Influence of Treatment and Cooking Time on the Antioxidant Capacity of Different Vegetables Used in Atlantic and Mediterranean Diets

Celia García-Rodríguez¹, Mª Ángeles Romero-Rodríguez^1, and Mª Lourdes Vázquez-Odériz^1,

¹Áreas de Nutrición y Bromatología y Tecnología de Alimentos, Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Santiago de Compostela, Campus de Lugo, 27002 Lugo (Spain)

Cite this paper:
Celia García-Rodríguez, Mª Ángeles Romero-Rodríguez and Mª Lourdes Vázquez-Odériz. Influence of Treatment and Cooking Time on the Antioxidant Capacity of Different Vegetables Used in Atlantic and Mediterranean Diets. Journal of Food and Nutrition Research. 2018; 6(4):234-241. doi: 10.12691/jfnr-6-4-5

Abstract

Evidence from epidemiological studies has strongly suggested that diets rich in fruits and vegetables play a vital role in disease prevention. The aim of this study was to determine total phenolic content (TPC) and reducing power (RP) for nine vegetables that are normally consumed in Atlantic and Mediterranean diets. In this study vegetables were analyzed when fresh, and then again after heat processes (cooking in boiling water and steam) were applied for different lengths of time. The vegetable showing highest total phenolic content was the Brussels sprout; while zucchini had the lowest content. Green beans presented the highest reducing power, while peppers and cauliflower presented the lowest values. Heat treatment significantly reduced the concentration of total phenolic content and reducing power in all of tested vegetables; however, steam cooking resulted in lower losses. The loss of the total phenolic content and reducing power were higher when heat processes were applied for longer amounts of time. From a nutritional standpoint, it is advisable to use the least aggressive method (steaming) for as short a time as possible

Keywords:
total phenolic content reducing power Atlantic and Mediterranean diet vegetables

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References:

[1]	Lampe, J.W, “Health effects of vegetables and fruit: Assessing mechanisms of action in human experimental studies,” Am J Clin Nutr, 70, 475S-490S, 1999.
[2]	Carocho, M., Ferreira, I.C.F.R, “A review on antioxidant, prooxidants and related controversy: natural and synthetic compounds, screening and analysis methodologies and future perspectives,” Food Chem Toxicol, 51, 15-25, 2013.
[3]	Ness, A.R., Powles, J.W, “Fruit and Vegetables, and Cardiovascular Disease: A Review,” Int J Epidemiol, 26, 1-13, 1997.
[4]	Block, G., Patterson, B., Subar, A, “Fruit, vegetables, and cancer prevention: A review of the epidemiological evidence,” Nutr Cancer, 18, 1-29, 1992.
[5]	Giovannini, C., Masella, R, “Role of polyphenols in cell death control”, Nutr Neurosci, 15, 134-149, 2012.
[6]	Kongkachuichai, R., Charoensiri, R., Yakoh, K., Kringkasemsee, A., Insung, P, “Nutrients value and antioxidant content of indigenous vegetables from Southern Thailand,” Food Chem, 173, 838–846, 2015.
[7]	Quiñones, M., Miguel, M., Aleixandre, A, “The Polyphenols, naturally occurring compounds with beneficial effects on cardiovascular disease,” Nutr Hosp, 27, 76-89, 2012.
[8]	Agarwal, A., Allamaneni, S.S.R, “Oxidants and antioxidants in human fertility,” Middle East Fertil Soc J, 9, 187-197, 2004.
[9]	Moo-Huchin, V.M., Moo-Huchin, M.I., Estrada-León, R.J., Cuevas-Glory, L., Estrada-Mota, I.A., Ortiz-Vázquez, E., Betancur-Ancona, D., Sauri-Duch, E, “Antioxidant compounds, antioxidant activity and phenolic content in peel from three tropical fruits from Yucatan, Mexico,” Food Chem, 166, 17-22, 2015.
[10]	Kaulmann, A., Jonvillem, M.C., Schneider, Y.J., Hoffmann, L., Bohn, T, “Carotenoids, polyphenols and micronutrient profiles of Brassica oleraceae and plum varieties and their contribution to measures of total antioxidant capacity,” Food Chem, 155, 240-250, 2014.
[11]	Kuskoski, E.M., Asuero, A.G., Troncoso, A.M., Mancini-Filho, J., Fett, R, “Aplicação de diversos métodos químicos para determinar atividade antioxidante em polpa de frutas,” Ciência e Tecnologia de Alimentos, 25, 726-732, 2005.
[12]	Chemah, T.C., Aminah, A., Noriham, A., Wan Aida, W.M, “Determination of pitaya seeds as a natural antioxidant and source of essential fatty acids,” Int Food Res J, 17, 1003-1010, 2010.
[13]	Manach, C., Donovan, J.L, “Pharmacokinetics and metabolism of dietary flavonoids in humans,” Free Radical Res, 38, 771-785, 2004.
[14]	Cai, W., Gu, X., Tang, J, “Extraction, purification and characterization of the flavonoids from Opuntia milpa alta skin,” Czech J Food Sci, 28, 108-116, 2010.
[15]	Kabir, F., Tow, W.W., Hamauzum Y., Katayama, S., Tanaka, S., Nakamura, S, “Antioxidant and cytoprotective activities of extracts prepared from fruit and vegetable wastes and by-products,” Food Chem, 167, 358-362, 2015.
[16]	Francisco, M., Velasco, P., Romero, A., Vázquez, L., Cartea, M.E, “Sensory quality of turnip greens and turnip tops grown in northwestern Spain,” Eur Food Res Technol, 230, 281–290, 2009.
[17]	Singleton, V. L., Orthofer, R., Lamuela-Raventós, R. M, “Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagen,” Methods Enzymol, 299, 152-178, 1999.
[18]	Oboh, G, “Effect of blanching on the antioxidant properties of some tropical green leafy vegetables”, Food Sci Technol, 38, 513-517, 2005.
[19]	Atmani, D., Chaher, N., Berboucha, M., Ayouni, K., Lounis, H., Boudaoud, H., Debbache, N., Atmani, D, “Antioxidant capacity and phenol content of selected Algerian medicinal plants,” Food Chem, 112, 303-309, 2009.
[20]	Stajčić, S., Ćetković, G., Čanadanović-Brunet, J., Djilas, S., Mandić, A., Četojević-Simin, D, “Tomato waste: Carotenoids content, antioxidant and cell growth activities,” Food Chem, 172, 225-232, 2015.
[21]	Sreeramulu, D., Raghunath, M, “Antioxidant activity and phenolic content of roots, tubers and vegetables commonly consumed in India,” Food Res Int, 43, 1017-1020, 2010.
[22]	Tharasena, B., Lawan, S, “Phenolics, Flavonoids and Antioxidant Activity of Vegetables as Thai Side Dish,” APCBEE Procedia, 8, 99-104, 2014.
[23]	Korekar, G., Dolkar, P., Singh, H., Srivastava, R.B., Stobdan, T, “Variability and the genotypic effect on antioxidant activity, total phenolics, carotenoids and ascorbic acid content in seventeen natural population of Seabuckthorn (Hippophae rhamnoides L.) from trans-Himalaya,” Food Sci Technol, 55, 157-162, 2014.
[24]	Li, H., Deng, Z., Liu, R., Zhu, H., Draves, J., Marcone, M., Sun, Y., Tsao, R, “Characterization of phenolics, betacyanins and antioxidant activities of the seed, leaf, sprout, flower and stalk extracts of three Amaranthus species,” J Food Compost Anal, 37, 75-81, 2015.
[25]	Ismail, A., Marjan, Z. M., Foong, C. W, “Total antioxidant activity and phenolic content in selected vegetables,” Food Chem, 87, 581-586, 2004.
[26]	Cieslik, E., Greda, A., Adamus, W, “Contents of polyphenols in fruit and vegetables,” Food Chem, 94, 135-142, 2006.
[27]	Hervert-Hernández, D., García, O. P., Rosado, J. L., Goñi, I, “The contribution of fruits and vegetables to dietary intake of polyphenols and antioxidant capacity in a Mexican rural diet: Importance of fruit and vegetable variety,” Food Res Int, 44, 1182-1189, 2011.
[28]	Sumczynski, D., Bubelova, Z., Sneyd, J., Erb-Weber, S., Mlcek, J, “Total phenolics, flavonoids, antioxidant activity, crude fibre and digestibility in non-traditional wheat flakes and muesli,” Food Chem, 74, 319-325, 2015.
[29]	Zhang, D., Hamauzu, Y, “Phenolics, ascorbic acid, carotenoids and antioxidant activity of broccoli and their changes during conventional and microwave cooking”. Food Chem, 88, 503-509. 2004.
[30]	Wachtel-Galor, S., Wong, K. W., Benzie, I.F.F, “The effect of cooking on Brassica vegetables,” Food Chem, 110, 706-710, 2008.
[31]	Gorinstein, S., Jastrzebski, Z., Leontowicz, H., Leontowicz, M., Namiesnik, J., Najman, K., Park, Y. S., Heo, B.G., Cho, J.Y., Bae, J.H, “Comparative control of the bioactivity of some frequently consumed vegetables subjected to different processing conditions,” Food Control, 20, 407-413, 2009.
[32]	Faller, A. L.K., Fialho, E, “The antioxidant capacity and polyphenol content of organic and conventional retail vegetables after domestic cooking,” Food Res Int, 42, 210-215, 2009.
[33]	Miglio, C., Chiavaro, E., Visconti, A., Fogliano, V., Pellegrini, N, “Effects of Different Cooking Methods on Nutritional and Physicochemical Characteristics of Selected Vegetables,” J Agric Food Chem, 56, 139-147, 2008.
[34]	Mazzeo, T., N’Dri, D., Chiavaro, E., Visconti, A., Fogliano, V., Pellegrini, N, “Effect of two cooking procedures on phytochemical compounds, total antioxidant capacity and colour of selected frozen vegetables,” Food Chem, 128, 627-633, 2011.
[35]	Shyamala, B. N., Gupta, S., Lakshmi, A. J., Prakash, T. J, “Leafy vegetable extracts—antioxidant activity and effect on storage stability of heated oils,” Innov Food Sci Emerg, 6, 239-245, 2005.
[36]	Andarwulan, N., Batari, R., Sandrasari, D. A., Bolling, B., Wijaya, H, “Flavonoid content and antioxidant activity of vegetables from Indonesia,” Food Chem, 121, 1231-1235, 2010.
[37]	Özen, T, “Antioxidant activity of wild edible plants in the Black Sea Region of Turkey,” Int J Fats Oils, 61, 86-94, 2010.
[38]	Kim, S. J., Cho, A. R., Han, J, “Antioxidant and antimicrobial activities of leafy green vegetable extracts and their applications to meat product preservation,” Food Control, 29, 112-120, 2013.