Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: https://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2016, 4(10), 646-652
DOI: 10.12691/jfnr-4-10-3
Open AccessArticle

Application of Pulsed Electric Field for Microorganisms Inactivation in Date palm Fruits

M. E. A. Mohammed1, 2, , A. H. Amer Eissa2 and S. M. Aleid3

1Date Palm Research Center of excellence, King Faisal University, KSA

2Agricultural Engineering Department, Faculty Agriculture, Minoufiya University, Egypt

3Food Science and Nutrition Department, Agricultural and Food Sciences College, King Faisal University, KSA

Pub. Date: September 18, 2016

Cite this paper:
M. E. A. Mohammed, A. H. Amer Eissa and S. M. Aleid. Application of Pulsed Electric Field for Microorganisms Inactivation in Date palm Fruits. Journal of Food and Nutrition Research. 2016; 4(10):646-652. doi: 10.12691/jfnr-4-10-3

Abstract

Pulsed electric field (PEF) processing of liquid and semisolid food is a nonthermal technology alternative to traditional thermal preservation method with preserving nutritional and sensory values of food. In this study, the components of a laboratory scale prototype of PEF system were designed and constructed. Evaluating the performance of the prototype was carried out to inactivate the existing microorganisms (mesophilic aerobic bacteria, Yeasts and Molds) in semi-solid pitted date palm fruits, which was defined in terms of applied intensity of electric field and pulses number at a constant duration of pulse (40 µs) and (1 Hz) pulse frequency. The microbial count was decreased with the increase in intensity of electric field and pulses number. The electric field intensity of 10.82 kV/cm and 120 pulses lead to reduced total microbial counts of 1.18×104 cfu/g to less than 10 cfu/g of mesophilic aerobic bacteria in most treated samples. The electric field intensity of 8.84 kV/cm and 90 pulses lead to reduce total microbial counts of 3.27×103 cfu/g of yeasts and molds to less than 10 cfu/g that meet Saudi standards requirements. Non-detectable levels of yeasts and molds in most treated samples were observed when 10.82 kV/cm of electric field intensity and 60 of pulses number were applied. The current results indicated that PEF technology is promising as a non-thermal method for inactivation of microorganisms on date palm fruits processing.

Keywords:
pulsed electric field date palm fruits microbial contamination preservation non-thermal technology

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References:

[1]  FAO. 2012. FAOSTAT statistical database (available at faostat.fao.org).
 
[2]  Hamad, S. H. and Aleid, S. M., “Use of electrolyzed oxidizing water for the control of microbial contamination of date fruits”, International Journal of Microbiology and Immunology Research. 2(3), 29-36, 2013.
 
[3]  Benkeblia, N., “Storage technologies for date palm fruits (phoenix dactyliferous current and potential techniques”, ACTA hort, (ISHS) 994, 151-154, 2013.
 
[4]  Lado, B. H., Yousef, A. E., “Selection and identification of a Listeria monocytogenes target strain for pulsed electric field process optimization”, Appl. Environ. Microbiol. 69, 2223-2229, 2003.
 
[5]  Zimmermann, U., Electrical breakdown, “electropermeabilization and electrofusion”, Reviews of Physiology, Biochemistry and Pharmacology, 105, 175-256, 1986.
 
[6]  Toepfl, S., Mathys, A., Heinz, V., Knorr, D., “Potential of high hydrostatic pressure and pulsed electric fields for energy efficient and environmentally friendly food processing” Food Reviews International, 22(4), 405-423, 2006.
 
[7]  Mohamed, M. E. A., Amer Eissa, H. A., Pulsed Electric Fields for Food Processing Technology, Structure and Function of Food Engineering, Amer Eissa A. H. A. Edited. InTech, 2012, Chapter 11, 275-306.
 
[8]  Zimmermann, U., Benz, R., “Dependence of the electrical breakdown voltage on the charging time in Valonia utricularis”, L Membr. Bioi., 53, 33-43, 1980.
 
[9]  Zhang, Q. H., Barbosa-Canovas, G. V., Swanson, B. G., “Engineering aspects of pulsed electric fields pasteurization”, Journal of Food Engineering, 25, 261-281, 1995.
 
[10]  Ho, S.Y., Mittal, G. S., Cross, J. D., Griffiths, M. W., “Inactivation of Pseudomonas fluoresces by high voltage electric pulses”. J. Food Sci. 60, 1337-1340, 1995.
 
[11]  Weise, H. G. G., Loeffler, M. J., “Over view on pulsed power applications”, Proceedings of the International Conference on Pulsed Power Applications, Gelsenkirchen, Germany, 2001.
 
