An Undergraduate Experiment Using Cyclodextrin – Assisted Sensitive Fluorescence Detection and Quantitation of Dapsone Drug in Wastewater Samples

Mohammed A. Meetani^1, , Ahmad Alhalabi², Mohammed K. Al-tabaji¹, Noor I Albadawi¹, Nada Elmari¹, Soleiman Hisaindee¹, Abdullah Al-Hemyari¹ and Munjed Maraqa²

¹Chemistry Department, College of Science, United Arab Emirates University, P.O. Box 15551 Al-Ain, UAE

²Civil and Environmental Engineering Department, United Arab Emirates University, P.O. Box 15551 Al-Ain, UAE

Cite this paper:
Mohammed A. Meetani, Ahmad Alhalabi, Mohammed K. Al-tabaji, Noor I Albadawi, Nada Elmari, Soleiman Hisaindee, Abdullah Al-Hemyari and Munjed Maraqa. An Undergraduate Experiment Using Cyclodextrin – Assisted Sensitive Fluorescence Detection and Quantitation of Dapsone Drug in Wastewater Samples. World Journal of Chemical Education. 2019; 7(4):242-247. doi: 10.12691/wjce-7-4-2

Abstract

The guest-host interaction between Dapsone drug and β-cyclodextrin (β-CD) was investigated using fluorescence spectroscopy, ¹H-NMR, and liquid chromatography with fluorescence detection. The optimized conditions for the interaction were investigated by spectrofluorometry and were found to be at 0.2 mg/mL (0.176 mM) of β-CD and pH 8.8. For these conditions, very low concentration of Dapsone drug of 2.4 ng/mL (12.97 nM) can be detected. The standard addition method was utilized to detect Dapsone in influent and effluent wastewater samples in the sub parts per billion concentration range by HPLC-FLD using β-CD as an additive in the mobile phase.

Keywords:
dapsone β-cyclodextrin fluorescence guest-host interaction wastewater

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