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Choi, S.Y., Chung, M.J., Seo, W.D., Shin, J.H., Shon, M.Y. and Sung, N.J., “Inhibitory Effects of Orostachys japonicus Extracts on the Formation of N-Nitrosodimethylamine,” J Agric Food Chem, 54. 6075-6078. Jul.2006.

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Article

Orostachys japonicus Suppresses IFN-γ and TNF-α Induced Proinflammatory Chemokines in Atopic Dermatitis Relating HaCaT Cells via Down-regulation of Expression and Translocation of NF-κB

1Institute of Digital Anti-aging Healthcare, Graduate School, Inje University, Gimhae 50834, Republic of Korea

2Department of Smart Foods and Drugs, Graduate School, Inje University, Gimhae 50834, Republic of Korea

3Department of Smart Foods and Drugs, Graduate School, Inje University, Gimhae 50834, Republic of Korea;Department of Biomedical Laboratory Science, Inje University, 197 Inje-ro, Gimhae 50834, Gyeongsangnam-do, Republic of Korea


Journal of Food and Nutrition Research. 2022, Vol. 10 No. 6, 415-424
DOI: 10.12691/jfnr-10-6-5
Copyright © 2022 Science and Education Publishing

Cite this paper:
Yoo Jung Jeon, Sung Hyun Kim, Dong Seok Lee. Orostachys japonicus Suppresses IFN-γ and TNF-α Induced Proinflammatory Chemokines in Atopic Dermatitis Relating HaCaT Cells via Down-regulation of Expression and Translocation of NF-κB. Journal of Food and Nutrition Research. 2022; 10(6):415-424. doi: 10.12691/jfnr-10-6-5.

Correspondence to: Dong  Seok Lee, Institute of Digital Anti-aging Healthcare, Graduate School, Inje University, Gimhae 50834, Republic of Korea. Email: mbdslee@inje.ac.kr

Abstract

To investigate the anti-atopic dermatitis (AD) activity of dichloromethane (DCM) fraction of ethanol extracts from Orostachys japonicus (OJ) in AD relating human keratinocytes (HaCaT cells) through down-regulation of a key transcription factor NF-κB involved in skin inflammatory responses. The DCM fraction was obtained from OJ ethanol extracts and used for this study. For anti-AD activity, HaCaT cells that have been induced with IFN-γ and TNF-α at the same concentration to produce proinflammatory chemokines were used. As analysis methods, WST assay, chemokines measurement, antibody array, nuclear/cytoplasmic protein separation, and western blotting were used. WST assay showed no toxicity to cell viability. The production levels of chemokines such as MDC, RANTES, IL-8, and TARC decreased in a concentration-dependent manner. Immunoassay through the antibody array showed that the DCM fraction down-regulated the NF-κB (p65) expression. In addition, as a result of western blotting, it was revealed that the translocation of NF-κB (p65) from the cytoplasm into the nucleus decreased. The DCM fraction from OJ ethanol extracts showed significant anti-AD activity through suppression of proinflammatory chemokines causing AD in HaCaT cells via down-regulation of expression and translocation of NF-κB (p65) into the nucleus.

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