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Journal of Food and Nutrition Research. 2014, 2(12), 880-889
DOI: 10.12691/jfnr-2-12-4
Open AccessArticle

Study on Neuroprotective Capacity of Key Flavonoids of Ampelopsis grossedentata

Jiasheng Wang1, Zhengwen Yu1, , Qiuping Yu1, Qi Kong1, Ni Zhang1, Leilei He1 and Yin Yi1

1School of Life Sciences, Guizhou Normal University, Guiyang, P. R. China

Pub. Date: November 05, 2014
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Cite this paper:
Jiasheng Wang, Zhengwen Yu, Qiuping Yu, Qi Kong, Ni Zhang, Leilei He and Yin Yi. Study on Neuroprotective Capacity of Key Flavonoids of Ampelopsis grossedentata. Journal of Food and Nutrition Research. 2014; 2(12):880-889. doi: 10.12691/jfnr-2-12-4

Abstract

Ampelopsis grossedentata could be of potential significance for health food applications in view of the abundant flavonoids, most of which could be effective in preventing and curing senile diseases. To evaluate the neuroprotective effects of flavonoids in A. grossedentata, model of Aβ25-35-induced PC12 cells apoptosis was established, and methods as MTT assay for determining cell viability, DCFH-DA fluorescence analysis for the detection of intracellular reactive oxygen species (ROS) induced by Aβ25-35, and the DPPH radical scavenging assay for evaluating the antioxidant activity were applied. Cells co-incubated with flavonoids (100 μM) and Aβ25-35 showed a significantly higher survival rate (P<0.01 or P<0.05) than with Aβ25-35 only, flavonoids in particular kaempferol, rutin, kaempferol-3-O-β-D-glucopyranoside and isoquercitrin exerted better neuroprotective effects than the positive control donepezil HCl. ROS induced by Aβ25-35 in PC12 cells decreased after exposure to 100 μM flavonoids and extracts, which inosculated with the data of DPPH assay. In the way of inhibiting ROS production, flavonoids in A. grossedentata could attenuate cellular apoptosis induced by Aβ25-35, and it is concluded that when administered in soluble preparation, A. grossedentata being rich in flavonoids that structurally related to dihydromyricetin could become a mine for the development of a new generation of food to be clinically effective in dementia.

Keywords:
flavonoids ampelopsis grossedentata Aβ25-35 PC12 cells neuroprotective capacity ROS

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  Kounnas, M.Z., Danks, A.M., Cheng, S., Tyree, C., Ackerman, E., Zhang, X., Ahn, K., Nguyen, P., Comer, D., Mao, L., Yu, C., Pleynet, D., Digregorio, P.J., Velicelebi, G., Stauderman, K.A., Comer, W.T., Mobley, W. C., Li, Y.M., Sisodia, S.S., Tanzi, R.E. and Wagner, S.L, “Modulation of gamma-secretase reduces beta-amyloid deposition in a transgenic mouse model of Alzheimer’s disease,” Neuron, 67 (5). 769-780. Sep. 2010.
 
[2]  Lesné, S., Koh, M.T., Kotilinek, L., Kayed, R., Glabe, C.G., Yang, A., Gallagher, M. and Ashe, K.H, “A specific amyloid-beta protein assembly in the brain impairs memory,” Nature, 440 (7082). 352-357. Mar. 2006.
 
[3]  Naldi, M., Fiori, J., Pistolozzi, M., Drake, A.F., Bertucci, C., Wu, R.L., Mlynarczyk, K., Filipek, S., De Simone, A. and Andrisano, V, “Amyloid β-peptide 25-35 self-assembly and its inhibition: a model undecapeptide system to gain atomistic and secondary structure details of the Alzheimer's disease process and treatment,” ACS Chemical Neuroscience, 3 (11). 952-962. Sep. 2012.
 
[4]  Reitz, C, “Alzheimer's Disease and the Amyloid Cascade Hypothesis: A Critical Review,” International Journal of Alzheimer's Disease, 2012. 369808. 2012. [Online]. Available: http://www.hindawi.com/journals/ijad/2012/369808/. [Accessed Jul. 28, 2014].
 
[5]  P. Giannakopoulos and P.R. Hof, Dementia in Clinical Practice, Karger Medical and Scientific Publishers, 2009. [E-book] Available: http://www.karger.com/Article/FullText/197887.
 
