Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: https://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
Open Access
Journal Browser
Go
Journal of Food and Nutrition Research. 2017, 5(11), 809-816
DOI: 10.12691/jfnr-5-11-3
Open AccessArticle

Effects of Ultrasound Pretreatment and Ageing Processing on Quality and Tenderness of Pork Loin

C.K. Yeung1 and S.C. Huang1,

1Food Industry Research and Development Institute, Hsinchu, Taiwan, ROC

Pub. Date: October 28, 2017

Cite this paper:
C.K. Yeung and S.C. Huang. Effects of Ultrasound Pretreatment and Ageing Processing on Quality and Tenderness of Pork Loin. Journal of Food and Nutrition Research. 2017; 5(11):809-816. doi: 10.12691/jfnr-5-11-3

Abstract

Tenderness is a primary indicator of meat quality, which directly influences consumer acceptance of the meat. Ultrasound can effectively increase the tenderness of red meat, such as pork and beef, by breaking up muscle fibers and connective tissues through mechanical force, thus making it a basis for physical tenderization of meat to improve meat quality. In this study, ultrasound (2200 W, 15 kHz) was applied in the processing of pork loin to explore the influences of varying treatment durations (0-6 minutes) and posttreatment aging durations (0-2 days) on the tenderness, texture profile, physical and chemical qualities (myofibrillar fragmentation index, or MFI, and rate of marinade absorption), and sensory evaluation (9-point hedonic scale) of meat. The results indicated that ultrasonic treatment (2200 W) for 6 minutes can effectively tenderize the meat by increasing the MFI to 15.1%, lowering the hardness in texture profile analysis (TPA) to 87.6%, and reducing shear force to 87.9%. Moreover, if pork loin is allowed to age for 48 hours following 6 minutes of ultrasonic treatment, shear force can be further reduced to 72.3%. The sensory evaluation scores for texture, tenderness, and overall acceptance of pork loin increased with the elongated ultrasonic treatment.

Keywords:
meat product physical tenderization texture shear force cooking loss sensory

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  Jane S.T., 2015 Annual report of food industry, Industry and Technology Intelligence Services, Taiwan, 2015.
 
[2]  Miller, M.F., Carr, M.F., Ramsey, C.B., Crockett, K.L. and Hoover, L.C. “Consumer thresholds for establishing the value of beef tenderness,” Journal of Animal Science, 79.3062-3068. 2001.
 
[3]  Istrati, D., “The influence of enzymatic tenderi- zation with papain on functionalproperties of adult beef,” Journal of Agroalimentary Processes and Technologies, 14. 140-146. 2008.
 
[4]  Rubio-Celorio, M., Fulladosa, E., Garcia-Gil, N. and Bertram, H.C., “Multiple spectroscopic approach to elucidate water distribution and water-protein interactions in dry-cured ham after high pressure processing,” Journal of Food Engineering, 169. 291-297. 2016.
 
[5]  Goll, D.E., Boehm, M.L., Geesink Geert, H. and Thompson, V.F., “What causes postmortem tenderization,” American Meat Science Association, 50.60-67. 1997.
 
[6]  Lawrie, R. and Ledward, D., Lawrie's meat science (7th ed.), Cambridge: Woodhead Publishing Limited, England, 2006.
 
[7]  Meek, K.I., Calus J.R., Duncan, S.E., Marriott, N.G., Solomon, M.B., Kathman, S.J. and Marini, M.E, “Quality and Sensory Characteristics of Selected Post-Rigor, Early Deboned Broiler Breast Meat Tenderized Using Hydrodynamic Shock Waves,” Poultry Science, 79.126-136. 2000.
 
[8]  Barekat, S. and Soltanizadeh, N., “Improvement of meat tenderness by simultaneous application of high-intensity ultrasonic radiation and papain treatment,” Innovative Food Science and Emerging, 39. 223-229. 2017.
 
[9]  Nollet, L. and Toldra, F., Advanced technologies for meat processing, CRC Press, Taylor & Francis, USA, 353-358. 2006.
 
[10]  Siró I., Vén, C., Balla, C., Jónás, G., Zeke, I. and Friedrich, L., “Application of an ultrasonic assisted curing technique for improving the diffusion of sodium chloride in porcine meat,” Journal of Food Engineering, 91. 353-362. 2009.
 
[11]  Wang, Z.Y., Xu, W.W., Kang, N.Q., Shen, W. and Zhang, D.Q., “Microstructural, protein denaturation and water holding properties of lamb under pulse vacuum brining,” Meat Science, 113. 132-138. 2016.
 
[12]  Gou, P., Comaposada, J. and Arnau. J., “NaCl content and temperature effects on moisture diffusivity in the gluteus medius muscle of pork ham,” Meat Science, 63. 29-34. 2003.
 
[13]  Graiver, N., Pinotti, A., Califano, A. and Zaritzky, N., “Diffusion of sodium chloride in pork tissue,” Journal of Food Engineering, 77(4). 910-918. 2006.
 
[14]  Cárcel, J.A., Benedito, J., Bon, J. and Mulet, A., “High intensity ultrasound effects on meat brining,” Meat Science, 76(4). 611-619. 2007.
 
