colour and overall acceptability of the samples. When A. danielli extract was added to soymilk-based fruit juice, the microbiological and sensory qualities were found to be better preserved than that of the untreated samples.

A. danielli Soymilk-Based Juice Microbial load Shelf Life

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[1]  Adegoke, G.O (2000): Understanding Food Microbiology. First Edition, Shalom Prints, Ibadan. Pg. 49-65.
[2]  Adegoke, G.O.; Gbadamosi, R; Evwoerhuhoma, F; Uzo-Peters, P.I; Falade, K.O, Itiola, 0: Moody, 0 and Skura, B. (2002): protection of Maize (Zeamays) and Soyabean (Glycinemax) using Aframomum danielli. European Food Research and Technol. 214, 408-411.
[3]  Adegoke, G.O, Iwahashi, H. Komatsu, Y. (1997): Inhibition of Saccharomyces cerevisae by combination of hydrostatic pressure and monoterpenes. J. Food Science 67: 404-405.
[4]  Adegoke, G.O; Gopalakrishna, A.G. (1998): Extraction and identification of antiodixants from the spice Aframomum danielli. J. Am. Oil Chern. Soc. 75: 1047-1052.
[5]  Adefegha SA, Oboh G (2011a). Enhancement of Total Phenolics and Antioxidant Properties of Some Tropical Green Leafy Vegetables by Steam Cooking. J. Food Process Preserv. 35 (5): 615-622.
[6]  Adegoke, G.O. Skura, B.J. (1994); Nutritional, Profile and Antimicrobial Spectrum of the spice Aframomum danielli, plant food Human Nut. 45: 175-182.
[7]  Al-Jedah and J.H. and Robinson RK (2002). Nutritional Value and Microbiological Safety of Fresh Juices sold through Retail Outlets in Qatar. Pakistan Journal of Nutritional 1: 79-81.
[8]  Allende A. Tomas-Barberan FA, Gil MI. Minimal processing for healthy traditional foods. Trends in Food Science and Technology. 2006; 17: 513-519.
[9]  AOAC (2005): Official Methods of Analysis of Association of Official Analytical Chemist International, Washington (D.C.).
[10]  Ashaye A.O., Taiwo OO and Adegoke GO (2007). Effect of Local Preservative (Aframonium danieli) on the Chemical and Sensory Properties of Stored “warankassi” African Journal of Agricultural Research 1: 010-016.
[11]  Aworh, O.C. and Olorunda, A.O. (1988). Packaging and Storing Technology of fresh fruits and vegetables with special references to tropical conditions. Proc. National Workshop on Improved Packaging and Storage system for fruits and vegetables in Nigeria. Pg. 75-91.
[12]  Battcock M, Axain-Ali S, Axtell B and Fellow PI (1998). Successful Approaches to Training in Food Processing. IT Publications, pg B6.
[13]  Ceylan E, Fung DYC Sabah J.R. (2004). Antimicrobial Activity and Synergistic Effect of Cinammon with Sodium Benzoate or Potassium Sorbate in Controlling Eschenchia coli 0157: H7 in Apple Juice. Journal of Food Science 69: 102-105.
[14]  Chang SS, Ostic-Matijasevic BO, Hsieh QA and Huang CL (1977). Natural antioxidant from rosemary and sage. J. Food Sci., 42: 1102-1106.
[15]  Clade MI (1997). Food Nutrition and the Prevention of Cancer; a global perspective, American Institute for Cancer Research/World Cancer Research Fund; 15: 523-6.
[16]  Corbo MR, Bevilacqua A. Campaniello D, D’Amato D, Speranza B. Sinigaglia M. Prolonging microbial shelf life of foods through the use of natural compounds and non-thermal approaches- a review. International Journal of Food Science and Technology, 2009; 44: 223-241.
[17]  Crook L.R. and Boylston T.D. (2004). Flavour Characteristics of Irradiated Apple Cider during Storage: Effect of Packaging Materials and Sorbate Addition. Journal of Food Science 69: 557-562.
[18]  Delgado AE, and Sun DW (2000). Heat and mass transfer for predicting freezing processes, a review. Journal of Food Engineering, 47: 157-174.
[19]  Elez P, Soliva RC, Martins O. 2006. Comparative study on shelf life of orange juice processed by high intensity pulsed electric fields or heat treatment. Euro Food Res Technol 222: 321-9.
[20]  Fasoyiro, S.B. Adegoke, G.O., Ashaye, O.A. Obatolu, V.A. and Owolade, O.F. 2001. Antimicrobial Characteristics of Aframomum danielli spice on Escherichia coli and Staphylococcus aureus. Moor Journal of Agricultural Research. 2: 159-161.
[21]  Ghenghesh, K.S. Khalifa B., El-Ammin, W. Saleha, E. and Zalmum, A. 2005. Microbiological quality of fruit juices sold in Tripoli-Libya. Food control. 15: 855-858.
[22]  Hussain I., A, Shakir I, A (2008): Combine Effect of Potassium Sorbate and Soduim Benzoate on Individual and Blended Juices of Apricot and Apple Juices grown in Azad Jammu and Kashmir. Pakistan Journal of Nutrition 7: 181-185.
[23]  Ito N, Fukushima S, Hasegawa A, Shibata M and Ogiso T (1985). Carcinigenicity of butylated hydroxylanisole in F344 rats. J. Natl. Cancer Inter., 70: 343-347.
[24]  Jia M., Zhang QH, Min DB. 1999, Pulsed Electric Field Processing Effects on Flavour Compounds and Micro-organism of Orange Juice. Food Chem 65: 445-51. Netherlands.
