The Comparison and Analysis of Three Extraction Methods for Polysaccharides in Purslane

Chunmei GU^1, and Shu PAN¹

¹Institute of Food Science and Engineering, Jilin Agricultural University, Changchun, China

Cite this paper:
Chunmei GU and Shu PAN. The Comparison and Analysis of Three Extraction Methods for Polysaccharides in Purslane. Journal of Food and Nutrition Research. 2014; 2(7):401-405. doi: 10.12691/jfnr-2-7-12

Abstract

In this study, three extraction methods of polysaccharides in Purslane were compared and analyzed, and the best method was ascertained. Purslane polysaccharides were extracted using water, ultrasonic and microwave. By the results of single factor experiments and orthogonal experiments, the optimal extracting conditions were selected and then the best extraction method was determined. In order to calculate the extraction yield of polysaccharides, its content were measured by the phenol–sulfuric acid method using glucose as the standard, and then the efficiency of three extraction methods was evaluated. The results showed that the extraction yield of ultrasonic was 35.42% under the optimal conditions of 60C, 60 min, 1:20 and 200w. The extraction yield of microwave method was 32.12% under the optimal conditions of 3 min, 1:25 and 500w. The extraction yield of water method was 31.22% under the optimal conditions of 80C, 2 h, 1:30 and 3 times. It could be concluded that ultrasonic method was the best one in these three methods.

Keywords:
Purslane polysaccharides Extraction methods comparison analysis

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References:

