Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2021, 9(2), 61-67
DOI: 10.12691/jfnr-9-2-1
Open AccessArticle

Inhibitory Activity of Fruits Extracts of Antidesma bunius on the Proliferation and Migration of MDA-MB-231 Breast Cancer Cells

Ma Funing1, Tariq Masood1, 2, , Huang Dongmei1, Wu Bin1, Ge Yu1, Chen Di1, Gutierrez-Pajares Jorge L1, Nasiruddin3 and Song Shun1,

1Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences / Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Hainan 571101, China

2Department of Agricultural Chemistry, The University of Agriculture, Peshawar, Pakistan

3Direcotrate General Agricultural Research, Khyber Pakhtunkhwa, Peshawar, Pakistan

Pub. Date: January 31, 2021

Cite this paper:
Ma Funing, Tariq Masood, Huang Dongmei, Wu Bin, Ge Yu, Chen Di, Gutierrez-Pajares Jorge L, Nasiruddin and Song Shun. Inhibitory Activity of Fruits Extracts of Antidesma bunius on the Proliferation and Migration of MDA-MB-231 Breast Cancer Cells. Journal of Food and Nutrition Research. 2021; 9(2):61-67. doi: 10.12691/jfnr-9-2-1


In this research, we investigated the inhibitory effect of the ethanolic (EtOH) extract of bignay (Antidesma bunius) fruit on the viability of breast cancer cells (MDA-MB-231) by CCK-8 assay. The results showed that the half inhibitory concentration (IC50) was 219 μg/mL after the cells were treated with EtOH extract for 72 hours. The EtOH extract was sequentially extracted by petroleum ether (PET), ethyl acetate (EtOAc), and n-butanol (nBuOH). Among them, the EtOAc fraction showed higher anti-proliferative activity than the other fractions. Wound healing experiment showed the EtOAc fraction inhibited the migration of MDA-MB-231 cells and significantly delayed the transition of the G1 to S phase compared to control. UHPLC-MS/MS analysis showed that in addition to citric acid and phenolic acids (3-Hydroxybenzoic acid, aspirin, fertaric acid), EtOH extract of bignay fruit contains active ingredient like coumarin, genistin, amentoflavone, and luteolin 7-galactoside. The anti-proliferation activity of amentoflavone against MDA-MB-231 cells was also confirmed.

breast cancer ethno-medicine flavonoid tropical fruits

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