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Lucas MS, Dias AA, Sampaio A, Amaral C, Peres JA: Dagradation of a textile reactive azo dye by a combined chemical-biological process: Fenton, s reagent-yeast. Water Res 2007, 41:1103-1109.

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Article

Combined Application of Biological-Photocatalytic Process in Degradation of Reactive Black Dye: An Excellent Outcome

1Industrial Waste Water Research Laboratory, Applied & Environmental Microbiology Lab, Enviro Technology Limited (CETP), Ankleshwar, Gujarat, India


American Journal of Microbiological Research. 2013, Vol. 1 No. 4, 92-97
DOI: 10.12691/ajmr-1-4-5
Copyright © 2013 Science and Education Publishing

Cite this paper:
Maulin P Shah. Combined Application of Biological-Photocatalytic Process in Degradation of Reactive Black Dye: An Excellent Outcome. American Journal of Microbiological Research. 2013; 1(4):92-97. doi: 10.12691/ajmr-1-4-5.

Correspondence to: Maulin  P Shah, Industrial Waste Water Research Laboratory, Applied & Environmental Microbiology Lab, Enviro Technology Limited (CETP), Ankleshwar, Gujarat, India. Email: shahmp@uniphos.com

Abstract

In the present study, the decolorization and degradation of Reactive Black 5 azo dye was investigated by biological, photocatalytic (UV/TiO2) and combined processes. An application of Pseudomonas aeruginosa ETL-2211 in treatment of the synthetic medium containing RB5 indicated complete decolorization of the dye with 200 mg/L in less than 24 h. Degradation of the aromatic rings, resulting from the destruction of the dye, did not occur during the biological treatment. Mineralization of 50 mg/L RB5 solution was obtained after 80 min by photocatalytic process (in presence of 0.2 g/L TiO2). COD (chemical oxygen demand) was not detectable after complete decolorization of 50 mg/L RB5 solution. However, photocatalytic process was not effective in the removal of the dye at high concentrations (≥200 mg/L). With 200 mg/L concentration, 74.9% of decolorization was achieved after 4 h illumination under photocatalytic process and the absorbance peak in UV region (attributed to aromatic rings) was not completely removed. A two-step treatment process, namely, biological treatment by bacteria followed by photocatalytic degradation, was also assessed. In the combined process (with 200 mg/L RB5), absorbance peak in UV region significantly disappeared after 2 h illumination and about 60% COD removal was achieved in the biological step. It is suggested that the combined process is more effective than the biological and photocatalytic treatments in the remediation of aromatic rings.

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