[1]	Lim, Y. Y. and Quah, E. P. L., “Antioxidant properties of different cultivars of Portulaca oleracea,” Food Chemistry, 103 734-740. 2007
[2]	Chan, K., Islam, M. W., Kamil, M., Radhakrishnan, R., Zakaria, M. N. and Habibullah, M., “The analgesic and anti-inflammatory effects of Portulaca oleracea L. subsp. Sativa (Haw.) Celak,” Journal of Ethnopharmacology, 73. 445-451. 2000.
[3]	Dkhil, M. A., Abdel Moniem, A. E., Al-Quraishy, S. and Saleh, R. A., “Antioxidant effect of purslane (Portulaca oleracea) and its mechanism of action,” Journal of Medicinal Plants Research, 5. 1563-1589. 2011.
[4]	Rashed, A. N., Afifi, F. U. and Disi, A. M., “Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculus JVI-1,” Journal of Ethnopharmacology, 88. 131-136. 2003.
[5]	Yang, S.C. and Guo, J.F., “Purslane nutrition health care function and its development and utilization, ”The food and nutrition of China, 5. 21-23. 2004.
[6]	Chen, C.J., Wang, W.Y., Wang, X.L, Dong, L.W., Yue, Y.T., Xin, H.L., Ling, C.Q. and Li, M., “Anti-hypoxic activity of the ethanol extract from Portulaca oleracea in mice,” Journal of Ethnopharmacology, 124. 246-250. 2009
[7]	Niu, G.C., “Progress on chemical compositions and pharmacological function of Portulace oleracea L,” Journal of Anhui Agriculture Science, 33, 1090-1092. 2005.
[8]	Samy, J., Sugumaran, M. and Lee, K. L. W., “Herbs of Malaysia: An Introduction to the medicinal, culinary, aromatic and cosmetic use of herbs,” Kuala Lumpur: Times Edition. 2004.
[9]	Ghazanfar, S.A. “Handbook of Arabian Medicinal Plants,” CRC Press, Boca Raton, FL,p. 176. 1994.
[10]	Okwuasaba, F., Ejike, C. and Parry, O., “Skeletal muscle relaxant properties of the aqueous extract of Portulaca oleracea,” Journal of Ethnopharmacology, 17.139-160. 1986.
[11]	Okwuasaba, F., Parry, O. and Ejike, C., “Investigation into the mechanism of action of extracts of Portulaca oleracea,” Journal of Ethnopharmacology, 21. 91-97. 1987.
[12]	Parry, O., Okwuasaba, F. and Eijike, C., “Effect of an aqueous extract of Portulaca olearcea leaves on smoothmuscle and rat blood pressure,” Journal of Ethnopharmacology, 22. 33-44. 1988.
[13]	Parry, O., Marks, J.A. and Okwuasaba, F.K., “The skeletal muscle relaxant action of Portulaca oleracea: role of potassium ions,” Journal of Ethnopharmacology, 40. 187-194. 1993.
[14]	Xie, J.H., Shen, M.Y., Nie, S.P., Zhao, Q., Li, C. and Xie, M.Y., “Separation of water-soluble polysaccharides from Cyclocarya paliurusby ultrafiltration process,” Carbohydrate Polymers, 101. 479-483. 2014.
[15]	Zhou, J., Liu, J.W., Fu, J.W. and Hao, L.F., “Purslane polysaccharide extraction and content determination,” Chinese herbal medicine, 6. 55-57. 2001.
[16]	Li. Y.P., Ye.J., Su.H., Wang, D.J. and Zhou, C.L., “The research progress of purslanepolysaccharide,” Food science, 28. 538-540. 2007.
[17]	Gao, L., Liu, J., Tian, Y.J. and Hao, Y.J., “The preliminary research on the purslane polysaccharide extraction and purification process,” Food research and development, 27. 59-62. 2006.
[18]	Qu, X.L., Gao, H.L., Duan, R. and Su, X.H., “The method of ultrasonic extraction of purslane polysaccharides research,” Journal of taishan medical college, 2. 119-121. 2009.
[19]	Qian, Z.W. and Bi, S.M., “Microwave assisted extraction of purslane polysaccharides research,” Guangdong agricultural science, 37, 149-150. 2010.
[20]	Zhang, H.Y. and Zheng, L., “The extraction of purslane polysaccharide and antibacterial activity in vitro,” Jiangsu agricultural science, 39. 413-415. 2011.
[21]	Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F., “Colorimetric method for determination of sugars and related substances,” Analytical Chemistry, 28 (3). 350-356. 1956.
[22]	Mohammad, T. B., Mohammad, H. B. and Farhad, M., “Antitussive effect of Portulaca oleracea L. in guinea pigs,” Iranian Journal of Pharmaceutical Research, 3. 187-190. 2004.
[23]	Dkhil, M. A., Abdel Moniem, A. E., Al-Quraishy, S. and Saleh, R. A., “Antioxidant effect of purslane (Portulaca oleracea) and its mechanism of action,” Journal of Medicinal Plants Research, 5. 1563-1589. 2011.
[24]	Rashed, A. N., Afifi, F. U. and Disi, A. M., “Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculus JVI-1,” Journal of Ethnopharmacology, 88. 131-136. 2003.
[25]	Zhang, Z.F., Lv, G.Y., He, W.Q., Shi, L.G., Pan, H.G. and Fan, L. F., “Effects of extraction methods on the antioxidant activities of polysaccharides obtained from Flammulina velutipes, ” Carbohydrate Polymers, 98 (2013). 1524-1531. 2013.
[26]	Chen, A.M., Ning, W., Yu, R.H., Li, B.C. and Li, F., “The research of polysaccharide extraction method from Purslane,” Am ino Acid s& B iotic R esources, 28 (1). 10-11. 2006.
[27]	Liang,Y.,.”Extraction of purslane polysaccharides by microwave assisted process optimization,” Guizhou agricultural science, 40.148-150. 2012.
[28]	Tao, Y. Z. and Xu, W. L., “Microwave-assisted solubilization and solution properties of hyperbranched polysaccharide,” Carbohydrate Research, 343.3071-3078. 2008.
[29]	Marshall, L. F., Chau, H. K., Cooke, P. H., Yadav, M. P. and Hotchkiss, A. T., “Physico-chemical characterization of alkaline soluble polysaccharides from sugar beet pulp,” Food Hydrocolloids, 23. 1554-1562. 2009.
[30]	Yu, L., “Ultrasonic aided method of extraction of purslane polysaccharides,” The north garden, 5, 36-38. 2011
[31]	Zhang, Y.M., Li,B.C., Zhu, L.P., Dai, W.F. and Fan, J.H., “Polysaccharide chemistry and biological research progress,” Journal of kunming university of science and technology, 28,140-148. 2003.
[32]	Tian, G.H., Liu, C.F. and Lai, P.H., “Purslane experimental study on the ultrasonic extraction and activity of polysaccharides,” Food research and development, 128. 7-10. 2007.