Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: https://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
Open Access
Journal Browser
Go
Journal of Food and Nutrition Research. 2018, 6(6), 406-413
DOI: 10.12691/jfnr-6-6-9
Open AccessArticle

Microbiological and Physicochemical Quality of Honeys from South and South-West of Niger

Laredj Hocine1, 2, , Berakaa Tefiel2, Ali Moustapha2, Amine Allaoui2 and Kheïra Benamara2

1Laboratory of Agro-Biotechnology and Nutrition in Semi-Arid Areas

2Faculty of Science of Nature and Life, University Ibn Khaldoun Tiaret, Algeria

Pub. Date: July 11, 2018

Cite this paper:
Laredj Hocine, Berakaa Tefiel, Ali Moustapha, Amine Allaoui and Kheïra Benamara. Microbiological and Physicochemical Quality of Honeys from South and South-West of Niger. Journal of Food and Nutrition Research. 2018; 6(6):406-413. doi: 10.12691/jfnr-6-6-9

Abstract

With all the complexity of the biological composition of honey, our work focused on the microbiological (bacteriological and mycological) and physicochemical characterization of Nigerian honeys. The results showed a satisfactory bacteriological composition of honey samples in accordance with the standards, high mold and a very high yeast load making these honeys of mediocre quality and unfit for human consumption. Isolated yeast species: Schizosaccharomyces plombe, Zygosaccharomyces rouxii, Kluyveromyces thermotolerans, Saccharomyces cerevisiae and the isolated molds are: Aspergillus (Asp) fumigatus, Asp. candidus, Asp. niger, Asp. versicolor Asp. nidulans and Mucor. The study revealed that all honeys had pH, water content, electrical conductivity, ash content, free acidity, respectively, ranging within 3.8-4.95, 47.2-20 %, 0.484-0.669 mS/cm, 0.143-0.262 g/100g, 41.7-83.05 meq/kg. The quality of Nigerian honeys can be improved by applying Good Agricultural Practices (GAP) and Good Manufacturing Practices (GMP).

Keywords:
quality microbiological physicochemical honey Niger

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  Bogdanov, S., “The honey book”, Bee Product Science, 2011.
 
[2]  Jean-Prost, P., Le Conte, Y, “Apiculture, connaitre l’abeille, Conduire le rucher,” 7ème éd Lavoisier, France, 2005, 698.
 
[3]  Snowdon, J.A, “The microbiology of honey - meeting your buyers,” specifications (Why they do what they do), Am. Bee J., 139. 51-60. 1999.
 
[4]  Salamanca Grosso, G; Henao Rojas, C; Moreno, I; Luna, A, “Características microbiológicas de las mieles tropicales de Apis mellifera” . Galeria Apícola virtual. Apiservices. 2001.
 
[5]  Hussein, M. H, “L’apiculture en Afrique: les pays du Nord, de l’Est, du Nord-Est et de l’ouest du continent,” Apicta, (1). 34-48. 2001.
 
[6]  Bogdanov, S., Martin, P., Lullmann, C., Borneck, R., Flamini, C., et al., “Harmonised methods of the European honey commission”, Apidologie,. (Extra issue). 1-59. 1997.
 
[7]  International Honey Commission (IHC), “Harmonized methods of the international Honey commission,” 1-63. 2009. Available: http://www.beehexagon.net/en/network.htm [accessed June, 2, 2017)].
 
[8]  Ward, W.H., Truman, K.F, “A quality survey of Australian Honeys,” RIRDC Publishers, 2001, 41.
 
[9]  Guillaume, P, “Milieu de culture PCA, milieu de culture GN,” 1-54. 2004. Available: www.ac-lyon.fr/enseigne/biotech/microbio. [Accessed Dec. 13, 2012].
 
[10]  Guiraud, J.P., Rosec, J.P, “Pratique des normes en microbiologie alimentaire,” ed. AFNOR.1-96. 2004.
 
[11]  Larpent-Gourgaud, M., Larpent, J.P, “ Mémento technique de microbiologie”, Lavoisier, Tec et Doc., 1997, 280.
 
[12]  Guiraud, J.P, “Microbiologie alimentaire,” Dunod, Paris, 2003, 651.
 