[6]  Goedert, M. and Spillantini, M.G, “A century of Alzheimer’s disease,” Science, 314 (5800). 777-781. Nov.2006.
 
[7]  Wang, X., Kim, J.R., Lee, S.B., Kim, Y.J., Jung, M.Y., Kwon, H.W. and Ahn, Y.J, “Effects of curcuminoids identified in rhizomes of Curcuma longa on BACE-1 inhibitory and behavioral activity and lifespan of Alzheimer's disease Drosophila models,” BMC Complementary and Alternative Medicine, 14 (1). 88. Mar. 2014.
 
[8]  Awale, S., Tohda, C., Tezuka, Y., Miyazaki, M. and Kadota, S, “Protective Effects of Rosa damascena and Its Active Constituent on Aβ (25-35)-Induced Neuritic Atrophy,” Evidence-based complementary and alternative medicine, 2011. 131042. Aug. 2011. [Online]. Available: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3162985/. [Accessed Jul. 26, 2014].
 
[9]  Giovanna, C., Cecchi, C., Pensalfini, A., Bonini, S.A., Ferrari-Toninelli, G., Liguri, G., Memo, M. and Uberti, D, “Generation of reactive oxygen species by beta amyloid fibrils and oligomers involves different intra/extracellular pathways,” Amino Acids, 38 (4). 1107. Apr. 2010.
 
[10]  Wei, G. andShea, J.E, “Effects of solvent on the structure of the Alzheimer amyloid-β (25-35) peptide,” Biophysical Journal, 91 (5). 1638-1647. Sep. 2006.
 
[11]  Gan, C.S., Zhao, Z.Z., Nan, D.D., Yin, B.B. and Hu, J.Y, “Homoisoflavonoids as potential imaging agents for b-amyloid plaques in Alzheimer’s disease,” European Journal of Medicinal Chemistry, 76. 125-131. Apr. 2014.
 
[12]  Ansari, N. and Khodagholi F, “Natural products as promising drug candidates for the treatment of Alzheimer's disease: molecular mechanism aspect,” Current Neuropharmacology, 11 (14). 414-429. Jul. 2013.
 
[13]  Liu, R., Zhang, T.T., Yang, H.G., Lan, X., Ying, J. and Du, G.H, “The Flavonoid Apigenin Protects Brain Neurovascular Coupling against Amyloid-25-35-Induced Toxicity in Mice,” Journal of Alzheimer’s Disease, 24 (1). 85-100. Feb. 2011.
 
[14]  Klementiev, B., Novikova, T., Novitskaya, V., Walmod, P.S., Dmytriyeva, O., Pakkenberg, B., Berezin, V. and Bock, E, “A neural cell adhesion molecule-derived peptide reduces neuropathological signs and cognitive impairment induced by Aβ25-35,” Neuroscience, 145 (1). 209-224. Mar. 2007.
 
[15]  Kuboyama, T., Tohda, C. and Komatsu, K, “Neuritic regeneration and synaptic reconstruction induced by withanolide A,” British Journal of Pharmacology, 144 (7). 961-971. Apr. 2005
 
[16]  Hong, S.Y., Jeong, W.S. and Jun, M, “Protective Effects of the Key Compounds Isolated from Cornifructus against β-Amyloid-Induced Neurotoxicity in PC12 Cells,” Molecules, 17. 10831-10845. Sep. 2012.
 
[17]  Kim, M.J., Lee, J. and Seong, A.R, “Neuroprotective effects of Eriobotrya japonica against beta-amyloid-induced oxidative stress and memory impairment,” Food and chemical toxicology, 49(4). 780-784. Dec. 2011.
 
[18]  Muthaiyah, B., Essa, M.M., Chauhan, V. and Chauhan, A, “Protective effects of walnut extract against amyloid beta peptide-induced cell death and oxidative stress in PC12 cells,” Neurochemical Research, 36 (11). 2096-2103. Nov. 2011.
 
[19]  Agostinho, P., Cunha, R.A. and Oliveira, C, “Neuroinflammation, oxidative stress and the pathogenesis of Alzheimers disease,” Current Pharmaceutical Design, 16 (25). 2766-2278. 2010.
 
[20]  Jung, H.A., Jin, S.E., Choi, R.J., Kim, D.H., Kim, Y.S., Ryu, J.H., Kim, D.W., Son, Y.K., Park, J.J. and Choi, J.S, “Anti-amnesic activity of neferine with antioxidant and anti-inflammatory capacities, as well as inhibition of ChEs and BACE1,” Life Sciences, 87 (13-14). 420-430. Sep. 2010.
 
