Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: https://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
Open Access
Journal Browser
Go
Journal of Food and Nutrition Research. 2025, 13(3), 114-126
DOI: 10.12691/jfnr-13-3-1
Open AccessArticle

Sustainable Local Gluten-free Flours: Nutritional, Physical, and Functional Characterization

Wafa Allouch Tounsi1, 2, , Dorra Sfayhi Terras1, Nessrine Haj Yahia1, Youkebed Zarroug1, Leila Doggui3, Mariem Bouhadida1, Ali Ouji1, Khalil Kamessi1, Mohamed Kharrat1 and Hajer Debbabi2

1Field Crop Laboratory (LR16INRAT02), National Institute of Agronomic Research of Tunisia, University of Carthage, Rue Hedi Karray, Ariana 2049, Tunisia

2Food Science and Technology Department (UR17AGR01), National Agronomic Institute of Tunisia, Carthage University, 43, Charles Nicolle Avenue – 1082 Tunis- Mahrajène- Tunisia

3Competitiveness pole of Bizerte, Union du Grand Maghreb Arab boulevard - 7080 Menzel Jemil, Bizerte, Tunisia

Pub. Date: March 16, 2025

Cite this paper:
Wafa Allouch Tounsi, Dorra Sfayhi Terras, Nessrine Haj Yahia, Youkebed Zarroug, Leila Doggui, Mariem Bouhadida, Ali Ouji, Khalil Kamessi, Mohamed Kharrat and Hajer Debbabi. Sustainable Local Gluten-free Flours: Nutritional, Physical, and Functional Characterization. Journal of Food and Nutrition Research. 2025; 13(3):114-126. doi: 10.12691/jfnr-13-3-1

Abstract

Most of gluten-free (GF) bakery products available on the market are made with a restricted number of grains. Flours and starches from rice and maize are mainly used; for this reason, people affected by celiac disease frequently suffer from nutritional deficiencies. The use of a wider range of GF flours, rich in nutrients and phytochemicals, may improve the nutritional quality of GF products. In this work, an investigation of the physicochemical, mineral, and functional profiles in 16 GF flours belonging to different local varieties of legumes (chickpea, fava bean, and lentil), cereals (rice and maize) and pseudo-cereals (white quinoa, red quinoa and chia) was achieved. Significant differences could be observed across samples, legume flours presented very interesting nutritional characteristics: proteins (22 % - 28.71%), mineral composition especially in iron (52.05ppm to 152.45ppm), Zinc (36.96% - 51.28%) and Potassium (0.83% -1.13%); moreover, pulses were characterized by their high antioxidant activity (88.63%). Besides the important functional properties, such as the emulsifying activity and stability as well as the foaming capacity and foam stability, were noticed especially for lentil and fava bean flours. Finally, such interesting properties contribute to the selection of flours for healthier GF bakery products.

Keywords:
flours gluten-free functional properties physicochemical characterization

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Figures

Figure of 1

References:

[1]  Peter, H., M. Green, and M. Christophe, Celiac disease. N Engl J Med, 2007. 357: p. 1731-43.
 
[2]  Shan, L., et al., Structural basis for gluten intolerance in celiac sprue. Science, 2002. 297(5590): p. 2275-2279.
 
[3]  Green, P.H. and B. Jabri, Coeliac disease. The Lancet, 2003. 362(9381): p. 383-391.
 
[4]  Fasano, A. and C. Catassi, Celiac disease. New England Journal of Medicine, 2012. 367(25): p. 2419-2426.
 
[5]  Leonard, M.M., et al., Celiac disease and nonceliac gluten sensitivity: a review.Jama, 2017. 318(7): p. 647-656.
 
[6]  Alimentarius, C., Norme pour les aliments diététiques ou de régime destinés aux personnes souffrant d’une intolérance au gluten. CODEX STAN 118-1979. Amendement : 1983 et 2015., Révision : 2008, Adoptée en 1979.
 
[7]  Rubio-Tapia, A., et al., American College of Gastroenterology clinical guideline: diagnosis and management of celiac disease.The American journal of gastroenterology, 2013. 108(5): p. 656.
 
[8]  Rosell, C.M., et al., Cereals for developing gluten-free products and analytical tools for gluten detection. Journal of cereal science, 2014. 59(3): p. 354-364.
 
