Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2018, 6(10), 621-625
DOI: 10.12691/jfnr-6-10-2
Open AccessArticle

In-vitro Prebiotic Activity of Grape Seed Flour Highly Rich in Flavonoid and Dietary Fiber

Ji-Hye Kwon1, Min Young Kim2, Kun-Ho Seo3, Hyeon Gyu Lee1, and Hyunsook Kim1,

1Department of Food and nutrition, Hanyang University, Seoul, South Korea

2Department of Food Science and Technology, Chungbuk National University, Chungju, South Korea

3KU Center for Food Safety, College of Veterinary Medicine, Konkuk University, Seoul, South Korea

Pub. Date: October 25, 2018

Cite this paper:
Ji-Hye Kwon, Min Young Kim, Kun-Ho Seo, Hyeon Gyu Lee and Hyunsook Kim. In-vitro Prebiotic Activity of Grape Seed Flour Highly Rich in Flavonoid and Dietary Fiber. Journal of Food and Nutrition Research. 2018; 6(10):621-625. doi: 10.12691/jfnr-6-10-2


Interactions between lactic acid bacteria (LAB) and dietary polyphenols are not fully understood. This study determined the prebiotic effects of grape seed flour (GSF, a byproduct of wine making) and their polyphenol extracts (GSE) on the growth of 10 strains of probiotic LAB recently isolated from the kefir and pathogenic Clostridium perfringens. Growth rate of the LAB, Leuconostoc mesenteroides DH 1608, was the highest among 10 strains of LAB when cultivated with GSF. GSE, containing an equivalent amount of polyphenol in GSF, also promoted the growth of L. mesenteroides DH 1608 but at a lower rate compared to GSF. Growth of C. perfringens decreased significantly at 10 mg/mL of GSF, but GSE did not affect the growth. In conclusion, GSF may be a useful prebiotic dietary supplement due to its selective antimicrobial capacity via stimulation of probiotic bacteria and inhibition of pathogenic bacteria. A symbiotic combination of selected lactic acid bacteria and GSF may improve gut health.

grape seed flour grape seed extracts lactic acid bacteria kefir prebiotics antimicrobial activity

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