[21]  Orhan, I. and Aslan, M, “Appraisal of scopolamine-induced antiamnesic effect in mice and in vitro antiacetylcholinesterase and antioxidant activities of some traditionally used Lamiaceae plants,” Journal of Ethnopharmacology, 122 (2). 327-332. Mar. 2009.
 
[22]  Wang, Z.H., Kang, K.A., Zhang, R., Piao, M.J., Jo, S.H., Kim, J.S., Kang, S.S., Lee, J.S., Park, D.H. and Hyun, J.W, “Myricetin suppresses oxidative stress-induced cell damage via both direct and indirect antioxidant action,” Environmental Toxicology and Pharmacology, 29 (1). 12-18. Jan. 2010.
 
[23]  Ge, Y.S., Teng, W.Y. and Zhang, C.D, “Protective effect of cyclophilin A against Alzheimer’s amyloid beta-peptide (25-35)-induced oxidative stress in PC12 cells,” Chinese medical journal, 122 (6). 716-724. Mar. 2009.
 
[24]  Cassagnes, L.-E., Herve, V., Nepveu, F., Hureau, C., Faller, P. and Collin, F, “The Catalytically Active Copper-Amyloid-Beta State: Coordination Site Responsible for Reactive Oxygen Species Production,” AngewandteChemie International Edition, 52 (42). 11110-11113. Oct. 2013.
 
[25]  Ahn, B.R., Moon, H.E., Kim, H.R., Jung, H.A. and Choi, J.S, “Neuroprotective Effect of Edible Brown Alga Eiseniabicyclis on Amyloid Beta Peptide-induced Toxicity in PC12 Cells,” Archives of Pharmacal Research, 35 (11). 1989-1998. Nov. 2012.
 
[26]  Jakob-Roetne, R. and Jacobsen, H, “Alzheimer’s disease: from pathology to therapeutic approaches,” Angewandte Chemie International Edition, 48 (17). 3030-3059. Apr. 2009.
 
[27]  Yang, S.G., Wang, W.Y., Ling, T.J., Feng, Y., Du, X.T., Zhang, X., Sun, X.X., Zhao, M., Xue, D., Yang, Y. and Liu, R.T, “Alpha-tocopherolquinone inhibits beta-amyloid aggregation and cytotoxicity, disaggregates preformed fibrils and decreases the production of reactive oxygen species, NO and inflammatory cytokines,” Neurochemistry International, 57 (8). 914-922. Dec. 2010.
 
[28]  Orhan, I.E, “Current concepts on selected plant secondary metabolites with promising inhibitory effects against enzymes linked to Alzheimer’s disease,” Current Medicinal Chemistry, 19 (14). 2252-2261. May. 2012.
 
[29]  Mandel, S.A., Amit, T., Weinreb, O., Reznichenko, L. and Youdim, M.B, “Simultaneous manipulation of multiple brain targets by green tea catechins: a potential neuroprotective strategy for Alzheimer and Parkinson diseases,” CNS Neuroscience & Therapeutics, 14 (4). 352-365. Win. 2008.
 
[30]  Ribeiro, D., Freitas, M., Tome, S.M., Silva, A.M.S., Porto, G. and Fernandes, E, “Modulation of human neutrophils' oxidative burst by flavonoids,” European Journal of Medicinal Chemistry, 67. 280-292. Sep. 2013.
 
[31]  Mendes, A.P., Borges, R.S., Neto, A.M., de Macedo, L.G. and da Silva, A.B, “The basic antioxidant structure for flavonoid derivatives,” Journal of Molecular Modeling, 18(9). 4073-4080. Sep. 2012.
 
[32]  Prochazkova, D., Bousova, I. and Wilhelmova, N, “Antioxidant and prooxidant properties of flavonoids,” Fitoterapia, 82 (4). 51 3-523. Jan. 2011.
 
[33]  Zhang, R., Kang, K.A., Piao, M.J., Ko, D.O.,Wang, Z.H., Chang,W.Y., You, H.J., Lee, I.K., Kim, B.J., Kang, S.S. and Hyun, J.W, “Preventive effect of 7,8-dihydroxyflavone against oxidative stress induced genotoxicity,” Biological & pharmaceutical bulletin, 32. 166-171. Feb. 2009.
 