[9]  Gorinstein, S., et al., Use of scanning electron microscopy to indicate the similarities and differences in pseudocereal and cereal proteins. International journal of food science & technology, 2004. 39(2): p. 183-189.
 
[10]  Martínez-Villaluenga, C., E. Peñas, and B. Hernández-Ledesma, Pseudocereal grains: Nutritional value, health benefits and current applications for the development of gluten-free foods. Food and Chemical Toxicology, 2020. 137: p. 111178.
 
[11]  Aguilar, N., et al., Chickpea and tiger nut flours as alternatives to emulsifier and shortening in gluten-free bread. LWT-Food science and Technology, 2015. 62(1): p. 225-232.
 
[12]  Flores‐Silva, P.C., et al., Glutenfree spaghetti made with chickpea, unripe plantain and maize flours: functional and chemical properties and starch digestibility. International Journal of Food Science & Technology, 2014. 49(9): p. 1985-1991.
 
[13]  Gularte, M.A., M. Gómez, and C.M. Rosell, Impact of legume flours on quality and in vitro digestibility of starch and protein from gluten-free cakes. Food and Bioprocess Technology, 2012. 5(8): p. 3142-3150.
 
[14]  Herranz, B., et al., Characterisation of chickpea flourbased glutenfree batters and muffins with added biopolymers: rheological, physical and sensory properties. International Journal of Food Science & Technology, 2016. 51(5): p. 1087-1098.
 
[15]  Meng, X., et al., Effects of extrusion conditions on system parameters and physical properties of a chickpea flour-based snack. Food Research International, 2010. 43(2): p. 650-658.
 
[16]  Boye, J., et al., Comparison of the functional properties of pea, chickpea and lentil protein concentrates processed using ultrafiltration and isoelectric precipitation techniques. Food Research International, 2010. 43(2): p. 537-546.
 
[17]  Karaca, A.C., N. Low, and M. Nickerson, Emulsifying properties of chickpea, faba bean, lentil and pea proteins produced by isoelectric precipitation and salt extraction. Food Research International, 2011. 44(9): p. 2742-2750.
 
[18]  Aydemir, L.Y. and A. Yemenicioğlu, Potential of Turkish Kabuli type chickpea and green and red lentil cultivars as source of soy and animal origin functional protein alternatives. LWT-Food Science and Technology, 2013. 50(2): p. 686-694.
 
[19]  Khalil, A. and E. Mansour, The effect of cooking, autoclaving and germination on the nutritional quality of faba beans. Food Chemistry, 1995. 54(2): p. 177-182.
 
[20]  Perez-Maldonado, R., P. Mannion, and D. Farrell, Optimum inclusion of field peas, faba beans, chick peas and sweet lupins in poultry diets. I. Chemical composition and layer experiments. British poultry science, 1999. 40(5): p. 667-673.
 
[21]  Derbyshire, E. and J. Delange, The nutritional value of whole pulses and pulse fractions, in Pulse Foods. 2021, Elsevier. p. 9-29.
 
[22]  Gobbetti, M., et al., Novel insights on the functional/nutritional features of the sourdough fermentation. International journal of food microbiology, 2019. 302: p. 103-113.
 
[23]  Takeoka, G.R., et al., Delphinidin 3-O-(2-O-β-D-glucopyranosyl-α-L-arabinopyranoside): A novel anthocyanin identified in beluga black lentils. Journal of agricultural and food chemistry, 2005. 53(12): p. 4932-4937.
 
[24]  Siddique, K.H., et al., Innovations in agronomy for food legumes. A review. Agronomy for sustainable development, 2012. 32: p. 45-64.
 
[25]  Ramirez-Cabral, N.Y.Z., L. Kumar, and S. Taylor, Crop niche modeling projects major shifts in common bean growing areas. Agricultural and Forest Meteorology, 2016. 218: p. 102-113.
 
[26]  Peltonen-Sainio, P., et al., Climate change and prolongation of growing season: changes in regional potential for field crop production in Finland. 2009.
 
[27]  Yadav, S.S., et al., Climate change and management of cool season grain legume crops. 2010: Springer.
 
[28]  Kim, B.F., et al., Country-specific dietary shifts to mitigate climate and water crises. Global environmental change, 2020. 62: p. 101926.
 
