Novel Hydrolyzed Porcine and Fish Collagen Beverage Improved Collagen, Elasticity, Moisture, Spots: A Randomized, Double-blind, Placebo-controlled Trial

Yung-Kai Lin^{1, 2}, Chia-Hua Liang³, Yung-Hsiang Lin⁴, Shu-Ting Chan⁴ and Chi-Fu Chiang^4,

¹Institute of Food Safety and Risk Management, National Taiwan Ocean University, Keelung, Taiwan

²Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan;Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan

³Department of Cosmetic Science and Institute of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan, Taiwan

⁴Research & Design Center, TCI CO., Ltd., Taipei, Taiwan

Cite this paper:
Yung-Kai Lin, Chia-Hua Liang, Yung-Hsiang Lin, Shu-Ting Chan and Chi-Fu Chiang. Novel Hydrolyzed Porcine and Fish Collagen Beverage Improved Collagen, Elasticity, Moisture, Spots: A Randomized, Double-blind, Placebo-controlled Trial. Journal of Food and Nutrition Research. 2022; 10(4):305-312. doi: 10.12691/jfnr-10-4-7

Abstract

Background: As the population ages, oral hydrolyzed collagen supplements are gaining traction. However, few clinical studies on the skin health of hydrolyzed porcine collagen combined with fish collagen. Objective: This study investigates whether TripleUp collagen beverage has skincare and anti-inflammatory effects. Methods: Using collagen beverage to treat epidermal keratinocytes and examine ROS, HA, inflammatory factor, and moisturizing-related gene expressions (Tgm1, Krt14, FLG-F, GBA, HAS3). Fifty subjects were recruited into a placebo and collagen beverage group for four weeks and then examined skin condition at 0, 2, 4 weeks. Results: TripleUp collagen beverage decreased IL-8 and ROS expression and increased moisturizing-related gene expressions. In addition, collagen beverages increase skin collagen, elasticity, moisture, and decreased spots. Conclusions: Thus, TripleUp collagen beverage included hydrolyzed porcine, and fish collagen and vitamin C can improve skin condition.