[12]  Floury, J., Grosset, N., Leconte, N., Pasco, M., Madec, M., Jeantet, R., “Continuous raw skim milk processing by pulsed electric field at non-lethal temperature: Effect on microbial inactivation and functional properties”, Lait. 86, 43-57, 2005.
 
[13]  Barbosa-Cánovas, G. V., Gongora-Nieto, M. M., Pothakamury, U. R., Swanson, B. G., Fundamentals of high-intensity pulsed electric fields (PEF), In Preservation of foods with pulsed electric fields, G. V. Barbosa-Cánovas et al. edited. Academic Press. San Diego, CA, USA, 1999, 1-155.
 
[14]  Dutreux, N., Notermans, S., Go´ngora-Nieto, M. M., Barbosa-Ca´novas, G. V. Swanson, B. G., “Effects of combine exposure of micrococcus lutes to nisin and pulsed electric fields”. J. Food Microbial. 60, 147-152, 2000.
 
[15]  Sampedro, F., A. Rivas, D., Rodrigo, A., Martıne, Z., Rodrigo, M., “Pulsed electric fields inactivation of Lactobacillus plant arum in an orange juice–milk based beverage”, Journal of Food Engineering, 80, 931-938, 2007.
 
[16]  Peleg, M., A “model of microbial survival after exposure to pulsed electric fields”, Journal of the Food Science & Agriculture, 67, 93-99, 1995.
 
[17]  Qin, B. L., Barbosa-Cánovas, G. V., Swanson, B., Pedrow, P. D., Olsen, R. G., “Inactivating microorganisms using a pulsed electric field continuous treatments system”, IEEE Trans. Ind. Appl. 34, 43-50, 1998.
 
[18]  Raso, J., Alvarez, I., Condün, S., Trepat, F. J. S., “Predicting inactivation of Salmonella senftenberg by pulsed electric fields”, Innov. Food Sci. Emerg. Technol., 1, 21-29, 2000.
 
[19]  Unal, R., Yousef, A. E., Dunne, P., “Spectrofluorimetric assessment of bacterial cell membrane damage by pulsed electric field” Food Sci. Emerg. Technol., 3, 247-254, 2002.
 
[20]  Heinz, V., Alvarez I., Angersbach, A., Knorr, D., “Preservation of liquid foods by high intensity electric fields basic concepts for process design”, Trends in Food Science and Technology. 12, 103-111, 2002.
 
[21]  Malicki, A., Oziemblowski, M., Molenda, J., Trziszka, T., Bruzewicz, S., “Effect of Pulsed Electric Field (PEF) on Escherichia coli within the liquid whole egg”, Bull. Vet. Inst. Pulawy, 48, 371-373, 2004.
 
[22]  Aleid, S. M., Hassan, B. H., Almaiman, S. A., Al-Kahtanim, S H., Ismail, S. M. “Microbial loads and physicochemical characteristics of fruits from four Saudi date palm tree cultivars: conformity with applicable date standards”, Food and Nutrition Sciences, 5, 316-327, 2014.
 
[23]  Bartels, P., “Definition and guidelines for reporting on pulsed electric field treatment”, Workshop NPD Berlin, Germany, 2001.
 
[24]  Castro, A. J., Barbosa-Cánovas, G. V., Swanson, B. G., “Microbial inactivation of foods by pulsed electric fields”, J. Food Process Preserve. 17, 47-73, 1993.
 
[25]  Wouters, P.C, Bos, A. P., Ueckert, L., “Membrane permeabilization in relation to inactivation kinetics of Lactobacillus species due to pulsed electric fields”, Appl. Environ. Microbial, 67, 3092-3101, 2001.
 
[26]  Jayaram, S., Castle, G. S. P., Margaritis, A., “The effect of high field DC pulse and liquid medium conductivity on survivability of Lactobacillusbrevis”, Biotechnol. Bioeng. 40,117-122, 1993.
 
[27]  Qin, B. L., Chang, F., Barbosa-Cfinovas, G. V., Swanson, B. G., “Nonthermal inactivation of Saccharomyces cerevisiae in apple juice using pulsed electric fields”, Lebensm .Wiss. Technol. 28, 564-568, 1995.
 
[28]  Jeyamkondan, S., Jayas, D. S., Holley, R. A., “Pulsed electric field processing of foods”, J Food Prot. 62 (9), 1088-1096, 1999.
 
[29]  Yeom, H.W., Streaker, C. B., Zhang, Q. H., Min, D. B., Effects of pulsed electric fields on the activities of microorganisms and pectin methyl esterase in orange juice, Journal of food science, 65 (8), 1359-1363, 2000.