[29]  Kaur, M., N. Singh, and N.S. Sodhi, Physicochemical, cooking, textural and roasting characteristics of chickpea (Cicer arietinum L.) cultivars. Journal of Food Engineering, 2005. 69(4): p. 511-517.
 
[30]  [AOAC] Assn. of Official Analytical Chemists. 2000. Coffee and tea. In: Official methods of analysis. 17th ed. Gaithersburg, Md.: AOAC., 2000.
 
[31]  LST EN 15510. Lithuanian standard, 2017. Animal feeding stuffs: methods of sampling and analysis -Determination of calcium, sodium, phosphorus, magnesium, potassium, iron, zinc, copper, manganese, cobalt, molybdenum and lead by ICP-AES. Vilnius: Lithuanian Standards Board., 2017.
 
[32]  Dewanto, V., et al., Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. Journal of agricultural and food chemistry, 2002. 50(10): p. 3010-3014.
 
[33]  Sun, B., J.M. Ricardo-da-Silva, and I. Spranger, Critical factors of vanillin assay for catechins and proanthocyanidins. Journal of agricultural and food chemistry, 1998. 46(10): p. 4267-4274.
 
[34]  Kedare, S.B. and R. Singh, Genesis and development of DPPH method of antioxidant assay. Journal of food science and technology, 2011. 48(4): p. 412-422.
 
[35]  Marteau, C., et al., Dual role of phenols as fragrances and antioxidants: mechanism, kinetics and drastic solvent effect. Flavour and Fragrance Journal, 2013. 28(1): p. 30-38.
 
[36]  Kaur, M. and K.S. Sandhu, Functional, thermal and pasting characteristics of flours from different lentil (Lens culinaris) cultivars. Journal of food science and technology, 2010. 47(3): p. 273-278.
 
[37]  Anderson, R., et al., Griffin (1969) Gelatinization of corn grits by roll-and extrusion-cooking. Cereal Sci. Today, 1969. 14(4): p. 11.
 
[38]  Sosulski, F., The centrifuge method for determining flour absorption in hard red spring wheats. Cereal Chem., 1962. 39: p. 344-350.
 
[39]  Lin, M.J.-Y., E. Humbert, and F. Sosulski, Certain functional properties of sunflower meal products. Journal of Food Science, 1974. 39(2): p. 368-370.
 
[40]  Sathe, S., S. Deshpande, and D. Salunkhe, Functional properties of lupin seed (Lupinus mutabilis) proteins and protein concentrates. Journal of food science, 1982. 47(2): p. 491-497.
 
[41]  Naczk, M., L. Diosady, and L. Rubin, Functional properties of canola meals produced by a twophase solvent extraction system. Journal of Food Science, 1985. 50(6): p. 1685-1688.
 
[42]  Kaushal, P., V. Kumar, and H.K. Sharma, Comparative study of physicochemical, functional, antinutritional and pasting properties of taro (Colocasia esculenta), rice (Oryza sativa) flour, pigeonpea (Cajanus cajan) flour and their blends. LWT - Food Science and Technology, 2012. 48(1): p. 59-68.
 
[43]  Millar, K.A., et al., Proximate composition and anti-nutritional factors of fava-bean (Vicia faba), green-pea and yellow-pea (Pisum sativum) flour. Journal of Food Composition and Analysis, 2019. 82.
 
[44]  Fujiwara, N., C. Hall, and A.L. Jenkins, Development of low glycemic index (GI) foods by incorporating pulse ingredients into cerealbased products: Use of in vitro screening and in vivo methodologies. Cereal Chemistry, 2017. 94(1): p. 110-116.
 
[45]  Rocchetti, G., et al., Gluten-free flours from cereals, pseudocereals and legumes: Phenolic fingerprints and in vitro antioxidant properties. Food Chemistry, 2019. 271: p. 157-164.
 
[46]  Masey O’Neill, H.V., et al., Standardised ileal digestibility of crude protein and amino acids of UK-grown peas and faba beans by broilers. Animal Feed Science and Technology, 2012. 175(3-4): p. 158-167.
 
[47]  Ghribi, A.M., et al., Nutritional and compositional study of Desi and Kabuli chickpea (Cicer arietinum L.) flours from Tunisian cultivars. Advances in Food Technology and Nutrition Sciences Open Journal, 2015. 1(2): p. 38-47.
 
