Beneficial Effects of Macaroni Made with Resistant Starch Type 4 from Unripe Banana and Turmeric Extract on Blood Clinical Chemistry and Gut Microbiota of Healthy Rats

Wongsakan Chuathong¹, Wathinee Phomsakha Na Sakonnakhon², Atitayaporn Lilakhon¹, Natthaporn Devahastin Na Ayudhaya¹, Worapong Khaodee¹, Singkome Tima^{3, 4}, Nutjeera Intasai^{3, 4}, Rujirek Chaiwongsa^{4, 5}, Sawitree Chiampanichayakul^{3, 4}, Khanittha Punturee^{1, 4} and Ratchada Cressey^{1, 4,}

¹Division of Clinical Chemistry, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Thailand

²Laboratory Animal Center, Chiang Mai University, Thailand

³Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Thailand

⁴Cancer Research Unit of Associated Medical Sciences (AMS-CRU), Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand

⁵Division of Blood Transfusion Science, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Thailand

Cite this paper:
Wongsakan Chuathong, Wathinee Phomsakha Na Sakonnakhon, Atitayaporn Lilakhon, Natthaporn Devahastin Na Ayudhaya, Worapong Khaodee, Singkome Tima, Nutjeera Intasai, Rujirek Chaiwongsa, Sawitree Chiampanichayakul, Khanittha Punturee and Ratchada Cressey. Beneficial Effects of Macaroni Made with Resistant Starch Type 4 from Unripe Banana and Turmeric Extract on Blood Clinical Chemistry and Gut Microbiota of Healthy Rats. Journal of Food and Nutrition Research. 2021; 9(7):329-341. doi: 10.12691/jfnr-9-7-2

Abstract

Unripe banana is rich with resistant starch (RS) and this non-processed food confers many health benefits. However, unripe banana is not consumed directly and when cooked its native RS is rendered digestible. We have developed macaroni from chemically modified unripe banana flour (RS4), which maintains their content of resistant starch after being cooked, with and without the supplementation of turmeric extract. We hypothesized that consuming our banana RS4 macaroni would confer beneficial effects on metabolic profiles. Healthy Wistar rats were fed for 6 weeks with cooked banana RS4 macaroni, with and without the turmeric extract supplementation, and compared to rats fed with standard wheat macaroni. No significant physiological differences between groups were observed except rats that consumed banana RS4 macaroni had significantly smaller stomachs (p<0.05). Rats receiving banana RS4 macaroni had significantly lower triglyceride levels (p<0.05) and a trend for lower fasting blood glucose and increased expression of insulin-like growth factor-2 in theirs livers. Although it was not statistically significant, turmeric supplementation showed a trend of reducing serum total cholesterol and LDL-cholesterol as well as liver expression levels of genes involved in cholesterol metabolism, HMG CoA reductase (HMGR) and LDL-receptor related protein-1 (LRP-1). Banana RS4 macaroni did not significantly change fecal microbiota, nevertheless, addition of turmeric extract significantly increased alpha diversity and the relative abundance of Lachnospiraceae Erysipelotrichaceae and Clostridiaceae. In conclusion, the consumption of banana RS4 macaroni by healthy rats receiving a regular diet improved their blood chemistry profile associated with metabolic syndrome, and the addition of turmeric extract can significantly alter fecal microbiota.

Keywords:
metabolic syndrome functional foods Resistant Starch type 4 (RS4) unripe banana flour gut microbiota turmeric

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