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Bao, C., Chen, J., Kim, J. T., Qiu, S., Cho, J. S and Lee, H. J. Amentoflavone inhibits tumorsphere formation by regulating the Hedgehog/Gli1 signaling pathway in SUM159 breast cancer stem cells. J Func Foods, 61: 103501. 2019.

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Article

Inhibitory Activity of Fruits Extracts of Antidesma bunius on the Proliferation and Migration of MDA-MB-231 Breast Cancer Cells

1Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences / Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Hainan 571101, China

2Department of Agricultural Chemistry, The University of Agriculture, Peshawar, Pakistan

3Direcotrate General Agricultural Research, Khyber Pakhtunkhwa, Peshawar, Pakistan


Journal of Food and Nutrition Research. 2021, Vol. 9 No. 2, 61-67
DOI: 10.12691/jfnr-9-2-1
Copyright © 2021 Science and Education Publishing

Cite this paper:
Ma Funing, Tariq Masood, Huang Dongmei, Wu Bin, Ge Yu, Chen Di, Gutierrez-Pajares Jorge L, Nasiruddin, Song Shun. Inhibitory Activity of Fruits Extracts of Antidesma bunius on the Proliferation and Migration of MDA-MB-231 Breast Cancer Cells. Journal of Food and Nutrition Research. 2021; 9(2):61-67. doi: 10.12691/jfnr-9-2-1.

Correspondence to: Song  Shun, Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences / Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Hainan 571101, China. Email: sss1984006@163.com, tariqafridi@aup.edu.pk

Abstract

In this research, we investigated the inhibitory effect of the ethanolic (EtOH) extract of bignay (Antidesma bunius) fruit on the viability of breast cancer cells (MDA-MB-231) by CCK-8 assay. The results showed that the half inhibitory concentration (IC50) was 219 μg/mL after the cells were treated with EtOH extract for 72 hours. The EtOH extract was sequentially extracted by petroleum ether (PET), ethyl acetate (EtOAc), and n-butanol (nBuOH). Among them, the EtOAc fraction showed higher anti-proliferative activity than the other fractions. Wound healing experiment showed the EtOAc fraction inhibited the migration of MDA-MB-231 cells and significantly delayed the transition of the G1 to S phase compared to control. UHPLC-MS/MS analysis showed that in addition to citric acid and phenolic acids (3-Hydroxybenzoic acid, aspirin, fertaric acid), EtOH extract of bignay fruit contains active ingredient like coumarin, genistin, amentoflavone, and luteolin 7-galactoside. The anti-proliferation activity of amentoflavone against MDA-MB-231 cells was also confirmed.

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