Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2019, 7(8), 619-623
DOI: 10.12691/jfnr-7-8-10
Open AccessArticle

Capsinoids Supplementation does not Prevent Weight Gain and does not Change Lipid Profile in Wistar Rats Fed a High-Fat Diet

Ana Carolina Campi Cansian1, Caroline Bertoncini-Silva1, , Anderson Gregorio Joaquim1, Cassia Dias Machado1, Daniela Carlos2, Aline Jose Coelho Moreira Zordan1, Maria Cristina Foss Freitas3 and Vivian Marques Miguel Suen1

1LANNEM – Laboratory of Nutrition, Neuroscience and Metabolism, Department of Medicine – Division of Nutrology, Ribeirão Preto Medical School. University of São Paulo, Ribeirão Preto, Brazil

2Department of Biochemistry and Immunology, Ribeirão Preto Medical School. University of São Paulo, Ribeirão Preto, Brazil

3Department of Medicine – Division of Endocrinology and Metabolism, Ribeirão Preto Medical School – University of São Paulo, Ribeirão Preto, Brazil

Pub. Date: August 29, 2019

Cite this paper:
Ana Carolina Campi Cansian, Caroline Bertoncini-Silva, Anderson Gregorio Joaquim, Cassia Dias Machado, Daniela Carlos, Aline Jose Coelho Moreira Zordan, Maria Cristina Foss Freitas and Vivian Marques Miguel Suen. Capsinoids Supplementation does not Prevent Weight Gain and does not Change Lipid Profile in Wistar Rats Fed a High-Fat Diet. Journal of Food and Nutrition Research. 2019; 7(8):619-623. doi: 10.12691/jfnr-7-8-10


We hypothesized that capsinoids supplementation, a bioactive vanillylamines isolated from chili peppers, would exert protective effects against high-fat diet -induced weight gain via regulation of lipid, glucose and insulin profile. To test our hypothesis, twenty-four male Wistar rats were fed a standard diet, standard diet with capsinoids, high-fat diet and high-fat diet with capsinoids for 6 weeks. Capsinoids dose was 0.18 mg/kg/d. During the experiment, body weight and food intake were evaluated weekly and biochemical analyses were performed at the end of the experimental period. Only high-fat diet with capsinoids presented lower total food intake as compared to standard diet with capsinoids group (~35%). Nevertheless, there were no significant differences in weight gain between the groups. Capsinoids supplementation did not prevent retroperitoneal and epididymal fat gain on high-fat diet with capsinoids, as well did not change brown fat and liver weight. Moreover, no statistical difference was observed for high-fat diet or capsinoids supplementation on blood glucose, insulin and lipid profile. In conclusion, these results suggest that capsinoids supplementation in obesity experimental model of Wistar rats, has limited effects to prevent weight and fat gain, as well as do not regulate metabolic profile.

