Abstract: Objective: Aim of our study is to investigate physical and chemical structure and properties of body lipids at various anatomical sites. Trial design: A pilot physical and chemical descriptive experimental trial in vitro. Methods and Participants: Adipose tissue in the amount of 252 samples from 36 individuals (17 dead females) at autopsy. The subjects had died from various injuries and were between 36-60 years old. Interventions: Chemical groups and compounds were studied on infrared spectrometry with software, and atomic adsorptive analysis on spectrometer. Elemental chemical analysis of lipids different localization carried out. Research Subject: Chemical elements and compounds. Results: The highest levels of saturated fatty acids and almost all chemical groups and compounds analysed are found in dense atherosclerotic plaque (AP). In those samples, relatively more compounds containing metabolic products were identified (P < 0.05, n = 252). Dense AP contains relatively more saturated and branched hydrocarbon chains, and they have the largest quantities of organic and inorganic elements and compounds in their structure. Conclusions: Human body lipids, especially dense AP, serve as a depot for various organic substances. Once having been formed, the AP has its own pathophysiological role in adsorption of redundant waste products.

Abstract: Aim: This study was carried out to assess the recovery rate of Enterococcus faecalis count after disinfection of the root canals with Er,Cr:YSGG laser. Methodology: Freshly extracted 40 human teeth of single straight root canals were prepared to a size 50; these specimens were sterilized and then inoculated with E.faecalis suspension and incubated for 48 hrs. They were randomly allocated to three treatments and one control negative group. The infected root canals underwent laser treatment. Then samples were collected immediately (after irradiation of root canal) and after 7 days of irradiation, after that surviving of bacteria were checked by counting the colony-forming units (CFU). Results: The results indicated that the Er,Cr:YSGG laser was capable of significant reduction of E.faecalis in the infected root canal at all selected output powers 0.75W, 1W and 1.5W as well as there was significant antibacterial differences among these powers. In addition the results showed increased in the number of bacterial colonies in the second microbiological sampling compared with the first microbiological sampling, The bacterial recovery decreased when laser irradiation power increased. The best result for the laser that showing less number of E.faecalis after 7 days of irradiation at 1.5W. Conclusions: This study recognized the disinfection ability of Er,Cr:YSGG laser against the most resistant bacteria that is associated with endodontic infection.

Abstract: Unlike now widely used traditional methods of treatment of tumors by means of ionizing radiation and the chemotherapy, the method of the use of low intensity electromagnetic fields (EMF) is non-ionizing and non-invasive and hence is completely deprived of any harmful side effects. The present study was undertaken to investigate whether low-intensity EMFs can suppress tumoral cells growth in vivo without cytostatic agents. The course of influence of EMFs started 3 days before transplantation in order to raise activity of the animals’ immune system. On the fourth day animals were injected by sarcoma-37 and daily exposure was continued during 15 days. For study of the effect of irradiation on the secondary structure of DNA, in the experiments DNA isolated from the liver of healthy mice (hDNA) as well as from the tumor sarcoma 37 (tDNA) was used. After 15 sessions of exposure without cytostatic drugs, at animals of the irradiated 0,5 hour was observed an inhibition of tumor growth by 33.5% compared with a control group and a sharp suppression of the level of DNA-methylation in 2.1 times. The tDNA has the high level of methylation (4.7 mol%), which after 0.5 hour daily exposure becomes (2.2 mol%) close to the corresponding value for hDNA (1.9 mol%). Differential melting curves (DMC) of tDNA are shifted relatively DMC of the hDNA to lower temperatures, and in the DMC of tDNA the additional peaks in the 52-60ºC range are appeared, which are absent for DMC of liver hDNA. The obtained results are correlated with the spectrophotometric data. Under the influence of EMFs the values of temperature and interval of melting of tDNA are changed and approach to the corresponding values of hDNA. Presented preliminary results have demonstrated the potential clinical application of low power EMFs for clinical oncology in the treatment of malignancies. The changes of physical-chemical properties of tumoral and healthy DNA under amplitude modulated radiation at 64.5 GHz and possible mechanisms of these changes have been investigated and discussed.

Abstract: Aim: Herbs play a significant role in pharmaceutical industries as life saving drugs. This calls for the discovery of new antimicrobial compounds with diverse chemical structures and novel mechanisms of action. The present study focuses on imparting antimicrobial finish to the denim fabric using natural herbal extracts. Methodology: Three herbs, Ricinus communis, Senna auriculata and Euphorbia hirta were screened for their antimicrobial efficiency by finishing the methanolic extracts of these herbs on the denim fabric by qualitative test method EN ISO 20645. The combinatorial antimicrobial efficiency (1:3:2) of these extracts were studied and confirmed quantitatively by performing AATCC 100 test method. The antifungal activity was tested according to AATCC 30 test method. Results: The combinatorial ratio (1:3:2) showed 98% bacterial reduction against Escherichia coli and 99% against Staphylococcus aureus. Same ratio also showed 100% mycelial reduction against Aspergillus niger. Conclusion: The combinatorial antibacterial activities of the selected three herbs showed excellent results compared to the individual finishing.

Abstract: Although in experimental psychology the fixation/saccadic sequence is a main topic of debate, so far in the clinical ophthalmological field such an argument has raised relatively few interest. Nevertheless, studying the possible alterations of the saccadic rate and of the positioning of fixations when analyzing a visual scene may illuminate on the source of reading difficulty in a variety of patients, like dyslexic children or those affected by hemianopia as well as central visual field impairment. Recently, new light has been shed on the saccadic/fixation dynamics, both in the temporal and spatial domain. In addition, according to recent findings a visuoperceptive distortion could potentially affect the saccades/fixation sequence, making the matter much more complex than expected. The aim of this article is therefore to describe the saccadic/fixation seesaw under the temporal and spatial perspective and to disclose the possible influence of visuoperceptive impairments.