Inhibitory Effect of Hydroxycitrate on Calcium Oxalate Crystal Formation in a Drosophila Model

Wen-Chi Chen^{1, 2,} , Huey-Yi Chen^{1, 2}, Wei-Yong Lin^{1, 2}, You-Rong Yang², Ming-Yen Tsai^{1, 3} and Yung-Hsiang Chen^{1, 4,}

¹Graduate Institute of Integrated Medicine, China Medical University, Taichung 404, Taiwan

²Departments of Urology, Obstetrics and Gynecology, Medical Research, China Medical University Hospital, Taichung 404, Taiwan

³Department of Chinese Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan

⁴Departments of Urology, Obstetrics and Gynecology, Medical Research, China Medical University Hospital, Taichung 404, Taiwan;Department of Psychology, College of Medical and Health Science, Asia University, Taichung 41354, Taiwan

Cite this paper:
Wen-Chi Chen, Huey-Yi Chen, Wei-Yong Lin, You-Rong Yang, Ming-Yen Tsai and Yung-Hsiang Chen. Inhibitory Effect of Hydroxycitrate on Calcium Oxalate Crystal Formation in a Drosophila Model. Journal of Food and Nutrition Research. 2018; 6(11):706-709. doi: 10.12691/jfnr-6-11-5

Abstract

In this study, we aimed to compare the effects of hydroxycitrate (HCA) and potassium citrate (PC) on the prevention and treatment of calcium oxalate (CaOx) stones in a fruit fly model. Drosophila melanogaster was used as an insect model of lithogenesis. The lithogenic agent used was 0.25% ethylene glycol. For determining the preventive effects, 2% PC and 2% HCA were added along with the lithogenic agent at the start of experiment. For determining the treatment effects, the lithogenic agent was added at the start of experiment to induce crystal formation, and 2% PC and 2% HCA were added from the third week. After 3 weeks, the Malpighian tubules of Drosophila were observed under polarized light microscopy, and the results were calculated. The preventive effect on the formation of CaOx in PC group was 9.38 ± 4.42% and in HCA group was 4.51 ± 3.85%. The treatment effect of PC was 56.90 ± 20.43% and that of HCA was 39.09 ± 15.36%. HCA has both preventive and treatment effects on the formation of CaOx crystals in the Malpighian tubules of Drosophila, and the effects were better than those of PC.

Keywords:
calcium oxalate citrate hydroxycitrate drosophila melanogaster Malphigian tubules

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References:

[1]	G. Giacalone, and V. Chiabrando, “Effect of different treatments with calcium salts on sensory quality of fresh-cut apple,” Journal of Food and Nutrition Research, 52 (2). 79-86. 2013.
[2]	M. B. Kralj, M. Podrazka, B. Krawczyk, R. P. Mikus, K. Jarni, and P. Trebse, ““Raw food” diet: the effect of maximal temperature (46 +/- 1 degrees C) on aflatoxin B-1 and oxalate contents in food,” Journal of Food and Nutrition Research, 56 (3). 277-282. 2017.
[3]	M. Carvalho, B. O. Erbano, E. Y. Kuwaki, H. P. Pontes, J. Liu, L. H. Boros, M. O. Asinelli, and C. P. Baena, “Effect of potassium citrate supplement on stone recurrence before or after lithotripsy: systematic review and meta-analysis,” Urolithiasis, 45 (5). 449-455. 2017.
[4]	S. K. Choi, Y. G. Kim, K. H. Yoo, D. G. Lee, G. E. Min, and H. L. Lee, “Hyperkalemic cardiac arrhythmia resulting from short-term ingestion of potassium citrate for the management of ureter stones,” Urolithiasis, 44 (3). 283-284. 2016.
[5]	Y. Song, N. Hernandez, J. Shoag, D. S. Goldfarb, and B. H. Eisner, “Potassium citrate decreases urine calcium excretion in patients with hypocitraturic calcium oxalate nephrolithiasis,” Urolithiasis, 44 (2). 145-148. 2016.
[6]	F. L. Coe, J. H. Parks, and J. R. Asplin, “The pathogenesis and treatment of kidney stones,” N Engl J Med, 327 (16). 1141-1152. 1992.
[7]	R. L. Ryall, R. M. Harnett, and V. R. Marshall, “The effect of urine, pyrophosphate, citrate, magnesium and glycosaminoglycans on the growth and aggregation of calcium oxalate crystals in vitro,” Clin Chim Acta, 112 (3). 349-356. 1981.
[8]	J. Chung, I. Granja, M. G. Taylor, G. Mpourmpakis, J. R. Asplin, and J. D. Rimer, “Molecular modifiers reveal a mechanism of pathological crystal growth inhibition,” Nature, 536 (7617). 446-450. 2016.
[9]	Y. H. Chen, H. P. Liu, H. Y. Chen, F. J. Tsai, C. H. Chang, Y. J. Lee, W. Y. Lin, and W. C. Chen, “Ethylene glycol induces calcium oxalate crystal deposition in Malpighian tubules: a Drosophila model for nephrolithiasis/urolithiasis,” Kidney Int, 80 (4). 369-377. 2011.
[10]	W. C. Chen, H. Y. Chen, P. C. Liao, S. J. Wang, M. Y. Tsai, Y. H. Chen, and W. Y. Lin, “Toward a new insight of calcium oxalate stones in Drosophila by micro-computerized tomography,” Urolithiasis, 46 (2). 149-155. 2018.
[11]	W. C. Chen, W. Y. Lin, H. Y. Chen, C. H. Chang, F. J. Tsai, K. M. Man, J. L. Shen, and Y. H. Chen, “Melamine-induced urolithiasis in a Drosophila model,” J Agric Food Chem, 60 (10). 2753-2757. 2012.
[12]	M. R. Greenwood, M. P. Cleary, R. Gruen, D. Blase, J. S. Stern, J. Triscari, and A. C. Sullivan, “Effect of (-)-hydroxycitrate on development of obesity in the Zucker obese rat,” Am J Physiol, 240 (1). E72-78. 1981.
[13]	M. Leonhardt, B. Hrupka, and W. Langhans, “Effect of hydroxycitrate on food intake and body weight regain after a period of restrictive feeding in male rats,” Physiol Behav, 74 (1-2). 191-196. 2001.
[14]	A. C. Sullivan, J. G. Hamilton, O. N. Miller, and V. R. Wheatley, “Inhibition of lipogenesis in rat liver by (-)-hydroxycitrate,” Arch Biochem Biophys, 150 (1). 183-190. 1972.
[15]	C. Sullivan, and J. Triscari, “Metabolic regulation as a control for lipid disorders. I. Influence of (--)-hydroxycitrate on experimentally induced obesity in the rodent,” Am J Clin Nutr, 30 (5). 767-776. 1977.
[16]	J. A. Watson, M. Fang, and J. M. Lowenstein, “Tricarballylate and hydroxycitrate: substrate and inhibitor of ATP: citrate oxaloacetate lyase,” Arch Biochem Biophys, 135 (1). 209-217. 1969.
[17]	M. Shara, S. E. Ohia, T. Yasmin, A. Zardetto-Smith, A. Kincaid, M. Bagchi, A. Chatterjee, D. Bagchi, and S. J. Stohs, “Dose- and time-dependent effects of a novel (-)-hydroxycitric acid extract on body weight, hepatic and testicular lipid peroxidation, DNA fragmentation and histopathological data over a period of 90 days,” Mol Cell Biochem, 254 (1-2). 339-346. 2003.
[18]	M. Leonhardt, and W. Langhans, “Hydroxycitrate has long-term effects on feeding behavior, body weight regain and metabolism after body weight loss in male rats,” J Nutr, 132 (7). 1977-1982. 2002.
[19]	E. M. Kovacs, M. S. Westerterp-Plantenga, and W. H. Saris, “The effects of 2-week ingestion of (--)-hydroxycitrate and (--)-hydroxycitrate combined with medium-chain triglycerides on satiety, fat oxidation, energy expenditure and body weight,” Int J Obes Relat Metab Disord, 25 (7). 1087-1094. 2001.
[20]	S. B. Heymsfield, D. B. Allison, J. R. Vasselli, A. Pietrobelli, D. Greenfield, and C. Nunez, “Garcinia cambogia (hydroxycitric acid) as a potential antiobesity agent: a randomized controlled trial,” JAMA, 280 (18). 1596-1600. 1998.
[21]	L. O. Chuah, W. Y. Ho, B. K. Beh, and S. K. Yeap, “Updates on Antiobesity Effect of Garcinia Origin (-)-HCA,” Evid Based Complement Alternat Med, 2013. 751658. 2013.
[22]	M. Shim, and S. Saab, “Severe hepatotoxicity due to Hydroxycut: a case report,” Dig Dis Sci, 54 (2). 406-408. 2009.
[23]	S. J. Stohs, H. G. Preuss, S. E. Ohia, G. R. Kaats, C. L. Keen, L. D. Williams, and G. A. Burdock, “No evidence demonstrating hepatotoxicity associated with hydroxycitric acid,” World J Gastroenterol, 15 (32). 4087-4089. 2009.
[24]	K. E. Lunsford, A. S. Bodzin, D. C. Reino, H. L. Wang, and R. W. Busuttil, “Dangerous dietary supplements: Garcinia cambogia-associated hepatic failure requiring transplantation,” World J Gastroenterol, 22 (45). 10071-10076. 2016.
[25]	A. Lobb, “Hepatoxicity associated with weight-loss supplements: a case for better post-marketing surveillance,” World J Gastroenterol, 15 (14). 1786-1787. 2009.
[26]	M. Saito, M. Ueno, S. Ogino, K. Kubo, J. Nagata, and M. Takeuchi, “High dose of Garcinia cambogia is effective in suppressing fat accumulation in developing male Zucker obese rats, but highly toxic to the testis,” Food Chem Toxicol, 43 (3). 411-419. 2005.
[27]	P. H. Huang, H. Y. Tsai, C. H. Wang, Y. H. Chen, J. S. Chen, F. Y. Lin, C. P. Lin, T. C. Wu, M. Sata, J. W. Chen, and S. J. Lin, “Moderate intake of red wine improves ischemia-induced neovascularization in diabetic mice--roles of endothelial progenitor cells and nitric oxide,” Atherosclerosis, 212 (2). 426-435. 2010.