In Vitro and in Vivo Evaluation of Antidiabetic Activity of Leaf Essential Oil of Pulicaria inuloides-Asteraceae

Nabil Qaid M. Al-Hajj^{1, 2}, Methaq algabr³, Hafiz Rizwan Sharif¹, Waleed Aboshora¹ and Hongxin Wang^1,

¹State key laboratory of Food science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, P.R. China

²Marine Science and Biological Research Authority, Aden, P.O. Box-1231, Aden, Yemen

³Department of chemistry, Faculty of Applied Science Hajjah University, P.O. Box 80004, Yemen

Cite this paper:
Nabil Qaid M. Al-Hajj, Methaq algabr, Hafiz Rizwan Sharif, Waleed Aboshora and Hongxin Wang. In Vitro and in Vivo Evaluation of Antidiabetic Activity of Leaf Essential Oil of Pulicaria inuloides-Asteraceae. Journal of Food and Nutrition Research. 2016; 4(7):461-470. doi: 10.12691/jfnr-4-7-8

Abstract

The essential oil of leaf of P. inuloides was extracted by using hydrodistillation process and the volatile components investigated with the help of gas chromatography coupled with mass spectrometry. Diabetic rats were treated with a 400 mg/kg dose of essential oil of P. inuloides orally for 3 h (acute) or 21 days (chronic). In acute model blood glucose levels were monitored at specific intervals. In the chronic model blood samples were collected from overnight fasted diabetic rats on day 21 to estimate blood glucose level. And the body weight, serum lipid profile and activities of liver and kidney enzymes were measured. Histopathological observations of liver sections were also studied. Total seventy compounds were identified as 2-Cyclohexen-1-one, 2-methyl-5-(1-methylethyl)-, (S)- 58.19%; Oxirane, 2-ethyl-2-methyl- 8.25%; 1,2-Benzenedicarboxylic acid, diisooctyl ester 13.18%; n-Hexadecanoic acid 2.12% by gas chromatography coupled with mass spectrometry. Treatment of diabetic rats with 400 mg/kg of P. inuloides proved to have significant (P < 0.05) antihyperglycemic effect and have the capacity to correct the metabolic disturbances associated with diabetes. Histopathological studies showed that the essential oil of P. inuloides reinforced the healing of liver.