[13]  NF ISO 4832 (V 08-015), “Microbiologie des aliments. Méthode horizontale pour le dénombrement des coliformes. Méthode par comptage des colonies,” Jul. 2006.
 
[14]  NF V08-050, “Microbiologie des aliments. Dénombrement des coliformes présumés par comptage des colonies obtenues à 30°C,” Apr. 2009.
 
[15]  NF V08-060,“Microbiologie des aliments. Dénombrement des coliformes thermotolérants par comptage des colonies obtenues à 44°C,” Apr. 2009.
 
[16]  Petransxiene, D., Lapied, L., “La qualité bactériologique du lait et des produits laitiers, analyses et tests,” 2eme édition Technique et Documentation, Lavoisier, Paris, 1981, 228.
 
[17]  Larpent, J.P., Guiraud, L.M., “Manuel pratique de microbiologie,” Edition Hermann, 1985.
 
[18]  Moreau M, “Les moisissures,” In “microbiologie alimentaire,” Tome 2. Edition Tec et Doc, Paris, 1996, 54-62.
 
[19]  Martins, H.M., Martins, M.L., Bernardo, F.M.A, “Bacillaceae spores, fungi and aflatoxins determination in honey,” Revista Portuguesa De Ciências Vet- erinárias, 98(546). 85-8. 2003.
 
[20]  Boeufrag, J.M., Bonaly R., Larpent, J.P, “L’identification des levures,” In “Biotechnologie des levures,” Ed masson, 1991, 171-204.
 
[21]  Koenig H, “Guide de mycologie médicale,” Ellipse édition Marqueting Paris, 1995, 288.
 
[22]  Bouix, M., Leveau, J .V, “Les levures,” in technique d’analyses et de contrôle dans les industries agroalimentaire,” Edition technique et documentation, paris, volume 03, 1980, 130-143.
 
[23]  Bouix, M., Leveau, J. V, “Microbiologie industrielle,” Tome 01, Edition Lavoisier technique et documentation, Paris, 1993, 5-32.
 
[24]  Midgley, G., Yvonn, E., Clayton, M., Roderick, J. H, “Atlas de poche de mycologie”. Edition Flammarion, Paris, 1998, 15.
 
[25]  Bruneau, E, “Les analyses du miel: les paramètres physico-chimiques,” ActuAPI ; CARI, 2005, 1-8.
 
[26]  CETAM-LORRAINE, “Information sur des différentes analyses des miels,” Laboratoire d’analyses et d’écologie apicole. 2002, 1-5.
 
[27]  Mbogning, E., Tchoumboue, j., Damesse, F., Sanou Sobze, M., & Canini, A, “Caractéristiques physico- chimiques des miels de la zone Soudano-guinéenne de l’Ouest et de l’Adamaoua Cameroun,” Tropicultura, 29 (3). 168-175. 2011.
 
[28]  Omafuvbe, B. O., and Akanbi O. O, “Microbiological and physico-chemical properties of some commercial Nigerian honey,” African Journal of Microbiology Research Vol. 3 (12). 891-896. Dec. 2009.
 
[29]  Adebiyi, F.M., Akpan, I., Obiajunwa E.I., and Olaniyi, H.B, “Chemical/ Physical Characterization of Nigerian Honey,” Pakistan Journal of Nutrition, 3 (5). 278-281. 2004.
 
[30]  Meda, A., Lamien , C.E., Millogo,J., Romito , M., Nacoulma, O.G, “Physicochemical Analyses of Burkina Fasan Honey,” ACTA VET. BRNO, 74. 147-152. 2005.
 
[31]  Djossou, J.A., Tchobo, F.P., Yédomonhan, H., Alitonou, A.G., & Soumanou M, “Evaluation des caractéristiques physico-chimiques des miels commercialisés à Cotonou,” Tropicultura, 31 (3). 163-169. 2013.
 
[32]  Journal officiel des Communautés européennes (JOCE) . 12.1.2002 N°10, 47-52. ( The Council of the European Union. Council Directive 2001/110/ec of 20 Dec. 2001 relating to honey), Dec. 1, 2002.
 
