High Fiber Diet Suppression of TLR4 and NFκβ Gene Expression Correlated with TNF-α and IL-6 Levels in Hypertriglyceridemia Rats

I Gusti Ayu Nyoman Danuyanti^{1, 2,} , Arta Farmawati³ and Sunarti³

¹Doctoral Program, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia

²Departement of Medical Laboratory Technology, Politeknik Kesehatan Mataram, Mataram, Indonesia

³Department of Biochemistry, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia

Cite this paper:
I Gusti Ayu Nyoman Danuyanti, Arta Farmawati and Sunarti. High Fiber Diet Suppression of TLR4 and NFκβ Gene Expression Correlated with TNF-α and IL-6 Levels in Hypertriglyceridemia Rats. Journal of Food and Nutrition Research. 2018; 6(12):725-730. doi: 10.12691/jfnr-6-12-2

Abstract

Hypertriglyceridemia causes hypoxia and increases the release of free fatty acids in adipose tissue through lipolysis which stimulates an increase in TLR4 and NFκβ levels as well as TNF-α and IL-6 secretion. The high fiber diet had a suppressing effect against TLR4 and NFκβ gene expression and decreased TNF-α and IL-6 levels. This study evaluated the effect of high fiber diet (P1= 1.04 g content of fiber/rat/day, P2= 2.07 g content of fiber /rat/day and P3= 3.11 g content of fiber/rat/day) on TNF-α and IL-6 levels as well as expression of TLR4 and NFκβ in rats induced by high fat and fructose diet for 6 weeks. The findings indicate that the administration of high fiber diet to the hypertriglyceridemia rats could control body weight gain and significantly reduce TNF-α and IL-6 levels (p<0.05) lower than the hypertriglyceridemia groups when compared before treatment. In addition, the treatment of high fiber diet resulted in suppressing the expression of TLR4 and NFκβ gene (p<0.05) when compared with the normal and hypertriglyceridemia groups. The suppression of TLR4 and NFκβ were correlated with TNF-α and IL-6 levels. In conclusion, the administration of the high fiber diet to the hypertriglyceridemia rats was able to decrease TNF-α and IL-6 levels possibly by suppression of TLR4 and NFκB gene expression.

Keywords:
TLR4 NFκβ TNF-α IL-6 hypertriglyceridemia high fiber diet

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