[48]  Millar, K.A., et al., Effect of pulse flours on the physiochemical characteristics and sensory acceptance of baked crackers. International Journal of Food Science & Technology, 2017. 52(5): p. 1155-1163.
 
[49]  Tazrart, K., et al., Nutrient composition and in vitro digestibility of fresh pasta enriched with Vicia faba. Journal of Food Composition and Analysis, 2016. 47: p. 8-15.
 
[50]  Kaushal, P., V. Kumar, and H. Sharma, Comparative study of physicochemical, functional, antinutritional and pasting properties of taro (Colocasia esculenta), rice (Oryza sativa) flour, pigeonpea (Cajanus cajan) flour and their blends. LWT-Food Science and Technology, 2012. 48(1): p. 59-68.
 
[51]  Daglia, M., Polyphenols as antimicrobial agents. Current opinion in biotechnology, 2012. 23(2): p. 174-181.
 
[52]  Grosso, G., Effects of polyphenol-rich foods on human health. 2018, MDPI. p. 1089.
 
[53]  Di Cairano, M., et al., Functional properties and predicted glycemic index of gluten free cereal, pseudocereal and legume flours. Lwt, 2020. 133.
 
[54]  Johnson, J.B., et al., Phenolic profiles of ten Australian faba bean varieties. Molecules, 2021. 26(15): p. 4642.
 
[55]  Journi, M., et al., Quantitative determination and identification of phenolic compounds of three Tunisian legumes: Vicia faba, Lens culinaris and Phaseolus vulgaris. Advances in Chemistry and Biochemistry Sciences, 2015. 2(3): p. 01-12.
 
[56]  Xu, B.J., S.H. Yuan, and S.K. Chang, Comparative analyses of phenolic composition, antioxidant capacity, and color of cool season legumes and other selected food legumes. J Food Sci, 2007. 72(2): p. S167-77.
 
[57]  Sharma, K., et al., Health effects, sources, utilization and safety of tannins: A critical review. Toxin Reviews, 2021. 40(4): p. 432-444.
 
[58]  Hassanpour, S., N. MaheriSis, and B. Eshratkhah, Plants and secondary metabolites (Tannins): A Review. 2011.
 
[59]  Schmelter, L., H. Rohm, and S. Struck, Gluten-free bakery products: Cookies made from different Vicia faba bean varieties. Future Foods, 2021. 4: p. 100038.
 
[60]  Lizarazo, C.I., et al., Nutritive quality and protein production from grain legumes in a boreal climate. Journal of the Science of Food and Agriculture, 2015. 95(10): p. 2053-2064.
 
[61]  Mayachiew, P. and S. Devahastin, Antimicrobial and antioxidant activities of Indian gooseberry and galangal extracts. LWT-Food Science and Technology, 2008. 41(7): p. 1153-1159.
 
[62]  Marathe, S.A., et al., Comparative study on antioxidant activity of different varieties of commonly consumed legumes in India. Food and Chemical Toxicology, 2011. 49(9): p. 2005-2012.
 
[63]  Zhao, Y., et al., In vitro antioxidant activity of extracts from common legumes. Food chemistry, 2014. 152: p. 462-466.
 
[64]  Morales, D., M. Miguel, and M. Garcés-Rimón, Pseudocereals: a novel source of biologically active peptides. Critical reviews in food science and nutrition, 2021. 61(9): p. 1537-1544.
 
[65]  Kwon, S.-J., et al., Phytochemical compounds and antioxidant activity in the grain of selected faba bean (Vicia faba) genotypes. Plant breeding and biotechnology, 2018. 6(1): p. 65-73.
 
[66]  Campos-Vega, R., G. Loarca-Piña, and B.D. Oomah, Minor components of pulses and their potential impact on human health. Food research international, 2010. 43(2): p. 461-482.
 
[67]  Babu, R.P., K. O'connor, and R. Seeram, Current progress on bio-based polymers and their future trends. Progress in biomaterials, 2013. 2: p. 1-16.
 
[68]  Wang, Y., et al. CDnet 2014: An expanded change detection benchmark dataset. in Proceedings of the IEEE conference on computer vision and pattern recognition workshops. 2014.
 
