American Journal of Microbiological Research
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American Journal of Microbiological Research. 2013, 1(4), 92-97
DOI: 10.12691/ajmr-1-4-5
Open AccessArticle

Combined Application of Biological-Photocatalytic Process in Degradation of Reactive Black Dye: An Excellent Outcome

Maulin P Shah1,

1Industrial Waste Water Research Laboratory, Applied & Environmental Microbiology Lab, Enviro Technology Limited (CETP), Ankleshwar, Gujarat, India

Pub. Date: December 05, 2013

Cite this paper:
Maulin P Shah. Combined Application of Biological-Photocatalytic Process in Degradation of Reactive Black Dye: An Excellent Outcome. American Journal of Microbiological Research. 2013; 1(4):92-97. doi: 10.12691/ajmr-1-4-5


In the present study, the decolorization and degradation of Reactive Black 5 azo dye was investigated by biological, photocatalytic (UV/TiO2) and combined processes. An application of Pseudomonas aeruginosa ETL-2211 in treatment of the synthetic medium containing RB5 indicated complete decolorization of the dye with 200 mg/L in less than 24 h. Degradation of the aromatic rings, resulting from the destruction of the dye, did not occur during the biological treatment. Mineralization of 50 mg/L RB5 solution was obtained after 80 min by photocatalytic process (in presence of 0.2 g/L TiO2). COD (chemical oxygen demand) was not detectable after complete decolorization of 50 mg/L RB5 solution. However, photocatalytic process was not effective in the removal of the dye at high concentrations (≥200 mg/L). With 200 mg/L concentration, 74.9% of decolorization was achieved after 4 h illumination under photocatalytic process and the absorbance peak in UV region (attributed to aromatic rings) was not completely removed. A two-step treatment process, namely, biological treatment by bacteria followed by photocatalytic degradation, was also assessed. In the combined process (with 200 mg/L RB5), absorbance peak in UV region significantly disappeared after 2 h illumination and about 60% COD removal was achieved in the biological step. It is suggested that the combined process is more effective than the biological and photocatalytic treatments in the remediation of aromatic rings.

azo dyes Pseudomonas aeruginosa combined wastewater treatment

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