[15]  Got, F., Culioli, J., Berge, P., Vignon, X., Astruc, T., Quideau, J. and Lethiecq, M., “Effects of high-intensity high-frequency ultrasound on ageing rate, ultrastructure and some physico-chemical properties of beef,” Meat Science, 51(1). 35-42. 1999.
 
[16]  Marcos, B. and Mullen, A.M., “High pressure induced changes in beef muscle proteome: correlation with quality parameters,” Meat Science, 97. 11-20. 2014.
 
[17]  Jayasooriya, S.D., Torley, P.J., D’Arcy, B.R. and Bhandari, B.R., “Effect of high power ultrasound and ageing on the physical properties of bovine Semitendinous and Longissimus muscles,” Meat Science, 75(4). 628-639. 2007.
 
[18]  Kang, D.C., Gao, X.Q., Ge, Q.F., Zhou, G.H. and Zhang, W.G., “Effects of ultrasound on the beef structure and water distribution during curing through protein degradation and modification,” Ultrasonics Sonochemistry, 38.317-325. 2017.
 
[19]  Xiong, G., Zhang, L., Zhang, W. and Wu, J., “Influence of ultrasound and proteolytic enzyme inhibitors on muscle degradation, tenderness, and cooking loss of hens during aging,” Czech Journal of Food Sciences, 30(3).195-205. 2012.
 
[20]  Ashokkumar, M., “The characterization of acoustic cavitation bubbles - an overview,” Ultrasonics Sonochemistry, 18. 864-872. 2011.
 
[21]  Chandrapala, J., Oliver, C., Kentish, S. and Ashokkumar, M., “Ultrasonics in food processing,” Ultrasonics Sonochemistry, 19. 975-983. 2012.
 
[22]  Fan, D., Huang, L., Li, B., Huang, J., Zhao, J., Yan, B., Zhou, W., Zhang, W. and Zhang, H., “Acoustic intensity in ultrasound field and ultrasound-assisted gelling of surimi,” Food Science and Technology, 75. 497-504. 2017.
 
[23]  Kang D.C., Wang, A.R., Zhou, G.H., Zhang, W.G., Xu, S.M. and Guo, G.P., “Power ultrasonic on mass transport of beef: effects of ultrasound intensity and NaCl concentration,” Innovative Food Science and Emerging Technologies, 35. 36-44. 2016.
 
[24]  Awad, T., Moharram, H., Shaltout, O., Asker, D. and Youssef, M., “Applications of ultrasound in analysis, processing and quality control of food: A review,” Food Research International, 48(2). 410- 427. 2012.
 
[25]  Kolsky, H., Stress Waves in Solids, Dover Publishing, New York, 1980.
 
[26]  Turantaş, F., Kılıç, G.B. and Kılıç. B., “Ultrasound in the meat industry: general applications and decontamination efficiency,” International journal of food microbiology, 198. 59-69. 2015.
 
[27]  McDonnell, C.K., Allen, P., Morin, C. and Lyng, J.G., “The effect of ultrasonic salting on protein and water-protein interactions in meat,” Food Chemistry, 147. 245-251. 2014.
 
[28]  Raloff J., Ka-Boom! A shockingly unconventional meat tenderizer, Science News, 23: 366-369. 1998.
 
[29]  Olson, D.G., Parish, J.F.C. and Stromer. M.H., “Myofibril fragmentation and shear resistance of three bovine muscles during postmortem storage,” Journal of Food Science, 41. 1036-1041. 1976.
 
[30]  Hopkins, D., Littlefield, L.P.J. and Thompson, J.M., “A research note on factors affecting the determination of myofibrillar fragmentation,” Meat Science, 56. 19-22. 2000.
 
[31]  Gornall, A.G., Bardawill, C.J. and David, M.M., “Deterimination of serum proteins by means of the biuret reaction,” Journal of Biological Chemistry, 177. 751-766. 1949.
 
[32]  Huang, S.C., Shiau, C.Y., Liu, T.E., Chu C.L. and Hwang. D.F., “Effects of rice bran on sensory and physico-chemical properties of emulsified pork meatballs,” Meat Science, 70. 613-619. 2005.
 
[33]  Chen, L., Zhou, G.H. and Zhang, W.G., “Effects of high oxygen packaging on tenderness and water holding capacity of pork through protein oxidation,” Food and Bioprocess Technology, 8. 2287-2297. 2015.
 
[34]  Meilgaard, M., Civille G.V. and Carr, B.T., Sensory evaluation techniques, 2nd Ed, CRC Press, USA, 1991.
 
[35]  Dolatowski, Z.J., “Ultraschall. 2. Einfluss von ultraschall auf die Mikrostruktur von nuskelgewebe bei der poekehuig. Die Fleischwirtschaft, 68.1301- 1303. 1988.
 
[36]  Koohmaraie, M., Seideman, S.C., Schollmeyer, J.E., Dutson, T.R. and Grouse, J.D., “Effect of post-mortem storage on Ca++ dependent proteases, their inhibitor and myofibril fragmentation,” Meat Science, 19(3). 187-196. 1987.
 