[69]  Thongram, S., et al., Physicochemical and organoleptic properties of cookies incorporated with legume flours. Cogent Food & Agriculture, 2016. 2(1): p. 1172389.
 
[70]  Drakula, S., et al., Alteration of phenolics and antioxidant capacity of gluten-free bread by yellow pea flour addition and sourdough fermentation. Food Bioscience, 2021. 44: p. 101424.
 
[71]  Barden, L. and E.A. Decker, Lipid oxidation in low-moisture food: A review. Critical reviews in food science and nutrition, 2016. 56(15): p. 2467-2482.
 
[72]  Commission, E., EU Register on nutrition and health claims. 2016.
 
[73]  Ray, H., et al., Mineral micronutrient content of cultivars of field pea, chickpea, common bean, and lentil grown in Saskatchewan, Canada. Crop Science, 2014. 54(4): p. 1698-1708.
 
[74]  De Angelis, D., et al., Antinutritional factors, mineral composition and functional properties of dry fractionated flours as influenced by the type of pulse. Heliyon, 2021. 7(2): p. e06177.
 
[75]  Authority, E.F.S., Dietary reference values for nutrients summary report. 2017, Wiley Online Library.
 
[76]  Grela, E.R., et al., Content of minerals and fatty acids and their correlation with phytochemical compounds and antioxidant activity of leguminous seeds. Biological Trace Element Research, 2017. 180: p. 338-348.
 
[77]  Gordon, B., Manganese nutrition of glyphosate-resistant and conventional soybeans. Better Crops, 2007. 91(4): p. 12-13.
 
[78]  Culetu, A., et al., Nutritional and functional properties of gluten-free flours. Applied Sciences, 2021. 11(14): p. 6283.
 
[79]  Joshi, A.U., C. Liu, and S.K. Sathe, Functional properties of select seed flours. LWT-Food Science and Technology, 2015. 60(1): p. 325-331.
 
[80]  Di Cairano, M., et al., Functional properties and predicted glycemic index of gluten free cereal, pseudocereal and legume flours. Lwt, 2020. 133: p. 109860.
 
[81]  Kaur, M. and N. Singh, Studies on functional, thermal and pasting properties of flours from different chickpea (Cicer arietinum L.) cultivars. Food chemistry, 2005. 91(3): p. 403-411.
 
[82]  !!! INVALID CITATION !!! [9].
 
[83]  Du, S.-k., et al., Physicochemical and functional properties of whole legume flour. LWT - Food Science and Technology, 2014. 55(1): p. 308-313.
 
[84]  Bouasla, A., A. Wójtowicz, and M.N. Zidoune, Gluten-free precooked rice pasta enriched with legumes flours: Physical properties, texture, sensory attributes and microstructure. Lwt, 2017. 75: p. 569-577.
 
[85]  Jamin, F.F. and R.A. Flores, Effect of additional separation and grinding on the chemical and physical properties of selected corn drymilled streams. Cereal Chemistry, 1998. 75(1): p. 166-170.
 
[86]  Aboubakar, N.Y., J. Scher, and C. Mbofung, Physicochemical, thermal properties and microstructure of six varieties of taro (Colocasia esculenta L. Schott) flours and starches. Journal of Food Engineering, 2008. 86(2): p. 294-305.
 
[87]  Kaur, M., et al., Gluten free biscuits prepared from buckwheat flour by incorporation of various gums: Physicochemical and sensory properties. LWT-Food Science and Technology, 2015. 62(1): p. 628-632.
 
[88]  Du, S.-k., et al., Physicochemical and functional properties of whole legume flour. LWT-Food Science and Technology, 2014. 55(1): p. 308-313.
 
[89]  Patil, S.P. and S.S. Arya, Nutritional, functional, phytochemical and structural characterization of gluten-free flours. Journal of Food Measurement and Characterization, 2017. 11: p. 1284-1294.
 
[90]  Mortuza, M.G., M.A. Hannan, and J.T. Tzen, Chemical composition and functional properties of Vicia faba L. from Bangladesh. Bangladesh Journal of Botany, 2009. 38(1): p. 93-97.
 
[91]  Chandra, S., S. Singh, and D. Kumari, Evaluation of functional properties of composite flours and sensorial attributes of composite flour biscuits. Journal of food science and technology, 2015. 52: p. 3681-3688.