The CH₂Cl₂ Extract Fraction from Ficus erecta var. sieboldii (Miq.) King Suppresses Lipopolysaccharide-mediated Inflammatory Responses in Raw264.7 Cells

Young-Min Ham¹, Weon-Jong Yoon¹, Eun Hwa Sohn², Dae Won Park³, Hyelin Jeon^{3, 4}, Yong-Hwan Jung^1, , Sung Ryul Lee^5, and Se Chan Kang^{3, 6,}

¹Biodiversity Research Institute, Jeju Technopark, Seogwipo, Jeju 63608, Republic of Korea

²Department of Herbal Medicine Resource, Kangwon National University, Samcheok 25913, Republic of Korea

³Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea

⁴Genencell Co. Ltd, 120, Heungdeokjungang-ro, Giheung-gu, Yongin-si, Gyeonggi-do, Korea

⁵Department of Integrated Biomedical Science, Cardiovascular and Metabolic Disease Center, College of Medicine, Inje University, Bok Ji-Ro 75, Busanjin-Gu, Busan 47392, Republic of Korea

⁶BioMedical Research Institute, Kyung Hee University, Yongin 17104, Republic of Korea

Cite this paper:
Young-Min Ham, Weon-Jong Yoon, Eun Hwa Sohn, Dae Won Park, Hyelin Jeon, Yong-Hwan Jung, Sung Ryul Lee and Se Chan Kang. The CH₂Cl₂ Extract Fraction from Ficus erecta var. sieboldii (Miq.) King Suppresses Lipopolysaccharide-mediated Inflammatory Responses in Raw264.7 Cells. Journal of Food and Nutrition Research. 2018; 6(6):356-364. doi: 10.12691/jfnr-6-6-2

Abstract

A phytochemical application of leaves from Ficus erecta var. sieboldii (Miq.) King has not been widely investigated. We determined an anti-inflammatory effect of F. erecta extracts on lipopolysaccharide (LPS)-mediated production through modulation of several pro-inflammatory mediators and prostaglandin E2 (PGE₂). Among the F. erecta extracts, the CH₂Cl₂ fraction (CFE) most effectively suppressed the LPS-mediated production of nitric oxide (NO) in Raw264.7 cells. As determined by immunoblotting and PCR, CFE was shown to have an inhibitory effect on LPS-induced mRNA and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). In addition, CFE showed significant inhibitory effects on LPS-mediated production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and PGE₂ (P<0.05), demonstrating its effects on inflammation. The main active compounds that suppressed PGE₂ production were syringaresinol (C1) and 6,7-furano-5-methoxy hydrocoumaric acid (C2). In conclusion, CFE showed an inhibitory effect on LPS-mediated inflammatory responses by suppressing iNOS, COX-2, TNF-α, IL-1β, and IL-6 production. The compounds C1 and C2 showed strong inhibitory effects on LPS-mediated production of PGE₂ and may be applicable as starter compounds for developing anti-inflammatory and anti-nociceptive drugs.

Keywords:
Ficus erecta var. sieboldii (Miq.) King Inflammation Cyclooxygenase-2 Prostaglandin E2 Pro-inflammatory cytokine Syringaresinol 6 7-Furano-5-methoxy Hydrocoumaric acid

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