Five Natural Active Ingredients Achieve Anti-fatigue Function by Synergistic Antioxidation and Regulating the Structure of Intestinal Flora

Jie Zhou¹, Shuyi Chang¹, Qianxi Zhang¹, Lianxiao Lu¹, Long Li¹ and Zhicheng Li^1,

¹College of Food Science and Engineering, Northwest A&F University, 712100 Yangling, Shaanxi, China

Cite this paper:
Jie Zhou, Shuyi Chang, Qianxi Zhang, Lianxiao Lu, Long Li and Zhicheng Li. Five Natural Active Ingredients Achieve Anti-fatigue Function by Synergistic Antioxidation and Regulating the Structure of Intestinal Flora. Journal of Food and Nutrition Research. 2022; 10(10):655-663. doi: 10.12691/jfnr-10-10-3

Abstract

In this study, the synergistic anti-fatigue effects of five natural active ingredients (anthocyanin, tea polyphenol, Lycium barbarum polysaccharide, ginsenoside, salidroside) and their effects on intestinal flora in mice were investigated. Synergistic antioxidant in vitro showed that when ginsenoside: Lycium barbarum polysaccharide: salidroside = 2:1:6 (4 mg/mL), the maximum experimental scavenging capacity (ESC) of the system was 124.73±5.32 μmol Trolox/L, the synergistic effect (SE) was 2.75. Natural active ingredients of different concentrations and formulations were orally administered to mice. The results showed that compared with control group, the group H (Ginsenoside/Lycium barbarum polysaccharide/Salidroside (2/1/6) 200 mg kg^-1 d^-1) can significantly increase the exhausting swimming time, decreasing the content of blood lactic acid (BLA), serum urea nitrogen (BUN), and malondialdehyde (MDA), which were accompanied by a corresponding increase in liver superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), liver glycogen content (LG) (p < 0.05). The relative abundances of Verrucomicrobiota and Rokubacteria in group H were significantly decreased (p < 0.05), and the relative abundances of Acidobacteria, Parasutterella and Deferribacteres were significantly increased (p < 0.05). These data suggest that ginsenoside, Lycium barbarum polysaccharide, and salidroside achieve synergistic anti-fatigue effects by regulating intestinal flora and oxidative stress.

Keywords:
natural active ingredients antioxidant synergistic effect anti-fatigue intestinal flora

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