Amino Acid Composition of Cowpea Grains Preserved With Mixtures OF Neem (Azadirachta indica) and Moringa (Moringa oleifera) Seed Oils

Ilesanmi J.O.Y¹ and Gungula D.T.^2,

¹Department of Food Science and Technology, Modibbo Adama University of Technology, Yola, Nigeria

²Department of Crop Production and Horticulture, Modibbo Adama University of Technology, Yola, Nigeria

Cite this paper:
Ilesanmi J.O.Y and Gungula D.T.. Amino Acid Composition of Cowpea Grains Preserved With Mixtures OF Neem (Azadirachta indica) and Moringa (Moringa oleifera) Seed Oils. American Journal of Food and Nutrition. 2016; 4(6):150-156. doi: 10.12691/ajfn-4-6-2

Abstract

Dry cowpea grains are important in Africa but its storage has posed a serious challenge to farmers and researchers. Various bio-insecticides have been used for the preservation of cowpea grains in Africa, yet little effort has been made to ascertain their effects on the nutritional quality of the cowpea grains. The effect of mixture of neem and moringa seed oils used for the preservation of cowpea grains on its amino acid composition was investigated. The seed oils were extracted traditionally and applied on cowpea grain at concentrations of (2.5 µl/g and 5.0 µl/g) in ratio 1:2 and 1:3. The treated cowpea grain samples were stored for a period of 270 days. The amino acid composition of the treated cowpea grains was determined at the end of every 90 days. The result showed that the mixture of neem and moringa seed oils in ratio 1:3 and at a concentration of 5.0 µl/g significantly (P<0.05) improved the concentration of amino acids composition of the treated cowpea while no significant (P<0.05) difference in the concentration of the amino acid composition of cowpea samples treated with ratio 1:3/2.5 µl/g of the mixture of these oils. There was significant improvement in the concentrations of all the amino acids present as storage period progressed, especially at 180 days of storage. The study concludes that the mixtures of these oils especially ratios 1:3/2.5 µl/g and 1:3/5.0 µl/g may be nutritionally good for cowpea preservation.

Keywords:
cowpea preservation storage bio-insecticide amino acid composition

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References:

[1]	Carlos G (2004). Cowpea: Postharvest operations. FAO of United Nations, Rome, Italy.
[2]	Ntoukam G, Murdock LL, Shade RE (2000). Managing insect pests of cowpea in storage. Bean/Cowpea, Midcourse 2000 Research meeting, Senegal, pp.3-4, 2000, Retrieved from http://www.canr.msu.edu/ oerseas/cowpea/proceed. (July 2016)
[3]	Rao RV (1990). Natural decay resistance of neem. Wood J. Indi. Acad. Wood Sci. 21(1): 19-21.
[4]	Carino MFA (2005). Insecticidal screening of crude extracts from nine composite species and the isolation and characterization of the insecticidal fraction from Tethonic diversifolia (A. Gray) M.Sc. Thesis, University of the Philippines at Los Banos, College, Laguns. 2005.
[5]	Akou-Edi E (1983). Effect of neem seed powder and oil on Tribolium confusum and Sitophilus zeamais Natural Pesticides from the tree (Azadirachta indica A. Juss) and other tropical plants. Proceedings of the 2nd International Neem Conference, Ravischholzhaueen, Fed. Rep. of Germany 25-28 May, 1983. Pp 445-451. 1983.
[6]	Cheftel JC, Cuq JL, Lorient D (1985). Protéines alimentaires. Biochimie–propriétés fonctionnelles – valeur nutritionnelle–modifications chimiques. Paris: Technique et documentation – Lavoisier, pp. 1-295.
[7]	Abbey BW (2006). Bridging the protein gap with what you have. University of Port Harcourt Inaugural Lecture Series, No. 48, Port Harcourt, Nigeria. P.25. 2006
[8]	Afoakwa EO, Aidoo PR (2007) Changes in Souring Development, Nutritional and Functional Properties during Fermentation of Cowpea-Fortified Nixtamalized Maize. Intl J. of Food Engr. 2(3) Available at: http://works.bepress.com/emmanueloheneafoakwa/2/ (July 2016).
[9]	Bliss FA (1975). Cowpeas in Nigeria. In: Milner M (ed.). Proceedings of a symposium on Nutritional Improvement of Food Legumes by Breeding 3-5 July 1972. New York, United Nations Protein Advisory group, 151-158. 1975.
[10]	FAO 1992. Human nutrition in the developing world. http://www.fao.org/docrep/w0073e/w0073e03.htm (July 2016)
[11]	Oyeyinka SA, Oyeyinka AT, Karim OR, Kayode RMO, Balogun MA, Balogun OA (2013). Quality attributes of weevils (Callosobruchus maculatus) infested cowpea (Vigna unguiculata) products. Nig. J. Agric. Food Environ. 9(3): 16-22. 2013
[12]	Mitchell DC, Lawrence FR, Hartman TJ, Curran JM (2009). Consumption of dry beans, peas, and lentils could improve diet quality in the US population. J. Am. Diet Assoc. 109(5): 909-13. 2009.
[13]	Anderson JA, Alcock AW (2013). Storage of cereal grains and their products. Fourth edition. Pp.515
[14]	Ilesanmi JOY, Gungula DT (2010). Preservation of cowpea [Vigna unguiculata (L.)] Walp grains against Cowpea Bruchids (Callosobruchus maculatus) using neem and moringa seed oils. Int. J. Agron. 2010: 1687-8159.
[15]	Benitez LV (1989). Amino acid and fatty acid profiles in aquaculture nutrition studies. In: SS De Silva (ed.). Fish nutrition research in Asia. Proceedings of the Third Asian Fish Nutrition Network Meeting. Asian Fish Society Special Publication. 4, 166 p. 1989.
[16]	Block RJ, Mitchell HH (1946) The Correlation of the Amino Acid Composition of Proteins with Their Nutritive Value. Nutrition Abstracts & Reviews, 16, 249-278.
[17]	FAO/WHO (1973) Energy and Protein Requirements. Report of a Joint FAO/WHO Adhoc Expert Committee. FAO Nutritional Meeting Report Series No. 52, Technical Report Series No. 522 FAO of the United Nation, Rome, Italy.
[18]	FAO/WHO (1991) Protein Quality Evaluation: Report of the Joint FAO/WHO Expert Consultation, FAO Food and Nutrition Paper 51. Rome, Italy.
[19]	Oser BL (1959) An Integrated Essential Amino Acid Index for Predicting the Biological Value of Proteins. In: Alba-nese, A.A., Ed., Amino Acid Nutrition, Academic Press, New York, 295-311.
[20]	Hidvegi M, Bekes F (1984) Mathematical Modeling of Protein Nutritional Quality from Amino Acid Composi-tion. In: Lazity, R. and Hidvegi, M., Eds., Proceedings of the International Association for Cereal Chemistry Sympo-sium, Academic Kiado, Budapest, 205-286.
[21]	Alsmeyer RH, Cunningham AE, Happich, ML (1974) Equations Predict PER from Amino Acid Analysis. Food Tech., 28, 34-38.
[22]	SAS Institute (2011). The SAS system for Windows. Release 9.2. SAS Inst., Cary, NC.
[23]	Mune MAM, Minka SR, Mbome IL (2013). Chemical composition and nutritional evaluation of a cowpea protein concentrate. Global Adv. Res. J. Food Sci. Technol.2 (3): 035-043.
[24]	Nell FJ, Siebrits FK, Hayes JP (1992). Studies on nutritive value of cowpeas (Vigna unguiculata). S. Afr. J. Animal Science 22 (5) p 157-160. 1992
[25]	Aremu MO, Olaofe O, Akintayo TE (2006). A comparative study on the chemical and amino acid composition of some Nigerian under-utilized legume flours. Pak. J. Nutr. 5(1): 34-38.
[26]	Duke JA (1983). Handbook of energy crops. 1983. Retrieved from http://www.hort.purdue.edu/newcrop/duke_energy/moringa_oleifera.htmi (Sept 2006).
[27]	Fahey JW (2005). Moringa oleifera: A review of the medical evidence for its nutritional, therapeutic, and prophylactic properties. Tree for Life J. 1: 15. 2005. www.TFL. journal.org. Retrieved 7th October 2010.
[28]	El-Adawy TA, Rahma EH, El-Bedawey AA, Gafar AF (2001). Nutritional potential and functional properties of sweet and bitter lupin seed protein isolates. Food Chem. 74: 455-462.
[29]	Chavan UD, McKenzie DB Shahidi F (2001). Functional properties of protein isolates from beach pea (Lathyrus maritimus L). Food Chem. 74. 177-187.