[33]  Codex Alimentarius Commission Codex standard 12, Revised Codex Standard for Honey, Standards and Standard Methods. FAO- Rome, 2001, 1-7. Available: http://www. codex alimentarius. netCodex alimentaire (2001).
 
[34]  Mendes, E., Brojo, P.E., Ferreira, I., Ferreira, M.A, “Quality evaluation of Portuguese honey,” Carbohydr. Polym, 37(3). 219-223. 1998.
 
[35]  Terrab, A., Diez, M.J., Heredia, F.J, “Characterization of Moroccan unifloral honeys by their physicochemical characteristics,” Food Chem, 79. 373-379. 2002.
 
[36]  Gonnet, M, “L'hydroxyméthylfurfural dans les miels. Mise au point d'une méthode de dosage,” Ann. Abeille, 6 (1). 53-67. 1963.
 
[37]  Ruoff, K., Bogdanov, S, “Authenticity of honey and other bee products”. Apiacta, 38. 317-327. 2004.
 
[38]  Gonnet, M, “Le miel, composition, propriétés, conservation”. INRA station expérimentale d’apiculture, 1982, 1-18.
 
[39]  Schweitzer, P, “Qualité du miel,” Journée de l'abeille à Sombernon. Apiservices, Oct. 7, 2000.
 
[40]  Rabeharifara, Z.P, “Caractérisation alimentaire des miels malgaches en vue d’une authentification: cas des miels d’eucalyptus,” Mém DEA en Biochimie, Univ d’antananarivo, Madagascar. 2011, 102.
 
[41]  Gomes, S., Dias, L. G., Moreira, L.L., Rodrigues, P., Estevinho, L, “Physicochemical, microbiological and antimicrobial properties of commercial honeys from Portugal,” Food and Chemical Toxicology, Volume 48, Issue 2. 544-548. February 2010.
 
[42]  Bogdanov, S., Lüllmann, C., Martin, P., von der Ohe, W., Russmann, H., Vorwohl, G., Persano Oddo, L., et al. “Honey Quality and International Regulatory Standards: Review of the Work of the International Honey Commission,” Swiss Bee Research Centre, 1999, 7.
 
[43]  Louveaux, J, “Composition, propriétés et technologie du miel”, In traité de biologie de l’abeille, Tome 3: les produits de la ruche, Sect l’abeille et la fleur, Ed. Masson et CIE, Paris. 1968, 277-318.
 
[44]  Bogdanov, S., Rouff, K., Persano-Oddo, L,“Physico-chemical methods for the characterisation of unifloral honeys: a revieu,” Apidologie, (35). 4-17. 2004
 
[45]  Leblanc, P, “Univers merveilleux du miel: Caractères physicochimiques du miel,”. 2001, 61.
 
[46]  Donadieu, Y, “Le miel, thérapeutique naturelle,” 2ème édition Maloine, 1978, 14-27.
 
[47]  Louveaux, J, (1976). “Les produits de la ruche,” information technique des services vétérinaire, n°60 à 63. 67-78. 1976.
 
[48]  Malika, N., Faid, M., El adlouni, C, “Microbiological and physico-chemical properties of moroccan honey,” Int J Agri Biol, (7). 773-6. 2005.
 
[49]  Finola, M.S., Mirta, F., Lasango, C., Marioli, J.M, “Microbiological and chemical characterization of honeys from central Argentina,” Food Chemistry, 100.1649-953. 2007.
 
[50]  Codex alimentarius Commission, “Programme mixte FAO/OMS. Projet de norme codex révisée pour le miel”. CX/S 00/3 Novembre 1999.
 
[51]  C.R.C, ch. 287, “Règlement sur le miel”. 27 octobre (2015). Publié par le ministre canadien de la Justice. Available: http://lois-laws.justice.gc.ca [Accessed Aug. 15, 2017].
 
[52]  Fléché, C., Clément, M.C., Zeggane, S., & Faucon, J.P, “Contamination des produits de la ruche et risques pour la santé humaine: situation en France,” Rev. sci. tech. Off. int. Epiz., 16 (2). 609-619. 1997.
 
