Protective Effects of Seabuckthorn Seed Oil against Acute Alcoholic-induced Liver Injury in Rats

Meihong Xu¹, Na Wang², Weirui Zhang² and Junbo Wang^1,

¹Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing, PR China

²R&D center of TIENS Ltd., Tianjin, PR China

Cite this paper:
Meihong Xu, Na Wang, Weirui Zhang and Junbo Wang. Protective Effects of Seabuckthorn Seed Oil against Acute Alcoholic-induced Liver Injury in Rats. Journal of Food and Nutrition Research. 2014; 2(12):1037-1041. doi: 10.12691/jfnr-2-12-27

Abstract

The present study examined the protective effects of seabuckthorn seed oil (Hippophae rhamnoidesL seed oil., SBT) on acute alcoholic-induced hepatic damage in male SD rats. Our results showed that oral administration of SBT seed oil at doses of 0.167, 0.333 and 0.500 g/kg for 45 days significantly reduced the changed levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum, and the level of triglyceride (TG) and malondialdehyde (MDA) in liver, which were induced by alcoholic-induced in rats. Moreover, the treatment of SBT seed oil was also found to significantly increase the activities of sglutathione peroxidase (GSH-Px) in liver. Our study found that the optimal dose of SBT seed oil was 0.333 g/kg, as the minimum amount exhibiting the greatest hepatoprotective effects on alcoholic-induced liver injury, which have been supported by the evaluation of the liver histopathology in rats. In conclusion, it has been found the hepatoprotective effect of SBT seed oil at all tested doses.

Keywords:
seabuckthorn seed oil alcoholic-induced liver antioxidation

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]	Shi, H.L., Cai, H.J., Chen, X.Y., Yang, C.M. Studies on the antioxidative effects of Hippophae rhamnoidesL. seed oil. Acta Nutr. Sin, Vol. 1994; 3: 292-5 (in Chinese).
[2]	Ganju, L., Padwad, Y., Singh, R., Karan, D., Chanda, S., Chopra, M.K., Bhatnagar, P., Kashyap, R., Sawhney, R.C. Anti-inflammatory activity of seabuckthorn (Hippophae rhamnoides) leaves. Int. Immunopharmacol. 2005; 5(12): 1675-84.
[3]	Geetha, S., Jayamurthy, P., Pal, K., Pandey, S., Kumar, R., Sawhney, R.C. Hepatoprotective effects of seabuckthorn (Hippophae rhamnoidesL.) against carbon tetrachloride induced liver injury in rats. J. Sci. Food Agric. 2008; 88(9): 1592-97.
[4]	Pintea, A., Marpeau, A., Faye, M., Socaciu, C., Gleizes, M. Polar lipid and fatty acid distribution in carotenolipoprotein complexes extracted from seabuckthorn fruits. Phytochem. Anal. 2001; 12(5): 293-8.
[5]	Kallio, H., Yang, B., Peippo, P. Effects of different origins and harvesting time on vitamin C, tocopherols and tocotrienols in seabuckthorn (Hippophae rhamnoides) berries. J. Agric. Food Chem, 2002a; 50(21): 6136-42.
[6]	Yang, B., Kallio, H. Composition and physiological effects of seabuckthorn (Hippophae) lipids. Trends Food. Sci. Tech. 2002; 13: 160-7.
[7]	Yang, B., Karlsson, R., Oksman, P., Kallio, H. Phytosterols in seabuckthorn (Hippophae rhamnoidesL.) berries: identification and effects of different origins and harvesting times. J. Agric. Food Chem. 2001; 49(11): 5620-9.
[8]	Kallio, H., Yang, B., Peippo, P., Tahvonen, R., Pan, R. Triacylglycerols, glycerophospholipids, tocopherols, and tocotrienols in berries and seeds of two subspecies (ssp. sinensis and ssp. mongolica) of seabuckthorn (Hippophae rhamnoides). J. Agric. Food Chem. 2002b; 50(10): 3004-9.
[9]	Negi, P.S., Chauhan, A.S., Sadia, G.A., Rohinishree, Y.S., Ramteke, R.S. Antioxidant and antibacterial activities of various seabuckthorn (Hippophae rhamnoidesL.) seed extracts. Food Chem. 2005; 92: 119-24.
[10]	Basu, M., Prasad, R., Jayamurthy, P., Pal, K., Arumughan, C., Sawhney, R.C. Anti-atherogenic effects of seabuckthorn (Hippophaea rhamnoides) seed oil. Phytomedicine. 2007; 14(11): 770-7.
[11]	Berton, T.R., Conti, C.J., Mitchell, D., Cheng, T.J., Wang, Y.B., Gao, L.P., Sun, Y.F., Zhang, J. The protection of seed oil ofHippophae rharmnoideson ischemic cerebral infarction in rats”, Zhongguo Zhong Yao Za Zhi, 2003; 28: 548-50 (in Chinese).
[12]	Purushothaman, J., Suryakumar, G., Shukla, D., Malhotra, A.S., Kasiganesan, H., Kumar, R., Sawhney, R.C., Chami, A. Modulatory effects of seabuckthorn (Hippophae rhamnoidesL.) in hypobaric hypoxia induced cerebral vascular injury. Brain Res. Bull. 2008; 77(5): 246-52.
[13]	Hsu, Y.W., Tsai, C.F., Chen, W.K., Lu, F. J. Protective effects of seabuckthorn (Hippophae rhamnoides L.) seed oil against carbon tetrachloride-induced hepatotoxicity in mice. Food and Chemical Toxicology 2009; 47(9): 2281-8.
[14]	Cheng, T.J., Pu, J.K., Wu, L.W., Ma, Z.R., Cao, Z., Li, T.J. An preliminary study on hepato-protective action of seed oil ofHippophae rhamnoidesL. (HR) and mechanism of the action. Zhongguo Zhong Yao Za Zhi. 1994; 19: 367-70 (in Chinese).