[34]  Piao, M.J., Kang, K.A., Zhang, R., Ko, D.O., Wang, Z.H., You, H.J., Kim, H.S., Kim, J.S., Kang, S.S. and Hyun, J.W, “Hyperoside prevents oxidative damage induced by hydrogen peroxide in lung fibroblast cells via an antioxidant effect,” Biochimica et biophysicaacta(BBA)-General Subjects, 1780 (12). 1448-1457. Dec. 2008.
 
[35]  National Health and Family Planning Commission of the PRC, Notice on the approval of applying Ampelopsis grossedentata as a new food resource. NHFPC (2013) No. 16. [Online] Available: http://www.moh.gov.cn/sps/s7890/201401/bca788aead084936b9ef04f99ad5a2b8.shtml
 
[36]  Liu, B.G., Du, J.Q., Zeng, J., Chen, C.G. and Niu, S.Y, “Characterization and antioxidant activity of dihydromyricetin-lecithin complex,” European Food Research and Technology, 230 (2). 325-331. Dec. 2009.
 
[37]  Li, H., Li,Y., Zhang, Y., Shi, H., Hu, W. and Zhang, Z, “Comparison of Refluxing, Ultrasonic-and Microwave-Assisted Extraction of Dihydromyricetin from Ampelopsis grossedentata,” Journal of AOAC International, 91 (6). 1278-1283. Nov-Dec. 2008.
 
[38]  Zhang, Y., Que, S., Yang, X., Bin, W., Qiao, L. and Zhao Y, “Isolation and identification of metabolites from dihydromyricetin,” Magnetic Resonance in Chemistry, 45 (11). 909-916. Nov. 2007.
 
[39]  Chobot, V. and Hadacek, F, “Exploration of pro-oxidant and antioxidant activities of the flavonoid myricetin,” Redox Report, 16 (6). 242-247. Nov. 2011.
 
[40]  Faria, A.F., Marques, M.C. and Mercadante, A.Z, “Identification of bioactive compounds from jambolão (Syzygiumcumini) and antioxidant capacity evaluation in different pH conditions,” Food Chemistry, 126 (4). 1571-1578. Jun. 2011.
 
[41]  Rodríguez, A.T., Moreno, Y.S., Guadarrama, S.V. andTejacal, I.A. “Soluble phenols and antioxidant activity in mameysapote (Pouteriasapota) fruits in postharvest,” Food Research International, 44 (7). 1956-1961. Aug. 2011.
 
[42]  Xu, P.X., Wang, S.W., Yu, X.L., Su, Y.J., Wang, T., Zhou, W.W., Zhang, H., Wang, Y.J. and Liu, R.T, “Rutin improves spatial memory in Alzheimer's disease transgenic mice by reducing Aβ oligomer level and attenuating oxidative stress and neuroinflammation,” Behavioural Brain Research, 264 (1). 173-180. May. 2014.
 
[43]  Chen, L., Wang, C., Wei, Q.Y., Ning, Z.X. and Yuan, E.D, “Interaction of dihydromyricetin and alpha-amylase,” Natural Product Communications, 8 (3). 339-342. Mar. 2013.
 
[44]  Wang, S.W., Wang, Y.J., Su, Y.J., Zhou, W.W., Yang, S.G., Zhang, R., Zhao, M., Li, Y.N., Zhang, Z.P., Zhan, D.W. and Liu, R.T, “Rutin inhibits β-amyloid aggregation and cytotoxicity, attenuates oxidative stress, and decreases the production of nitric oxide and proinflammatory cytokines,” Neurotoxicology, 33 (3). 482-490. Jun. 2012.
 
[45]  Tedeschi, A., D’Errico, G., Lauro, M.R., Sansone, F., Di Marino, S., D’Ursi, A.M. and Aquino, R.P, “Effect of flavonoids on the Aβ (25-35)-phospholipid bilayers interaction,” European journal of medicinal chemistry, 45 (9). 3998-4003. Sep. 2010.
 
[46]  Kou, X.J., Shen, K.Y., An, Y.H., Qi, S.M., Dai, W.X. and Yin, Z.M, “Ampelopsin inhibits H2O2-induced apoptosis by ERK and Akt signaling pathways and up-regulation of heme oxygenase-1,” Phytotherapy Research, 26 (7). 988-994. Jul. 2012.
 