[25]  Lavinas FC, Miguel MA, Lopes ML, Valente-Mesquita VL (2008). Effect of high hydrostatic pressure on cashew apple (Anacardium occidentale L.) juice preservation. J. Food Sci. 73: 273-277.
[26]  Levi, A; Gagel, S. and Juven, B. (1983): Intermediate moisture tropical fruit products for developing countries. 1. Technological data on papaya. J. Food Tech. 18 (6): 667-685.
[27]  Lu M, Yuan B, Zeng M, Chen (2011). Antioxidant Capacity and Major Phenolic Compounds of Spices Commonly Consumed in China. Food Res. Int. 44: 530-536.
[28]  Luedtke AN and Powell DA (2002). A Review of North American Apple Cider Association in Eschenchia coli 0157: H7 outbreaks. Dairy Food Environment Sanitation 22: 590-598.
[29]  Martin AD and Gilbert D (1968). Enzyme changes accompanying liver enlargement in rats treated with 3-tert-butyl-4-hydroxyanisole. Biochem J. 10: 22-27.
[30]  Miliauskas G, Venskutonic P, and van Beek TA (2004). Screening of radical scavenging activity of some medicinal and aromatic plants extracts. Food Chem., 82: 231-237.
[31]  Miliauskas G, Venskutonis PR, and van Beek TA (2004). Screening of radical scavenging activity of some medicinal plant and aromatic plants extracts. Food Chem., 85: 231-237.
[32]  Min S, Jin T, Zhang QH. 2003b. Commercial scale pulsed electric field processing of tomato juice. J Agric Food Chem 51: 3338-44.
[33]  Min S, Jin ZT, Min SK, Yeom H, Zhang QH. 2003a. Commercial-scale pulsed electric field processing of orange juice. J Food Sci 68 (4): 1265-71.
[34]  Oboh G, Rocha JBT (2007). Antioxidant in Foods: A New Challenge for Food Processors. Leading Edge Antioxidants Research, Nova Science Publishers Inc. New York US, 35-64.
[35]  Odukoya AO, Ilori OO, Sofidiya MO, Aniunoh OA, Lawal BM, Tade IO (2005). Antioxidant Activity of Nigerian Dietary Spices. Electr. J. Environ. Agric Food Chem. 4 (6): 1086-1093.
[36]  Ogunwolu, S.O. and Adio, S.O. 2003. Preservative effect of A. danielli on the storage stability of cashew juice. Proceeding of the 27th annual conference of Nigerian Institute of Food Science and Technology (NIFST). 13-17th Oct. held in Kano, Nigeria. Uzochukwu, E. Ed.
[37]  Olivas GI, Barbosa-Canovas GV. Edible coatings for fresh-cut fruits. Critical Reviews in Food Science and Nutrition. 2005;45: 657 – 663.
[38]  Omemu AM, Edema M.O., Atayese A.O. and Obadina A.O. (2006). A Survey of the Microflora of Roselle (Hibiscus sabdariffa) and the resulting “Zobo” Juice. African Journal of Biotechnology. 3: 254-259.
[39]  Osawa T, Hirotak K, Hahiwara H and Shibamoto T (1992). A novel antioxidant isolated from young green barley leaves. J. Agric. Food Chem., 1135-1138.
[40]  Rivas A, Rodrigo D, Martinez A, Barbosa-Canovas GV, Rodrigo M. 2006. Effect of PEF and heat pasteurization on the physico-chemical characteristics of blended orange and carrot juice. Lebensm Wiss Technol 39: 1163-70.
[41]  Shan B, Cai YZ, Sun M and Corke H (2005). Antioxidant Capacity of 26 Spice Extracts and Characterization of their Phenolic Constituents. J. Agric. Food Chem. 53 (20): 7749-7759.
[42]  Steinmetz KA, Potter ID (1996). Vegetables, fruits, and cancer prevention: a review. J AM Diet Assoc. 96: 1027-39.
[43]  Stone H, Sidel JL (1992). Sensory evaluation practices. 2nd ed. San Diego: Elsevier, pp. 336.
[44]  Torres JA, Velazquez G (2005). Commercial opportunities and research challenges in the high pressure processing of foods. Journal of food Enhineering, 67, pp. 95-112.
[45]  Viuda-Martos M, Ruiz-Navajas Y, Fernandez-Lopez J, Perez-Alvarez JA (2011). Spices as Functional Foods. Crit. Rev. Food Science Food Saf. 51: 13-28.
[46]  Weng XC and Gordon MH (1992). Antioxidant activity of quinines extracted from tanshen (Salvia mitorrhiza Bunge). J. Agric. Food Chem., 40: 1331-1336.
[47]  Wong, P. Yusof S. Ghazali, H.M. and Cheman. Y.B. 2003.Optimization of hot water extraction of roselle juice using response surface methodology: a comparative study with other extraction methods. Journal of Science of Food and Agriculture 83: 1273-1278.
[48]  Wojdylo A, Oszmianski J, Czemerys R (2007). Antioxidant activity and Phenolics Compounds in 32 Selected Herbs. Food Chem. 105 (3): 940-949.
[49]  Yeom HW, Streaker CB, Zhang QH, Min DB. 2000. Effects of pulsed electric fields on the quality of orange juice and comparism with heat pasteurization. J Agric Food Chem 48: 4597-605.
[50]  Zaika, L.L. 1988. Spices and Herbs: their antimicrobial activity and its determination. Journal of Food Safety 9: 97-117.