Jiasheng Wang¹, Zhengwen Yu^1,, Qiuping Yu¹, Qi Kong¹, Ni Zhang¹, Leilei He¹, Yin Yi¹

Ampelopsis grossedentata could be of potential significance for health food applications in view of the abundant flavonoids, most of which could be effective in preventing and curing senile diseases. To evaluate the neuroprotective effects of flavonoids in A. grossedentata, model of Aβ_25-35-induced PC12 cells apoptosis was established, and methods as MTT assay for determining cell viability, DCFH-DA fluorescence analysis for the detection of intracellular reactive oxygen species (ROS) induced by Aβ_25-35, and the DPPH radical scavenging assay for evaluating the antioxidant activity were applied. Cells co-incubated with flavonoids (100 μM) and Aβ_25-35 showed a significantly higher survival rate (P<0.01 or P<0.05) than with Aβ_25-35 only, flavonoids in particular kaempferol, rutin, kaempferol-3-O-β-D-glucopyranoside and isoquercitrin exerted better neuroprotective effects than the positive control donepezil HCl. ROS induced by Aβ_25-35 in PC12 cells decreased after exposure to 100 μM flavonoids and extracts, which inosculated with the data of DPPH assay. In the way of inhibiting ROS production, flavonoids in A. grossedentata could attenuate cellular apoptosis induced by Aβ_25-35, and it is concluded that when administered in soluble preparation, A. grossedentata being rich in flavonoids that structurally related to dihydromyricetin could become a mine for the development of a new generation of food to be clinically effective in dementia.

flavonoids, ampelopsis grossedentata, Aβ_25-35, PC12 cells, neuroprotective capacity, ROS