Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: https://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2020, 8(7), 313-319
DOI: 10.12691/jfnr-8-7-2
Open AccessArticle

Chlorogenic Acid Decreases Lipid Accumulation in 3T3-L1 Adipocytes by Modulating the Transcription Factors

Ching-Chih Liu1, Jen-Yin Chen2, 3, Chin-Chen Chu3, Shih-Ying Chen4, Heuy-Ling Chu5 and Pin-Der Duh5,

1Department of Ophthalmology Chi-Mei Medical Center, 710402, Taiwan, R.O.C.

2Department of Senior Citizen Service Management, Chia Nan University of Pharmacy and Science, 71710, Taiwan, R.O.C.

3Department of Anesthesiology, Chi-Mei Medical Center, 710402, Taiwan, R.O.C.

4Department of Health and Nutrition, Chia Nan University of Pharmacy and Science, Tainan, 71710,Taiwan, R.O.C.

5Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, 71710,Taiwan, R.O.C.

Pub. Date: July 29, 2020

Cite this paper:
Ching-Chih Liu, Jen-Yin Chen, Chin-Chen Chu, Shih-Ying Chen, Heuy-Ling Chu and Pin-Der Duh. Chlorogenic Acid Decreases Lipid Accumulation in 3T3-L1 Adipocytes by Modulating the Transcription Factors. Journal of Food and Nutrition Research. 2020; 8(7):313-319. doi: 10.12691/jfnr-8-7-2

Abstract

The aim of this study is to investigate the effect of chlorogenic acid (CA) on 3T3-L1 adipocytes and its mechanism action. CA at 1-25 μM showed no cytotoxicity to 3T3-L1 pre-adipocytes and 3T3-L1 adipocytes. CA significantly inhibited oil red O-stained material (OROSM) and intracellular triglyceride levels in 3T3-L1 adipocytes in a concentration-dependent manner. In addition, CA down-regulated the glycerol-3–phosphate dehydrogenase (GPDH) and peroxisome proliferator–activated receptor γ (PPARγ) activity. A real-time polymerase chain reaction revealed that CA inhibited the PPARγ, CCAAT/enhancer-binding protein alpha (C/EBPα), sterol regulatory element binding protein-1c (SREBP-1c), and fatty acid synthase (FAS) gene expression, which may in part account for anti-adipogenesis of CA. Thus, CA could act as a potential lipid lowering functional resource.

Keywords:
chlorogenic acid adipocytes anti-adipogenesis lipid accumulation transcription factor

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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