[53]  Hocine, L, “Caractérisation microbiologiques et physicochimiques des miels produits au niveau de la région de Tiaret,” PhD thesis in Sciences. Ibn khaldoun-Tiaret University-Algeria, 2017.
 
[54]  Hocine, L., and Rezzoug, W, “Microbiological and Physicochemical Characterization of Honeys from the Tiaret Region of Algeria,” Asian Journal of Pharmaceutical Research and Health Care, Vol 9(3). 85-91. 2017.
 
[55]  Iurlina, M. O., Fritz, R, “Characterization of microorganisms in Argentinean honeys from different sources,” International Journal of Food Microbiology, 105. 297-304. 2005.
 
[56]  Kunová, S., Kačániová, M., Haščík, P., Čuboň, J, “Microbiological and chemical quality of slovak and european honey,” J Microbiol Biotech Food Sci., 4 (special issue 1). 41-44. 2015.
 
[57]  Różańska, H, “Microbiological quality of polish honey,” Bull Vet Inst Pulawy, 55. 443-445. 2011.
 
[58]  Rall, V.L.M., Bombo, A.J., Lopes, T.F., Carvalho, L.R., Silva, M.G, “Honey consumption in the state of Sao Paulo:a risk to human health,” Anaerobe, (9). 299-303. 2003.
 
[59]  Sodré, G.S., Marchini, L.C., Moreti, A.C.C.C., Rosa, V.P., Carvalho, C.A.L, “Conteúdo microbiológico de méis de Apis mellifera (Hymenoptera: Apidae) dos Estados do Ceará e Piauí,” Boletim de Indústria Animal, 64. 39-42. 2007.
 
[60]  Ratia, G, “Cahier des charges concernant le mode de production biologique du miel,” Bureau des signes de qualité et de l’apiculture biologique, France. 2001. 13.
 
[61]  Kitambala, K, “Etude bactériologique et biochimique du miel vendu au marché central de Bukavu (Congo),” Tropicultura, 16-17 (4). 189-192.1999.
 
[62]  Snowdon, J.A., Cliver, D.O, “Microorganisms in honey,”. Int. J. Food Microbiol. 31. 1-6. 1996.
 
[63]  Jouve, L. J, (1996). La qualité microbiologique des aliments maîtrise et critères, 2eme édition; Ed. Polytechnica, Paris, 1996. 186-354.
 
[64]  Dromigny, E, Les critères microbiologiques des denrées alimentaires, Ed tec & doc lavoisier, 2012. 509.
 
[65]  ANSES, “Bacillus cereus,” Fiche de description de danger biologique transmissible par les aliments, Septembre 2011.
 
[66]  Collins, C.H., Lyne, P.M., Grange, J.M, Collins and Lyne’s microbiological methods. 7th ed. Oxford: Butterworth-Heinemann, 1999, 213-221.
 
[67]  Gliński, Z., Chmielewski, M, “Pathology and therapy of diseases of usable insects,” Agricultural Academy Press, Lublin, Poland. 1994.
 
[68]  Kačaniová, M., Chlebo, R., Kopernický, M., Trakovická, A, “Microflora of the honeybee gastrointestinal tract,” Folia Microbiol, 2004 (49). 169-171.
 
[69]  Nasser Laila, A, (2004). “Isolation and characterization of fungi contaminating packaged honey commonly consumed in saudi Arabia,” Ass. Univ. Bull. Environ. Res. Vol. 7 No. 1, 2004, March 1-7.
 
[70]  Root, A.I. (Ed.), “The ABC and XYZ of Bee Culture,” The A. I. Root Co., Medina, OH. 1983.
 
[71]  Graham, J.M. (Ed.), (1992). The Hive and the Honey Bee, Dadant and Sons, Hamilton, Illinois. 1992.3330.
 
[72]  Coll Cárdenas, F., Villat, C., Laporte, G., Noia, M., Mestorino, N, “Microbial characteristic of honey,” A Review. Veterinaria Cuyana Año 3, 2008, nº 1 y 2.
 
[73]  Moreau, C, “Les moisissures,” in microbiologie alimentaire. Tome I, Edition Technique & documentation, Paris. 1996. 237-247.