Journal of Food and Nutrition Research
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Journal of Food and Nutrition Research. 2018, 6(11), 706-709
DOI: 10.12691/jfnr-6-11-5
Open AccessArticle

Inhibitory Effect of Hydroxycitrate on Calcium Oxalate Crystal Formation in a Drosophila Model

Wen-Chi Chen1, 2, , Huey-Yi Chen1, 2, Wei-Yong Lin1, 2, You-Rong Yang2, Ming-Yen Tsai1, 3 and Yung-Hsiang Chen1, 4,

1Graduate Institute of Integrated Medicine, China Medical University, Taichung 404, Taiwan

2Departments of Urology, Obstetrics and Gynecology, Medical Research, China Medical University Hospital, Taichung 404, Taiwan

3Department of Chinese Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan

4Departments of Urology, Obstetrics and Gynecology, Medical Research, China Medical University Hospital, Taichung 404, Taiwan;Department of Psychology, College of Medical and Health Science, Asia University, Taichung 41354, Taiwan

Pub. Date: December 13, 2018

Cite this paper:
Wen-Chi Chen, Huey-Yi Chen, Wei-Yong Lin, You-Rong Yang, Ming-Yen Tsai and Yung-Hsiang Chen. Inhibitory Effect of Hydroxycitrate on Calcium Oxalate Crystal Formation in a Drosophila Model. Journal of Food and Nutrition Research. 2018; 6(11):706-709. doi: 10.12691/jfnr-6-11-5


In this study, we aimed to compare the effects of hydroxycitrate (HCA) and potassium citrate (PC) on the prevention and treatment of calcium oxalate (CaOx) stones in a fruit fly model. Drosophila melanogaster was used as an insect model of lithogenesis. The lithogenic agent used was 0.25% ethylene glycol. For determining the preventive effects, 2% PC and 2% HCA were added along with the lithogenic agent at the start of experiment. For determining the treatment effects, the lithogenic agent was added at the start of experiment to induce crystal formation, and 2% PC and 2% HCA were added from the third week. After 3 weeks, the Malpighian tubules of Drosophila were observed under polarized light microscopy, and the results were calculated. The preventive effect on the formation of CaOx in PC group was 9.38 ± 4.42% and in HCA group was 4.51 ± 3.85%. The treatment effect of PC was 56.90 ± 20.43% and that of HCA was 39.09 ± 15.36%. HCA has both preventive and treatment effects on the formation of CaOx crystals in the Malpighian tubules of Drosophila, and the effects were better than those of PC.

calcium oxalate citrate hydroxycitrate drosophila melanogaster Malphigian tubules

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