Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2020, 8(7), 313-319
DOI: 10.12691/jfnr-8-7-2
Open AccessArticle

Chlorogenic Acid Decreases Lipid Accumulation in 3T3-L1 Adipocytes by Modulating the Transcription Factors

Ching-Chih Liu1, Jen-Yin Chen2, 3, Chin-Chen Chu3, Shih-Ying Chen4, Heuy-Ling Chu5 and Pin-Der Duh5,

1Department of Ophthalmology Chi-Mei Medical Center, 710402, Taiwan, R.O.C.

2Department of Senior Citizen Service Management, Chia Nan University of Pharmacy and Science, 71710, Taiwan, R.O.C.

3Department of Anesthesiology, Chi-Mei Medical Center, 710402, Taiwan, R.O.C.

4Department of Health and Nutrition, Chia Nan University of Pharmacy and Science, Tainan, 71710,Taiwan, R.O.C.

5Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, 71710,Taiwan, R.O.C.

Pub. Date: July 29, 2020

Cite this paper:
Ching-Chih Liu, Jen-Yin Chen, Chin-Chen Chu, Shih-Ying Chen, Heuy-Ling Chu and Pin-Der Duh. Chlorogenic Acid Decreases Lipid Accumulation in 3T3-L1 Adipocytes by Modulating the Transcription Factors. Journal of Food and Nutrition Research. 2020; 8(7):313-319. doi: 10.12691/jfnr-8-7-2


The aim of this study is to investigate the effect of chlorogenic acid (CA) on 3T3-L1 adipocytes and its mechanism action. CA at 1-25 μM showed no cytotoxicity to 3T3-L1 pre-adipocytes and 3T3-L1 adipocytes. CA significantly inhibited oil red O-stained material (OROSM) and intracellular triglyceride levels in 3T3-L1 adipocytes in a concentration-dependent manner. In addition, CA down-regulated the glycerol-3–phosphate dehydrogenase (GPDH) and peroxisome proliferator–activated receptor γ (PPARγ) activity. A real-time polymerase chain reaction revealed that CA inhibited the PPARγ, CCAAT/enhancer-binding protein alpha (C/EBPα), sterol regulatory element binding protein-1c (SREBP-1c), and fatty acid synthase (FAS) gene expression, which may in part account for anti-adipogenesis of CA. Thus, CA could act as a potential lipid lowering functional resource.

chlorogenic acid adipocytes anti-adipogenesis lipid accumulation transcription factor

