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Article

The CH2Cl2 Extract Fraction from Ficus erecta var. sieboldii (Miq.) King Suppresses Lipopolysaccharide-mediated Inflammatory Responses in Raw264.7 Cells

1Biodiversity Research Institute, Jeju Technopark, Seogwipo, Jeju 63608, Republic of Korea

2Department of Herbal Medicine Resource, Kangwon National University, Samcheok 25913, Republic of Korea

3Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea

4Genencell Co. Ltd, 120, Heungdeokjungang-ro, Giheung-gu, Yongin-si, Gyeonggi-do, Korea

5Department of Integrated Biomedical Science, Cardiovascular and Metabolic Disease Center, College of Medicine, Inje University, Bok Ji-Ro 75, Busanjin-Gu, Busan 47392, Republic of Korea

6BioMedical Research Institute, Kyung Hee University, Yongin 17104, Republic of Korea


Journal of Food and Nutrition Research. 2018, Vol. 6 No. 6, 356-364
DOI: 10.12691/jfnr-6-6-2
Copyright © 2018 Science and Education Publishing

Cite this paper:
Young-Min Ham, Weon-Jong Yoon, Eun Hwa Sohn, Dae Won Park, Hyelin Jeon, Yong-Hwan Jung, Sung Ryul Lee, Se Chan Kang. The CH2Cl2 Extract Fraction from Ficus erecta var. sieboldii (Miq.) King Suppresses Lipopolysaccharide-mediated Inflammatory Responses in Raw264.7 Cells. Journal of Food and Nutrition Research. 2018; 6(6):356-364. doi: 10.12691/jfnr-6-6-2.

Correspondence to: Se  Chan Kang, Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea. Email: yhjung@jejutp.or.kr; lsr1113@inje.ac.kr; sckang@khu.ac.kr

Abstract

A phytochemical application of leaves from Ficus erecta var. sieboldii (Miq.) King has not been widely investigated. We determined an anti-inflammatory effect of F. erecta extracts on lipopolysaccharide (LPS)-mediated production through modulation of several pro-inflammatory mediators and prostaglandin E2 (PGE2). Among the F. erecta extracts, the CH2Cl2 fraction (CFE) most effectively suppressed the LPS-mediated production of nitric oxide (NO) in Raw264.7 cells. As determined by immunoblotting and PCR, CFE was shown to have an inhibitory effect on LPS-induced mRNA and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). In addition, CFE showed significant inhibitory effects on LPS-mediated production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and PGE2 (P<0.05), demonstrating its effects on inflammation. The main active compounds that suppressed PGE2 production were syringaresinol (C1) and 6,7-furano-5-methoxy hydrocoumaric acid (C2). In conclusion, CFE showed an inhibitory effect on LPS-mediated inflammatory responses by suppressing iNOS, COX-2, TNF-α, IL-1β, and IL-6 production. The compounds C1 and C2 showed strong inhibitory effects on LPS-mediated production of PGE2 and may be applicable as starter compounds for developing anti-inflammatory and anti-nociceptive drugs.

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