Probiotic Lactiplantibacillus Plantarum JS5 Lowers Cholesterol by Modulating the Gut Microbiota in the High-cholesterol Diet-fed Mice

Yazhou Mao¹, Rong Ren¹, Shuanghong Song², Yuanyuan Hui¹, Linqiang Li¹, Shan Wu³, Aiqing Zhao¹ and Bini Wang^1,

¹College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an, Shaanxi, 710119, China

²Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry of the Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi’an, Shaanxi, 710119, China

³Research and Development center, Xi'an Yinqiao Dairy (Group) Co., Ltd, Xi'an, 710075 China

Cite this paper:
Yazhou Mao, Rong Ren, Shuanghong Song, Yuanyuan Hui, Linqiang Li, Shan Wu, Aiqing Zhao and Bini Wang. Probiotic Lactiplantibacillus Plantarum JS5 Lowers Cholesterol by Modulating the Gut Microbiota in the High-cholesterol Diet-fed Mice. Journal of Food and Nutrition Research. 2023; 11(6):441-453. doi: 10.12691/jfnr-11-6-6

Abstract

Hypercholesterolemia is the major cause of cardiovascular ailments worldwide. Jiang-shui contains plenty of lactic acid bacteria (LAB), although nothing is known about its in vitro probiotic characteristics and cholesterol-lowering effect. Thirty-eight strains were isolated, identified, and classified into Lactiplantibacillus plantarum, Limosilactobacillus fermentum, and Lactobacillus delbrueckii subsp. In this study, the top five strains with the highest cholesterol-lowering rates were selected, and strain L. plantarum JS5 demonstrated satisfactory in vitro probiotic characteristics. L. plantarum JS5 ingestion increased serum HDL-C levels and suppressed the increase in body weight and serum cholesterol induced by a high-cholesterol diet. After L. plantarum JS5 treatment, the relative abundance of the genera Eubacterium_coprostanoligenes_group, Faecalibaculum, and Blautia increased significantly, whereas the genus DesulfovibrIo decreased significantly, improving the intestinal flora composition. In addition, functional analysis revealed that L. plantarum JS5 consumption influenced intestinal flora metabolism in high-cholesterol diet-fed mice in defense, signal transduction, and cellular activity. Hence, L. plantarum JS5 isolated from Jiang-shui might be ideal for preventing and treating hypercholesterolemia.

Keywords:
Lactiplantibacillus plantarum cholesterol-lowering probiotics intestinal flora High-cholesterol Diet

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