Vitali Kalantaryan, Radik Martirosyan, Yuri Babayan, Lusine Nersesyan, Hrachya Stepanyan
American Journal of Medical and Biological Research. 2014, 2(1), 18-25DOI:
Abstract: Unlike now widely used traditional methods of treatment of tumors by means of ionizing radiation and the chemotherapy, the method of the use of low intensity electromagnetic fields (EMF) is non-ionizing and non-invasive and hence is completely deprived of any harmful side effects. The present study was undertaken to investigate whether low-intensity EMFs can suppress tumoral cells growth in vivo without cytostatic agents. The course of influence of EMFs started 3 days before transplantation in order to raise activity of the animals’ immune system. On the fourth day animals were injected by sarcoma-37 and daily exposure was continued during 15 days. For study of the effect of irradiation on the secondary structure of DNA, in the experiments DNA isolated from the liver of healthy mice (hDNA) as well as from the tumor sarcoma 37 (tDNA) was used. After 15 sessions of exposure without cytostatic drugs, at animals of the irradiated 0,5 hour was observed an inhibition of tumor growth by 33.5% compared with a control group and a sharp suppression of the level of DNA-methylation in 2.1 times. The tDNA has the high level of methylation (4.7 mol%), which after 0.5 hour daily exposure becomes (2.2 mol%) close to the corresponding value for hDNA (1.9 mol%). Differential melting curves (DMC) of tDNA are shifted relatively DMC of the hDNA to lower temperatures, and in the DMC of tDNA the additional peaks in the 52-60ºC range are appeared, which are absent for DMC of liver hDNA. The obtained results are correlated with the spectrophotometric data. Under the influence of EMFs the values of temperature and interval of melting of tDNA are changed and approach to the corresponding values of hDNA. Presented preliminary results have demonstrated the potential clinical application of low power EMFs for clinical oncology in the treatment of malignancies. The changes of physical-chemical properties of tumoral and healthy DNA under amplitude modulated radiation at 64.5 GHz and possible mechanisms of these changes have been investigated and discussed.