Article citationsMore >>

Limwachiranon, J., Huang, H., Li, L., Lin, X., Zou, L., Liu, J., Zou, Y., Aalim, H., Duan, Z., Luo, Z., “Enhancing stability and bioaccessibility of chlorogenic acid using complexation with amylopectin: A comprehensive evaluation of complex formation, properties, and characteristics”, Food Chem, 311, 125879, 2020.

has been cited by the following article:

Article

Chlorogenic Acid Decreases Lipid Accumulation in 3T3-L1 Adipocytes by Modulating the Transcription Factors

1Department of Ophthalmology Chi-Mei Medical Center, 710402, Taiwan, R.O.C.

2Department of Senior Citizen Service Management, Chia Nan University of Pharmacy and Science, 71710, Taiwan, R.O.C.

3Department of Anesthesiology, Chi-Mei Medical Center, 710402, Taiwan, R.O.C.

4Department of Health and Nutrition, Chia Nan University of Pharmacy and Science, Tainan, 71710,Taiwan, R.O.C.

5Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, 71710,Taiwan, R.O.C.


Journal of Food and Nutrition Research. 2020, Vol. 8 No. 7, 313-319
DOI: 10.12691/jfnr-8-7-2
Copyright © 2020 Science and Education Publishing

Cite this paper:
Ching-Chih Liu, Jen-Yin Chen, Chin-Chen Chu, Shih-Ying Chen, Heuy-Ling Chu, Pin-Der Duh. Chlorogenic Acid Decreases Lipid Accumulation in 3T3-L1 Adipocytes by Modulating the Transcription Factors. Journal of Food and Nutrition Research. 2020; 8(7):313-319. doi: 10.12691/jfnr-8-7-2.

Correspondence to: Pin-Der  Duh, Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, 71710,Taiwan, R.O.C.. Email: ipdduh@mail.cnu.edu.tw

Abstract

The aim of this study is to investigate the effect of chlorogenic acid (CA) on 3T3-L1 adipocytes and its mechanism action. CA at 1-25 μM showed no cytotoxicity to 3T3-L1 pre-adipocytes and 3T3-L1 adipocytes. CA significantly inhibited oil red O-stained material (OROSM) and intracellular triglyceride levels in 3T3-L1 adipocytes in a concentration-dependent manner. In addition, CA down-regulated the glycerol-3–phosphate dehydrogenase (GPDH) and peroxisome proliferator–activated receptor γ (PPARγ) activity. A real-time polymerase chain reaction revealed that CA inhibited the PPARγ, CCAAT/enhancer-binding protein alpha (C/EBPα), sterol regulatory element binding protein-1c (SREBP-1c), and fatty acid synthase (FAS) gene expression, which may in part account for anti-adipogenesis of CA. Thus, CA could act as a potential lipid lowering functional resource.

Keywords