Regeneration, Nutritional Values, and Antioxidants in Excised Adventitious Shoot of Radish Affected by Dark Treatment

Hyun-Sug Choi¹, Se Ji Jang², Hye Ji Park², Young Beom Yun² and Yong In Kuk^2,

¹Department of Horticulture, Catholic University of Daegu, Gyeongsan, Gyeongsangbuk-do 712-702, Republic of Korea

²Department of Development in Oriental Medicine Resources, Sunchon National University, Suncheon, Jeollanam-do, Republic of Korea

Cite this paper:
Hyun-Sug Choi, Se Ji Jang, Hye Ji Park, Young Beom Yun and Yong In Kuk. Regeneration, Nutritional Values, and Antioxidants in Excised Adventitious Shoot of Radish Affected by Dark Treatment. Journal of Food and Nutrition Research. 2015; 3(6):365-370. doi: 10.12691/jfnr-3-6-2

Abstract

The study was to evaluate regeneration, mineral nutrients, and antioxidative activities of adventitious shoot in tuberous root radish (Raphanus sativus L.) grown under light and dark conditions in a controlled growth chamber. Small pieces of top of root radish, including adventitious shoots, were detached from mother radish roots (1^st cut) and grown for 22 days (22 DAC₁), which then re-cut (2^nd cut) and re-grown for 23 days (23 DAC₂). Fresh weight was heavier on plants grown under the dark condition (6.2 g) at 22 DAC₁ compared to the light condition (5.2 g) but was not significantly different between the treatment conditions at 23 DAC₂. Shoot length was significantly extended by the dark condition during the experimental period. Leaf concentrations of Ca, Mg, Na, and Mn were significantly increased by the light condition, with high leaf concentrations of Fe, Zn, and Cu observed on the dark condition. Proline of total amino acids was highly increased by the light condition (126.8 mg g^-1) compared to dark condition (16.3 mg g^-1), but other total amino acid concentrations were varied between the treatment conditions. Fructose and sucrose were increased by dark and light conditions, respectively. Dark condition reduced scavenging activity (9.7%), total phenolic (12.2 mg g^-1) and flavonoid compounds (0.05 mg g^-1) compared to the light condition (scavenging activity; 49.3%, total phenolic 43.8 mg g^-1 and flavonoid compounds; 0.21 mg g^-1) at 22 DAC₁, which was also observed at 23 DAC₂. Excised adventitious shoot of radish grown under the dark condition could be useful propagation technique for convenient consumption at all times and the physicochemical values to some degree.

Keywords:
antioxidant dark nutrient radish regeneration

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References:

[1]	Lotter, D.W, “Organic agriculture,” Journal of Sustainable Agriculture, 21. 59-128. 2003.
[2]	Guh, J.O. and Kuk, Y.I, “Weeding hypothesis on direct seeding rice field as applied by the old firing and water dressing method,” Korean Journal of Weed Science, 31. 1-7. 2011.
[3]	Poorter, H. and Nagel, O, “The role of biomass allocation in the growth response of plants to different levels of light, CO2, nutrients and water: a quantitative review,” Functional Plant Biology, 27. 595-607. 2000.
[4]	Yun, Y.B, Ryu, D.K., Jang, S.J., Kwon, O.D., Choi, H.S., Jung, H.I. and Kuk, Y.I, “Growth, physiochemical components, and antioxidative activities of Chinese chive (Allium tuberosum Rottler) grown under light and dark conditions,” Korean Journal of International Agriculture, 24. 331-336. 2012.
[5]	Choi, S.T., Lee, J.T. and Park, W.C, “Dormancy physiology, softening culture and evaluation of nutrition value in the Ulrung-native Allium victorialis var. platyphyllum,” Journal of the Korean Society for Applied Biological Chemistry, 36. 495-501. 1993.
[6]	Woo, S.J. and Ryoo, S.S, “Preparation methods for atomic absorption and spectrophotometry of food samples,” Korean Journal of Food Science and Technology, 15. 225-231. 1983.
[7]	Ohara, I. and Shujiro, A, “Comparison of protein precipitants for the determination of free amino acids in plasma,” Agricultural and Biological Chemistry, 43. 1473-1478. 1979.
[8]	Wilson, A.M., Work, T.M., Bushway, A.A. and Bushway, R.J, “HPLC determination of fructose, glucose, and sucrose in potatoes,” Journal of Food Science, 46. 300-301. 1981.
[9]	Colebrook, E.H., Thomas, S.G., Phillips, A.L. and Hedden, P, “The role of gibberellin signaling in plant responses to abiotic stress,” Journal of Experimental Biology, 217. 67-75. 2014.
[10]	Tamimi, S. and Firn, R.D, “The basipetal auxin transport system and the control of cell elongation in hypocotyls,” Journal of Experimental Botany, 36. 955-962. 1985.
[11]	Pill, W.G. and Lambeth, V.N, “Effects of NH4 and NO3 nutrition with and without pH adjustment on tomato growth, ion composition, and water relations,” Journal of the American Society for Horticultural Science, 102. 78-81. 1977.
[12]	Epstrin, E, “Mineral nutrition of plants: mechanisms of uptake and transport,” Annual Review of Plant Physiology, 7. 1-24. 1956.
[13]	Pérez-Alfocea, F., Estan, M.F., Caro, M. and Guerrier, G, “Osmotic adjustment in Lycopersicon esculentum and L. pennellii under NaCl and polyethylene glycol 6000 iso-osmotic stresses,” Physiologia Plantarum, 87. 493-498. 1993.
[14]	Claussen, W., Brückner, B., Krumbein, A. and Lenz, F, “Long-term response of tomato plants to changing nutrient concentration in the root environment—the role of proline as an indicator of sensory fruit quality,” Plant Science, 171. 323-331. 2006.
[15]	McArtney, S.J. and Ferree, D.C, “Shading effects on dry matter partitioning, remobilization of stored reserves and early season vegetative development of grapevines in the year after treatment,” Journal of the American Society for Horticultural Science, 124. 591-597. 1999.
[16]	Geiger, D.R. and Batey, J.W, “Translocation of 14C sucrose in sugar beet during darkness,” Plant Physiology, 42. 1743-1749. 1967.
[17]	Cartea, M.E. and Velasco, P, “Glucosinolates in Brassica foods: bioavailability in food and significance for human health,” Phytochemistry Reviews, 7. 213-229. 2008.
[18]	Wang, S.Y., Chen, C.T. and Wang, C.Y, “The influence of light and maturity on fruit quality and flavonoid content of red raspberries,” Food Chemistry, 112. 676-684. 2009.
[19]	Kwon, J.W., Park, J.H., Kwon, K.S., Kim, D.S., Jeong, J.B., Lee, H.K., Sim, Y.E., Kim, M.S., Young, J.Y., Chung, G.Y. and Jeong, H.J, “Effect of shading practices on the chemical compounds and antioxidant in Aruncus diocicus,” Korean Journal of Plant Resources, 19. 1-7. 2006.
[20]	Chon, S.U, “Shading effect on plant growth and physiological activity of Youngia sonchifolia growth in plastic house,” Korean Journal Weed Science, 30. 215-224. 2010.
[21]	Jin, P., Yao, D., Xu, F., Wang, H. and Zheng, Y, “Effect of light on quality and bioactive compounds in postharvest broccoli florets,” Food Chemistry, 172. 705-709. 2015.
[22]	Samuoliene, G., Sirtautas, R., Brazaityte, A. and Duchovskis, P, “LED lighting and seasonality effects antioxidant properties of baby leaf lettuce,” Food Chemistry, 134. 1494-1499. 2012.