Low Cytotoxic D-mannitol Isolated from the Industrial Wastewater of Agaricus bisporus

Jinmei Lin^{1, 2}, Jiafu Huang¹, Junyu Chen³, Zhichao Lin¹, Yixin Ou¹, Liyun Yao¹, Xiufen Zhang¹, Qianjin Kang⁴ and Yutian Pan^1,

¹Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, China

²College of Chemistry and Environment, Minnan Normal University, Zhangzhou, China

³Dongshan Entry-Exit Inspection and Quarantine Bureau, Zhangzhou, China

⁴Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai, China

Cite this paper:
Jinmei Lin, Jiafu Huang, Junyu Chen, Zhichao Lin, Yixin Ou, Liyun Yao, Xiufen Zhang, Qianjin Kang and Yutian Pan. Low Cytotoxic D-mannitol Isolated from the Industrial Wastewater of Agaricus bisporus. Journal of Food and Nutrition Research. 2016; 4(9):610-614. doi: 10.12691/jfnr-4-9-8

Abstract

Agaricus bisporus is an edible mushroom, and mainly made in canned food for storage and trading. However, large amount of industrial wastewater containing various components is generated during the production of canned mushroom. This study describes the method for purification of D-mannitol and evaluation of its bioactivity from the industrial wastewater of A. bisporus. Its structure was confirmed by GC, IR, UV, HPLC, HPLC-MS, DSC analysis. The content of D-mannitol in the concentrated industrial wastewater (≥45°Be') of A. bisporus was determined to more than 32.0%. Comparing with the D-mannitol standard, this purified MN6 had a higher purity and lower cytotoxicity to L929 fibroblast evaluated by MTS assay. These results revealed that the A. bisporus industrial wastewater is a good resource for obtanning natural D-mannitol with high quality.

Keywords:
D-mannitol Agaricus bisporus wastewater lower cytotoxicit

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