[37]  Olson, D.G., Parrish F. C. Jr., Dayton, W.R. and Goll, D.E., “Effect of postmortem storage and calcium activated factor on the myofibrillar proteins of bovine skeletal muscle,” Journal of Food Science, 42. 117-124. 1977.
 
[38]  Zuckerman, H. and Solomon, M.B., “Ultrastructure changes in bovine longissimus muscle caused by the Hydrodyne process,” Journal of Muscle Foods, 9. 419-426. 1998.
 
[39]  Huff-Lonergan, E. and Lonergan, S.M., “Mechanisms of water-holding capacity of meat: the role of postmortem biochemical and structural changes,” Meat Science, 71. 194-204. 2005.
 
[40]  Graiver, N., Pinotti, A., Califano, A. and Zaritzky, N., “Mathematical modeling of the uptake of curing salts in pork meat,” Journal of Food Engineering, 95(4). 533-540. 2009.
 
[41]  Vestergaard, C., Andersen, B.L. and Adler-Nissen, J., “Sodium diffusion in cured pork determined by 22Na radiology,” Meat Science, 76(2). 258-265. 2007.
 
[42]  Vestergaard, C., Erbou, S.G., Thauland, T., Adler-Nissen, J. and Berg, P., “Salt distribution in dry-cured ham measured by computed tomography and image analysis,” Meat Science, 69(1). 9-15. 2005.
 
[43]  Wang, D., Tang, J. and Correia, L.R., “Salt diffusivities and salt diffusion in farmed Atlantic salmon muscle as influenced by rigor mortis,” Journal of Food Engineering, 43(2). 115-123. 2000.
 
[44]  Ozuna, C., Puig, A., Garcia-Perez, J.V., Mulet, A. and Cárcel, J.A., “Influence of high intensity ultrasound application on mass transport, microstructure and textural properties of pork meat (Longissimus dorsi) brined at different NaCl concentrations,” Journal of Food Engineering, 119(1). 84-93. 2013.
 
[45]  Kuijpers, M.W.A., Kemmere, M.F. and Keurentjes, J.T.F., “Calorimetric study of the energy efficiency for ultrasound-induced radical formation,” Ultrasonics Sonochemistry, 40. 675-678. 2002.
 
[46]  Saleem R. and Ahmad, R., “Effect of ultrasonication on secondary structure and heat induced gelation of chicken myofibrils,” Journal of Food Science and Technology, 53. 3340-3348. 2016.
 
[47]  Aaslyng, M.D., Bejerholm, C., Ertbjerg, P., Bertram, H.C. and Andersen, H.J., “Cooking loss and juiciness of pork in relation to raw meat quality and cooking procedure,” Food Quality and Preference, 14. 277-288. 2003
 
[48]  Pohlman, F.W., Dikeman, M.E. and Kropf, D.H., “Effects of high intensity ultrasound treatment, storage time and cooking method on shear, sensory, instrumental color and cooking properties of packaged and unpackaged beef pectoral & muscle,” Meat Science, 45(3). 89-100. 1997.
 
[49]  Smith, N.B., Cannon, J.E., Novakofski, J.E., McKeith, F.K. and O’Brien, W.D. Jr., Tenderization of semitendinosus muscle using high intensity ultrasound, in: Ultrasonics Symposium, Lake Buena Vista, USA, 1371-1373, 1991.
 
[50]  Dickens J.A., Lyon C.E. and Wilson, R.L., “Effect of Ultrasonic Radiation on Some Physical Characteristics of Broiler Breast Muscle and Cooked Meat,” Poultry Science, 70(2). 389-396. 1991.
 
[51]  Claus, J.R., Schilling, J.K., Marriott, N.G. and Duncan, S.E., Solomon, M.B. and Wang H., “Hydrodynamic shockwave tenderization effects using a cylinder processor on early deboned broiler breasts,” Meat Science. 58. 287-292. 2001.
 
[52]  Lyng, J.G., Allen, P. and McKenna, B.M., “The influence of high intensity ultrasound baths on aspects of beef tenderness,” Journal of Muscle Foods, 8. 237-249. 1997.
 
[53]  Koohmaraie, M. and Geesink, G.H., “Contribution of postmortem muscle biochemistry to the delivery of consistent meat quality with particular focus on the calpain system,” Meat Science, 74. 34-43. 2006.
 
[54]  Moeller, P.W., Fields, P.A., Dutson, T.R., Landmann, W.A. and Carpenter, Z.L., “Effect of high temperature conditioning on sub-cellular distribution and levels of lysosomal enzymes,” Journal of Food Science, 41. 216-217. 1976.
 
[55]  McDonnell, C.K., Lyng, J.G. and Allen, P., “The use of power ultrasound for accelerating the curing of pork,” Meat Science, 98(2). 142-149. 2014.
 
[56]  Ojha, K.S., Keenan, D.F., Bright, A., Kerry, J.P. and Tiwari, B.K., “Ultrasound assisted diffusion of sodium salt replacer and effect on physicochemical properties of pork meat,” International Journal of Food Science and Technology, 51(1).37-45. 2016.