In-vitro Prebiotic Activity of Grape Seed Flour Highly Rich in Flavonoid and Dietary Fiber

Ji-Hye Kwon¹, Min Young Kim², Kun-Ho Seo³, Hyeon Gyu Lee^1, and Hyunsook Kim^1,

¹Department of Food and nutrition, Hanyang University, Seoul, South Korea

²Department of Food Science and Technology, Chungbuk National University, Chungju, South Korea

³KU Center for Food Safety, College of Veterinary Medicine, Konkuk University, Seoul, South Korea

Cite this paper:
Ji-Hye Kwon, Min Young Kim, Kun-Ho Seo, Hyeon Gyu Lee and Hyunsook Kim. In-vitro Prebiotic Activity of Grape Seed Flour Highly Rich in Flavonoid and Dietary Fiber. Journal of Food and Nutrition Research. 2018; 6(10):621-625. doi: 10.12691/jfnr-6-10-2

Abstract

Interactions between lactic acid bacteria (LAB) and dietary polyphenols are not fully understood. This study determined the prebiotic effects of grape seed flour (GSF, a byproduct of wine making) and their polyphenol extracts (GSE) on the growth of 10 strains of probiotic LAB recently isolated from the kefir and pathogenic Clostridium perfringens. Growth rate of the LAB, Leuconostoc mesenteroides DH 1608, was the highest among 10 strains of LAB when cultivated with GSF. GSE, containing an equivalent amount of polyphenol in GSF, also promoted the growth of L. mesenteroides DH 1608 but at a lower rate compared to GSF. Growth of C. perfringens decreased significantly at 10 mg/mL of GSF, but GSE did not affect the growth. In conclusion, GSF may be a useful prebiotic dietary supplement due to its selective antimicrobial capacity via stimulation of probiotic bacteria and inhibition of pathogenic bacteria. A symbiotic combination of selected lactic acid bacteria and GSF may improve gut health.

Keywords:
grape seed flour grape seed extracts lactic acid bacteria kefir prebiotics antimicrobial activity

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References:

[1]	Seo, K.-H. et al. (2016). Chardonnay Grape Seed Flour Ameliorates Hepatic Steatosis and Insulin Resistance via Altered Hepatic Gene Expression for Oxidative Stress, Inflammation, and Lipid and Ceramide Synthesis in Diet-Induced Obese Mice. PloS one 11 (12), e0167680.
[2]	Seo, K.H. et al. (2017). Chardonnay grape seed flour supplemented diets alter intestinal microbiota in diet‐induced obese mice. Journal of Food Biochemistry.
[3]	Kim, H. et al. (2014). Dietary supplementation of chardonnay grape seed flour reduces plasma cholesterol concentration, hepatic steatosis, and abdominal fat content in high-fat diet-induced obese hamsters. Journal of agricultural and food chemistry 62 (8), 1919-1925.
[4]	Ozdal, T. et al. (2016). The reciprocal interactions between polyphenols and gut microbiota and effects on bioaccessibility. Nutrients 8 (2), 78.
[5]	Gwiazdowska, D. et al. (2015). The impact of polyphenols on Bifidobacterium growth. Acta Biochim Pol 62 (4), 895-901.
[6]	Hervert-Hernández, D. et al. (2009). Stimulatory role of grape pomace polyphenols on Lactobacillus acidophilus growth. International journal of food microbiology 136 (1), 119-122.
[7]	Parkar, S.G. et al. (2008). The potential influence of fruit polyphenols on colonic microflora and human gut health. International journal of food microbiology 124 (3), 295-298.
[8]	Cueva, C. et al. (2013). In vitro fermentation of grape seed flavan-3-ol fractions by human faecal microbiota: changes in microbial groups and phenolic metabolites. FEMS microbiology ecology 83 (3), 792-805.
[9]	Kim, D.H. et al. (2017) Kefir alleviates obesity and hepatic steatosis in high-fat diet-fed mice by modulation of gut microbiota and mycobiota: targeted and untargeted community analysis with correlation of biomarkers. J Nutr Biochem 44, 35-43.
[10]	Ulusoy, B.H. et al. (2007). An in vitro study on the antibacterial effect of kefir against some food-borne pathogens. Turk Mikrobiyol Cem Derg 37 (2), 103-107.
[11]	Jeong, D. et al. (2017) Modulation of gut microbiota and increase in fecal water content in mice induced by administration of Lactobacillus kefiranofaciens DN1. Food & function 8 (2), 680-686.
[12]	Kim, D.H. et al. (2017). Dual function of Lactobacillus kefiri DH5 in preventing high-fat-diet-induced obesity: direct reduction of cholesterol and upregulation of PPAR-alpha in adipose tissue. Mol Nutr Food Res 61 (11).
[13]	Singleton, V.L. et al. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in enzymology 299, 152-178.
[14]	Lamuela-Raventós, R.M. and Waterhouse, A.L. (1994). A direct HPLC separation of wine phenolics. American Journal of Enology and Viticulture 45 (1), 1-5.
[15]	Tabasco, R. et al. (2011). Effect of grape polyphenols on lactic acid bacteria and bifidobacteria growth: resistance and metabolism. Food Microbiol 28 (7), 1345-52.
[16]	Mehri HadiNezhad, C.D., Nam Fong Han and Farah Hosseinian. (2013). Flaxseed Soluble Dietary Fibre Enhances Lactic Acid Bacterial Survival and Growth in Kefir and Possesses High Antioxidant Capacity. Journal of Food Research 2 (5), 152-163.
[17]	Kottol K., S.P., A. Reni. (2014). Studies on growth effects of catechin on probiotic bacteria. International Journal of Scientific Research 3 (4), 1-3.
[18]	Huber, B. et al. (2003). Influence of polyphenols on bacterial biofilm formation and quorum-sensing. Zeitschrift für Naturforschung C 58 (11-12), 879-884.
[19]	Yoda, Y. et al. (2004). Different susceptibilities of Staphylococcus and Gram-negative rods to epigallocatechin gallate. Journal of Infection and Chemotherapy 10 (1), 55-58.