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[1]  WHO, Obesity and overweight, 2020 room/fact-sheets/detail/obesity-and-overweight.
[2]  Tzeng, T.F., Lu, H.J., Liou, S.S., Chang, C.J., Liu, I.M., “Reduction of lipid accumulation in white adipose tissues by Cassia tora (Leguminosae) seed extract is associated with AMPK activation”, Food Chem, 136, 1086-1094, 2013.
[3]  Chyau, C.C., Chu, C.C., Chen, S.Y., Duh, P.D., “The inhibitory effects of djulis (Chenopodium formosanum) and its bioactive compounds on adipogenesis in 3T3-L1 adipocytes”, Molecules, 23(7), 1780, 2018.
[4]  Chen, S.H., Chu, C.C., Lin, Y.C., Duh, P.D., “Djulis (Chenopodium formosanum) and its bioactive compounds for management of hyperlipidemia and hyperglycemia in high-fat diet-fed mice”, J Food Nutr Res, 7, 452-457, 2019.
[5]  Kowalska, K., Olejnik, A., Rychlik, J., Graje, W., “Cranberries (Oxycoccus quadripetalus) inhibit adipogenesisand lipogenesis in 3T3-L1 cells”, Food Chem, 148, 246-252, 2014.
[6]  Xu, J.G., Hu, Q.P., Liu, Y., “Antioxidant and DNA-protective activities of chlorogenic acid isomers”, J Agric. Food Chem, 60, 11625-11630, 2012.
[7]  Limwachiranon, J., Huang, H., Li, L., Lin, X., Zou, L., Liu, J., Zou, Y., Aalim, H., Duan, Z., Luo, Z., “Enhancing stability and bioaccessibility of chlorogenic acid using complexation with amylopectin: A comprehensive evaluation of complex formation, properties, and characteristics”, Food Chem, 311, 125879, 2020.
[8]  Hsu, C.L., Yen G.C., “Effects of flavonoids and phenolic acids on the inhibition of adipogenesis in 3T3-L1 adipocytes”, J Agric Food Chem, 55(21), 8404-8410, 2007.
[9]  Tzeng, T.F., Liu, I.M., “6-gingerol prevents adipogenesis and the accumulation of cytoplasmic lipid droplets in 3T3-L1 cells”, Phytomedicine, 20(6), 481-487, 2013.
[10]  Noh, J.R., Kim, Y.H., Hwang, J.H., Gang, G.T., Yeo, S.H., Kim, K.S., Oh, W.K., Ly, S.Y., Lee, I.K., Lee, C.H.,” Scoparone inhibits adipocyte differentiation through down-regulation of peroxisome proliferators-activated receptor γ in 3T3-L1 preadipocytes”, Food Chem, 141(2),723-730. 2013.
[11]  Zhao, Y., Li, X., Wang, F., Zhao, X., Gao, Y., Zhao, C., He, L., Zuotong Li, Z., Xu, J., “Glycerol-3-phosphate dehydrogenase (GPDH) gene family in Zea mays L.: Identification, subcellular localization, and transcriptional responses to abiotic stresses”, PLOS ONE. 2018.
[12]  Tan, X.C., Chua, K.H., Ram, M.R., Kuppusamy, U.R., “Monoterpenes: Novel insights into their biological effects and roles on glucose uptake and lipid metabolism in 3T3-L1 adipocytes”, Food Chem, 196, 242-250, 2016.
[13]  Chen, Y.Y., Lee, M.H., Hsu, C.C., Wei, C.L., Tsai, Y.C. “Methyl cinnamate inhibits adipocyte differentiation via activation of the CaMKK2–AMPK pathway in 3T3-L1 preadipocytes”, J Agric Food Chem, 60, 4, 955-963, 2012.
[14]  Fujimori, K.; Shibano, M., “Avicularin, a plant flavonoid, suppresses lipid accumulation through repression of C/EBPalpha-activated GLUT4-mediated glucose uptake in 3T3-L1 cells”, J Agric Food Chem, 61, 5139-5147, 2013.
[15]  Kowalska, K., Olejnik, A., Rychlik, J., Grajek, W., “Cranberries (Oxycoccus quadripetalus) inhibit adipogenesis and lipogenesis in 3T3-L1 cells”, Food Chem, 148, 246-252, 2016.
[16]  Ogawa, T., Tabata, H., Katsube, T., Ohta, Y., Shiwaku, K., “Suppressive effect of hot water extract of wasabi (Wasabia japonica Matsum.) leaves on the differentiation of 3T3-L1 preadipocytes”, Food Chem, 118, 239-244, 2010.
[17]  Domínguez-Avila, J.A., Alvarez-Parrilla, E., López-Díaz, J.A., Maldonado-Mendoza, I.E., Gómez-García, M.D.C., de la Rosa, L.A., ”The pecan nut (Carya illinoinensis) and its oil and polyphenolic fractions differentially modulate lipid metabolism and the antioxidant enzyme activities in rats fed high-fat diets”, Food Chem, 168, 529-537, 2015.
[18]  Zhang, Y., Liu, X., Han, L., Gao, X., Wang, T., “Regulation of lipid and glucose homeostasis by mango tree leaf extract is mediated by AMPK and PI3K/AKT signaling pathways”, Food Chem, 141, 2896-2905, 2013.