Orostachys japonicus Suppresses IFN-γ and TNF-α Induced Proinflammatory Chemokines in Atopic Dermatitis Relating HaCaT Cells via Down-regulation of Expression and Translocation of NF-κB

Yoo Jung Jeon¹, Sung Hyun Kim² and Dong Seok Lee^{1, 3,}

¹Institute of Digital Anti-aging Healthcare, Graduate School, Inje University, Gimhae 50834, Republic of Korea

²Department of Smart Foods and Drugs, Graduate School, Inje University, Gimhae 50834, Republic of Korea

³Department of Smart Foods and Drugs, Graduate School, Inje University, Gimhae 50834, Republic of Korea;Department of Biomedical Laboratory Science, Inje University, 197 Inje-ro, Gimhae 50834, Gyeongsangnam-do, Republic of Korea

Cite this paper:
Yoo Jung Jeon, Sung Hyun Kim and Dong Seok Lee. Orostachys japonicus Suppresses IFN-γ and TNF-α Induced Proinflammatory Chemokines in Atopic Dermatitis Relating HaCaT Cells via Down-regulation of Expression and Translocation of NF-κB. Journal of Food and Nutrition Research. 2022; 10(6):415-424. doi: 10.12691/jfnr-10-6-5

Abstract

To investigate the anti-atopic dermatitis (AD) activity of dichloromethane (DCM) fraction of ethanol extracts from Orostachys japonicus (OJ) in AD relating human keratinocytes (HaCaT cells) through down-regulation of a key transcription factor NF-κB involved in skin inflammatory responses. The DCM fraction was obtained from OJ ethanol extracts and used for this study. For anti-AD activity, HaCaT cells that have been induced with IFN-γ and TNF-α at the same concentration to produce proinflammatory chemokines were used. As analysis methods, WST assay, chemokines measurement, antibody array, nuclear/cytoplasmic protein separation, and western blotting were used. WST assay showed no toxicity to cell viability. The production levels of chemokines such as MDC, RANTES, IL-8, and TARC decreased in a concentration-dependent manner. Immunoassay through the antibody array showed that the DCM fraction down-regulated the NF-κB (p65) expression. In addition, as a result of western blotting, it was revealed that the translocation of NF-κB (p65) from the cytoplasm into the nucleus decreased. The DCM fraction from OJ ethanol extracts showed significant anti-AD activity through suppression of proinflammatory chemokines causing AD in HaCaT cells via down-regulation of expression and translocation of NF-κB (p65) into the nucleus.

Keywords:
Orostachys japonicas atopic dermatitis keratinocyte chemokine HaCaT NF-κB (p65)

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