capsinoids metabolic profile obesity Wistar rats high-fat diet

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[1]  Upadhyay, J., Farr, O., Perakakis, N., Ghaly, W. and Mantzoros, C. “Obesity as a Disease”, The Medical Clinics of North America, 102 (1). 13-33. Jan.2018.
[2]  World Health Organization (WHO). “Fact sheet no. 311: obesity and overweight”, 2018 [online]. Available: s/detail/obesi ty-and-overweight. [Accessed Feb. 28, 2019].
[3]  Global Burden of Disease (GBD) 2015 Obesity Collaborators. “Health effects of overweight and obesity in 195 countries over 25 years”, The New England Journal of Medicine, 377 (1). 3-27. Jul.2017.
[4]  Bray, G.A., Heisel, W.E., Afshin, A., Jensen, M.D., Dietz, W.H., Long, M., Kushner, R.F., Daniels, S.R., Wadden, T.A., Tsai, A.G., Hu, F.B., Jakicic, J.M., Ryan, D.H., Wolfe, B.M. and Inge, T.H. “The science of obesity management: an endocrine society scientific statement”, Endocrine Reviews, 39 (2). 79-132. Apr.2018.
[5]  Loveman, E., Frampton, G.K., Shepherd, J., Picot, J., Cooper, K., Bryant, J., Welch, K. and Clegg, A. “The clinical effectiveness and cost-effectiveness of long-term weight management schemes for adults: a systematic review”, Health Technology Assessment (Winchester, England), 15 (2). 1-182. Jan.2011.
[6]  Akpinar, E.K., Bicer, Y. and Yildiz, C. “Thin layer drying of red pepper”, Journal of Food Engineering, 59 (1). 99-104. Aug.2009.
[7]  Sung, J. and Lee, J. “Capsicoside G, a furostanol saponin from pepper (Capsicum annuum L.) seeds, suppresses adipogenesis through activation of AMP activated protein kinase in 3T3-L1 cells”, Journal of Functional Foods, 20. 148-58. Jan.2016.
[8]  Barceloux, D.G. “Pepper and capsaicin (Capsicum and Piper species)”, Disease-a-Month: DM, 55 (6). 380-390. Jun.2009.
[9]  Ludy, M.J., Moore, G.E. and Mattes, R.D. “The effects of capsaicin and capsiate on energy balance: critical review and meta-analyses of studies in humans”, Chemical Senses, 37 (2). 103-121. Feb.2012.
[10]  Kobata. K., Sutoh, K., Todo, T., Yazawa, S., Iwai, K. and Watanabe, T. “Nordihydrocapsiate, a new capsinoid from the fruits of a nonpungent pepper, capsicum annuum”, Journal of Natural Products, 62 (2). 335-336. Feb.1999.
[11]  Kempaiah, R.K. and Srinivasan, K. “Beneficial influence of dietary curcumin, capsaicin and garlic on erythrocyte integrity in high-fat fed rats”, The Journal of Nutritional Biochemistry, 17 (7). 471-478. Jul.2006.
[12]  Otunola, G.A., Oloyede, O.B., Oladiji, A.T. and Afolayan, A.J. “Selected spices and their combination modulate hypercholesterolemia-induced oxidative stress in experimental rats”, Biological Research, 47 (1). 5. Mar.2014.
[13]  Eggink, P.M., Maliepaard, C., Tikunov, Y., Haanstra, J.P., Bovy, A.G. and Visser, R.G. “A taste of sweet pepper: Volatile and non-volatile chemical composition of fresh sweet pepper (Capsicum annuum) in relation to sensory evaluation of taste” Food Chemistry, 132 (1). 301-310. May.2012.
[14]  Kawabata, F., Inoue, N., Yazawa, S., Kawada, T., Inoue, K. and Fushiki T. “Effects of CH-19 Sweet, a non-pungent cultivar of red pepper, in decreasing the body weight and suppressing body fat accumulation by sympathetic nerve activation in humans”, Bioscience, Biotechnology and Biochemistry, 70 (12). 2824-2835. Dec.2006.
[15]  Kang, J.H., Goto, T., Han, I.S., Kawada, T., Kim, Y.M. and Yu, R. “Dietary capsaicin reduces obesity-induced insulin resistance and hepatic steatosis in obese mice fed a high-fat diet”, Obesity (Silver Spring, Md.), 18 (4). 780-787. Apr.2010.
[16]  Haramizu, S., Mizunoya, W., Masuda, Y., Ohnuki K., Watanabe, T., Yazawa, S. and Fushiki, T. “Capsiate, a nonpungent capsaicin analog, increases endurance swimming capacity of mice by stimulation of vanilloid receptors”, Bioscience, Biotechnology and Biochemistry, 70 (4). 774-781. Apr.2006.
[17]  Okumura, T., Tsukui, T., Hosokawa, M. and Miyashita, K. “Effect of caffeine and capsaicin on the blood glucose levels of obese/diabetic KK-A(y) mice”, Journal of Oleo Science, 61 (9). 515-523. 2012.
[18]  Kim, J.Y., Lee, M.S., Jung, S., Joo. H., Kim, C.T., Kim, I.H., Seo, S., Oh, S. and Kim, Y. “Anti-obesity efficacy of nanoemulsion oleoresin capsicum in obese rats fed a high-fat diet”, International Journal of Nanomedicine, 9. 301-310. 2014.
[19]  Sung, J., Jeong, H.S. and Lee, J. “Effect of the capsicoside g-rich fraction from pepper (Capsicum annuum L.). seeds on high-fat diet-induced obesity in mice”, Phytotherapy Research, 30 (11). Nov.2016.
[20]  Yeon, S.J., Kim, S.K., Kim, J.M., Lee, S.K. and Lee, C.H. “Effects of fermented pepper powder on body fat accumulation in mice fed a high-fat diet” Bioscience, Biotechnology and Biochemistry, 77 (11). 2294-2297. Nov.2013.
[21]  Iwasaki, Y., Tamura, Y., Inayoshi, K., Narukawa, M., Kobata, K., Chiba, H., Muraki, E., Tsunoda, N. and Watanabe, T. “TRPV1 agonist monoacylglycerol increases UCP1 content in brown adipose tissue and suppresses accumulation of visceral fat in mice fed a high-fat and high-sucrose diet”, Bioscience, Biotechnology and Biochemistry, 75 (5). 904-909. May.2011.
[22]  Kim, N.H. and Park, S.H. “Evaluation of green pepper (Capsicum annuum L.) juice on the weight gain and changes in lipid profile in C57BL/6 mice fed a high-fat diet”, Journal of the Scince of Food and Agriculture, 95 (1). 79-87. Jan.2015.