[47]  Detoma, A.S., Choi, J.S., Braymer, J.J. and Lim, M.H, “Myricetin: a naturally occurring regulator of metal-induced amyloid-β aggregation and neurotoxicity,” Chem Biochem, 12 (8). 1198-1201. May. 2011.
 
[48]  Shimmyo, Y., Kihara, T., Akaike, A., Niidome, T. and Sugimoto, H, “Multifunction of myricetin on A beta: neuroprotection via a conformational change of A beta and reduction of A beta via the interference of secretases,” Journal of Neuroscience Research, 86 (2). 368-377. Feb. 2008.
 
[49]  Shimmyo, Y., Kihara, T., Akaike, A., Niidome, T. and Sugimoto, H, “Three distinct neuroprotective functions of myricetin against glutamate-induced neuronal cell death: involvement of direct inhibition of caspase-3,” Journal of Neuroscience Research, 86 (8). 1836-1845. Jun. 2008.
 
[50]  Laabich, A., Manmoto, C.C., Kuksa, V., Leung, D.W., Vissvesvaran, G.P., Karliga, I., Kamat, M., Scott, I.L., Fawzi, A. and Kubota, R, “Protective effects of myricetin and related flavonols against A2E and light mediated-cell death in bovine retinal primary cell culture,” Experimental eye research, 85 (1). 154-165. Jul. 2007.
 
[51]  Greene, L.A. and Tischler, A.S, “Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor,” PNAS, 73 (7). 2424-2428. Jul. 1976.
 
[52]  Hansen, M.B., Nielsen, S.E. and Berg, K, “Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill,” Journal of immunological methods, 119. 203-210. May. 1989.
 
[53]  Denizot, F. and Lang, R, “Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability,” Journal of immunological methods, 89 (2). 271-277. May. 1986.
 
[54]  Sharma, Q.P. and Bhat, T.K, “DPPH antioxidant assay revisited,” Food Chemistry, 113 (4). 1202-1205. Apr. 2009.
 
[55]  Wang, H. and Joseph, J.A, “Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader,” Free Radical Biology and Medicine, 27 (5-6). 612-616. Sep. 1999.
 
[56]  Zhang, M.S., Zhang, N., He, L. L., Yu, Q. P., Yang, Z. N., Liu, S. M and Yu, Z.W, “Analysis of the content of dihydromyricetin and myricetin in Ampelosis grossedentata from Jiangkou,” Guangdong Agricultural Sciences, 15. 124-125, 139. Aug. 2012.
 
[57]  Wang, J.S., He, L. L., Zhang, N., Kong, Q., Yu, Q. P. and Yu, Z.W, “Dihydromyricetin in Ampelosis grossedentata collected from different habitats”, Chinese Traditional Patent Medicine, 36 (1). 145-147. Jan. 2014.
 
[58]  Yu,Q. P., He,L. L., Wang, J.S., Zhang,N., Kong, Q. and Yu, Z.W, “Dynamics Changes of Functional Ingredients from Cultivated Ampelopsis grossedentata,” China Journal of Experimental Traditional Medical Formulae, 20 (9). 66-70. May 2014.
 
[59]  Zhang, N., Wang, J.S., He, L. L., Yu, Q. P., Kong, Q. and Yu, Z.W, “Analysis on Fatty Acids of the Ampelosis grossedentata by GC-MS,” Seed, 32 (9). 17-18. Sep. 2013.
 
[60]  Hoi, C.P., Ho, Y.P., Baum, L. and Chow, A.H, “Neuroprotective effect of honokiol and magnolol, compounds from Magnolia officinalis, on beta-amyloid-induced toxicity in PC12 cells,” Phytotherapy Research, 24 (10). 1538-1542. Oct. 2010.
 
[61]  Jang, Y.J., Kang, N.J., Lee, K.W. and Lee, H.J, “Protective Effects of Red Wine Flavonols on 4-Hydroxynonenal-Induced Apoptosis in PC12 Cells,” in 2009 Annals of the New York Academy of Sciences, The New York Academy of Sciences, 170-175.
 
[62]  Cho, N., Choi, J.H., Yang, H.,Jeong, E.J., Lee, K.Y., Kim, Y.C. and Sung, S.H, “Neuroprotective and anti-inflammatory effects of flavonoids isolated from Rhusverniciflua in neuronal HT22 and microglial BV2 cell lines,” Food and Chemical Toxicology, 50.1940-1945. Jun. 2012.
 
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