Keywords:
collagen fish skin porcine vitamin C

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]	Zhang S, Duan E. Fighting against Skin Aging: The Way from Bench to Bedside. Cell Transplant. 2018; 27: 729-38.
[2]	D'Orazio J, Jarrett S, Amaro-Ortiz A, Scott T. UV radiation and the skin. Int J Mol Sci. 2013; 14: 12222-48.
[3]	Pillai S, Oresajo C, Hayward J. Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation - a review. Int J Cosmet Sci. 2005; 27: 17-34.
[4]	Papakonstantinou E, Roth M, Karakiulakis G. Hyaluronic acid: A key molecule in skin aging. Dermatoendocrinol. 2012; 4: 253-8.
[5]	Bolke L, Schlippe G, Gross J, Voss W. A Collagen Supplement Improves Skin Hydration, Elasticity, Roughness, and Density: Results of a Randomized, Placebo-Controlled, Blind Study. Nutrients. 2019; 11.
[6]	Shoulders MD, Raines RT. Collagen structure and stability. Annu Rev Biochem. 2009; 78: 929-58.
[7]	Leon-Lopez A, Morales-Penaloza A, Martinez-Juarez VM, Vargas-Torres A, Zeugolis DI, Aguirre-Alvarez G. Hydrolyzed Collagen-Sources and Applications. Molecules. 2019; 24.
[8]	Evans M, Lewis ED, Zakaria N, Pelipyagina T, Guthrie N. A randomized, triple-blind, placebo-controlled, parallel study to evaluate the efficacy of freshwater marine collagen on skin wrinkles and elasticity. 2021; 20: 825-34.
[9]	Lupu MA, Gradisteanu Pircalabioru G, Chifiriuc MC, Albulescu R, Tanase C. Beneficial effects of food supplements based on hydrolyzed collagen for skincare (Review). Exp Ther Med. 2020; 20: 12-7.
[10]	Proksch E, Segger D, Degwert J, Schunck M, Zague V, Oesser S. Oral supplementation of specific collagen peptides has beneficial effects on human skin physiology: a double-blind, placebo-controlled study. Skin Pharmacol Physiol. 2014; 27: 47-55.
[11]	Kawashima A, Sekizawa A, Koide K, Hasegawa J, Satoh K, Arakaki T, et al. Vitamin C Induces Oxidative Stress and Paradoxically Stimulates the Apoptotic Gene Expression in Extravillous Trophoblasts Derived From First-Trimester Tissue. Reprod Sci. 2015; 22: 783-90.
[12]	Pullar JM, Carr AC, Vissers MCM. The Roles of Vitamin C in Skin Health. Nutrients. 2017; 9.
[13]	Ricard-Blum S. The collagen family. Cold Spring Harb Perspect Biol. 2011; 3: a004978.
[14]	Chattopadhyay S, Raines RT. Review collagen-based biomaterials for wound healing. Biopolymers. 2014; 101: 821-33.
[15]	Zhu L, Xie Y, Wen B, Ye M, Liu Y, Imam KMSU, et al. Porcine bone collagen peptides promote osteoblast proliferation and differentiation by activating the PI3K/Akt signaling pathway. Journal of Functional Foods. 2020; 64: 103697.
[16]	Nurilmala M, Hizbullah HH, Karina E, Kusumaningtyas E, Ochiai Y. Characterization and Antioxidant Activity of Collagen, Gelatin, and the Derived Peptides from Yellowfin Tuna (Thunnus albacares) Skin. Mar Drugs. 2020; 18.
[17]	Venkatesan J, Anil S, Kim SK, Shim MS. Marine Fish Proteins and Peptides for Cosmeceuticals: A Review. Mar Drugs. 2017; 15.
[18]	Mary L, Smith S. Collagen type II, alpha 1 protein: A bioactive component of shark cartilage. International immunopharmacology. 2012; 15.
[19]	Dupont E, Savard PE, Jourdain C, Juneau C, Thibodeau A, Ross N, et al. Antiangiogenic properties of a novel shark cartilage extract: potential role in the treatment of psoriasis. J Cutan Med Surg. 1998; 2: 146-52.
[20]	Lin P, Hua N, Hsu YC, Kan KW, Chen JH, Lin YH, et al. Oral Collagen Drink for Antiaging: Antioxidation, Facilitation of the Increase of Collagen Synthesis, and Improvement of Protein Folding and DNA Repair in Human Skin Fibroblasts. Oxid Med Cell Longev. 2020; 2020: 8031795.
[21]	Padayatty SJ, Levine M. Vitamin C: the known and the unknown and Goldilocks. Oral Dis. 2016; 22: 463-93.
[22]	Ellul MS, Rahmat A, Fatimah I, Khaza'ai H, Abed Y. Effect of vitamin C on inflammation and metabolic markers in hypertensive and/or diabetic obese adults: a randomized controlled trial. Drug Des Devel Ther. 2015; 9: 3405-12.
[23]	Rodriguez Cruz SI, Phillips MA, Kultz D, Rice RH. Tgm1-like transglutaminases in tilapia (Oreochromis mossambicus). PLoS One. 2017; 12: e0177016.
[24]	Sumer C, Boz Er AB, Dincer T. Keratin 14 is a novel interaction partner of keratinocyte differentiation regulator: receptor-interacting protein kinase 4. Turk J Biol. 2019; 43: 225-34.
[25]	McAleer MA, Jakasa I, Raj N, O'Donnell CPF, Lane ME, Rawlings AV, et al. Early-life regional and temporal variation in filaggrin-derived natural moisturizing factor, filaggrin-processing enzyme activity, corneocyte phenotypes and plasmin activity: implications for atopic dermatitis. Br J Dermatol. 2018; 179: 431-41.
[26]	Boer DEC, van Sweden J, Al-Khakany H, Melnik E, van Dijk R, Abdallah S, et al. Skin of atopic dermatitis patients shows disturbed β-glucocerebrosidase and acid sphingomyelinase activity that relates to changes in stratum corneum lipid composition. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 2020; 1865: 158673.
[27]	Kuo YZ, Fang WY, Huang CC, Tsai ST, Wang YC, Yang CL, et al. Hyaluronan synthase 3 mediated oncogenic action through forming inter-regulation loop with tumor necrosis factor-alpha in oral cancer. Oncotarget. 2017; 8: 15563-83.
[28]	Kang MC, Yumnam S, Kim SY. Oral Intake of Collagen Peptide Attenuates Ultraviolet B Irradiation-Induced Skin Dehydration In Vivo by Regulating Hyaluronic Acid Synthesis. Int J Mol Sci. 2018; 19.
[29]	Miyanaga M, Uchiyama T, Motoyama A, Ochiai N, Ueda O, Ogo M. Oral Supplementation of Collagen Peptides Improves Skin Hydration by Increasing the Natural Moisturizing Factor Content in the Stratum Corneum: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial. Skin Pharmacol Physiol. 2021; 34: 115-27.
[30]	Osawa Y, Mizushige T, Jinno S, Sugihara F, Inoue N, Tanaka H, et al. Absorption and metabolism of orally administered collagen hydrolysates evaluated by the vascularly perfused rat intestine and liver in situ. Biomed Res. 2018; 39: 1-11.
[31]	Choi FD, Sung CT, Juhasz ML, Mesinkovsk NA. Oral Collagen Supplementation: A Systematic Review of Dermatological Applications. J Drugs Dermatol. 2019; 18: 9-16.
[32]	Inoue N, Sugihara F, Wang X. Ingestion of bioactive collagen hydrolysates enhance facial skin moisture and elasticity and reduce facial aging signs in a randomized, double-blind placebo-controlled clinical study. J Sci Food Agric. 2016; 96: 4077-81.