Keywords:
diabetes rats essential oil Pulicaria inuloides Histopathology

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]	Luo Q, Cai Y, Yan J, Sun M, Corke H. Hypoglycemic and hypolipidemic effects and antioxidant activity of fruit extracts from Lycium barbarum. Life sciences 2004; 76(2): 137-149.
[2]	Ruta LM, Magliano DJ, LeMesurier R, Taylor HR, Zimmet PZ, Shaw JE. Prevalence of diabetic retinopathy in Type 2 diabetes in developing and developed countries. Diabetic medicine 2013; 30 (4): 387-398.
[3]	Hu FB. Globalization of Diabetes The role of diet, lifestyle, and genes. Diabetes care 2011; 34(6): 1249-1257.
[4]	Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes estimates for the year 2000 and projections for 2030. Diabetes care 2004; 27(5): 1047-1053.
[5]	Chattopadhya RR. A comparative evaluation of some blood sugar lowering agents of plant origin. Journal of ethnopharmacology 1999; 67(3): 367-372.
[6]	Hanbali FE, Akssira M, Ezoubeiri A, Mellouki F, Benherraf A, Blazquez AM, Boira H. Chemical composition and antibacterial activity of essential oil of Pulicaria odora L. Journal of ethnopharmacology 2005; 99(3): 399-401.
[7]	Dubaie AS, El-Khulaidi AA. Medicinal and aromatic plants in Yemen, deployment-components of effective-uses. Ebadi Center for studies and Publishing Sana’a-Yemen 2005; 127.
[8]	Ralchenko, YU, Kramida AE, Reader J. NIST atomic spectra database (version 4.0). National Institute of Standards and Technology, Gaithersburg, MD, 2010.
[9]	McCue PP, Shetty K. Inhibitory effects of rosmarinic acid extracts on porcine pancreatic amylase in vitro. Asia Pacific Journal of Clinical Nutrition 2004; 13(1): 101-106.
[10]	Kim YM, Jeong YK, Wang MH, Lee WY, Rhee HI. Inhibitory effect of pine extract on α-glucosidase activity and postprandial hyperglycemia. Nutrition 2005; 21(6): 756-761.
[11]	Arora MK, Reddy K, Balakumar P. The low dose combination of fenofibrate and rosiglitazone halts the progression of diabetes-induced experimental nephropathy. European journal of pharmacology 2010; 636(1): 137-144.
[12]	Rothan HA, Zulqarnain M, Ammar YA, Tan EC, Rahman NA,Yusof R. Screening of antiviral activities in medicinal plants extracts against dengue virus using dengue NS2B-NS3 protease assay.Trop Biomed2014;31: 286-296.
[13]	Cannas S, Molicotti P, Ruggeri M, Cubeddu M, Sanguinetti M, Marongiu B, et al. Antimycotic activity ofMyrtus communis L. towards Candidaspp. from isolates.J Infect Dev Countr2013;7: 295-298.
[14]	Sukphan P, Sritularak B, Mekboonsonglarp W, Lipipun V, Likhitwitayawuid K. Chemical constituents of Dendrobium venustumand their antimalarial and anti-herpetic properties.Nat Prod Commun2014;9: 825-827.
[15]	Doughari JH, Ndakidemi PA, Human IS, Benade S. Antioxidant, antimicrobial and antiverotoxic potentials of extracts ofCurtisia dentata. J Ethnopharmacol2012;141: 1041-1050.
[16]	Inouyea S, Takizawab T, Yamaguchia H. Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact.J Antimicrob Chemother2001;47: 565-573.
[17]	Chiang HM, Chiu HH, Lai YM, Chen CY, Chiang HL. Carbonyl species characteristics during the evaporation of essential oils. Atmos Environ2010;44: 2240-2247.
[18]	Brownlee M, Cerami A. The biochemistry of the complications of diabetes mellitus. Annual review of biochemistry 1981; 50 (1): 385-432.
[19]	Chehade JM, Mooradian AD. A rational approach to drug therapy of type 2 diabetes mellitus. Drugs 2000; 60 (1): 95-113.
[20]	Charpentier G. (2002). Oral combination therapy for type 2 diabetes. Diabetes/metabolism research and reviews 2002; 18 (S3): S70-S76.
[21]	Peters AL, Davidson M B, Schriger D L, Hasselblad V. A clinical approach for the diagnosis of diabetes mellitus: an analysis using glycosylated hemoglobin levels. Jama 1996; 276(15): 1246-1252.
[22]	Pupim LB, Heimbürger O, Qureshi AR, TALP IKIZLER, Stenvinkel P. Accelerated lean body mass loss in incident chronic dialysis patients with diabetes mellitus. Kidney international 2005; 68(5): 2368-2374.
[23]	Pareek H, Sharma S, Khajja BS, Jain K., Jain GC. Evaluation of hypoglycemic and anti-hyperglycemic potential of Tridax procumbens (Linn.).BMC complementary and alternative medicine 2009; 9(1): 48.
[24]	Subramanian R, Asmawi MZ, Sadikun A. In vitro alpha-glucosidase and alpha-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide. Acta Biochim Pol 2008; 55(2): 391-398.
[25]	‏Chaurasia A, Kharya MD, Sharma B, Roy P. Glucose metabolism and diabetogenic gene expression analysis of chloroform fraction of Andrographis paniculata (Nees) whole herb in diabetic albino mice. Journal of Complementary and Integrative Medicine 2012; 9 (1).‏
[26]	Valdiani A, Kadir MA, Tan SG, Talei D, Abdullah MP, Nikza, S. Nain-e Havandi Andrographis paniculata present yesterday, absent today: a plenary review on underutilized herb of Iran’s pharmaceutical plants. Molecular biology reports 2012; 39(5): 5409-5424.‏
[27]	Nugroho AE., Andrie M, Warditiani NK, Siswanto E, Pramono S, Lukitaningsih E. Antidiabetic and antihiperlipidemic effect of Andrographis paniculata (Burm. f.) Nees and andrographolide in high-fructose-fat-fed rats. Indian journal of pharmacology 2012; 44(3): 377.‏
[28]	Singh D, Nigam R, Pandey V, Singh P. Status of lipid peroxidation and antioxidant enzymes in stress induced rats when treated with Andrographis paniculata extract and vitamin C. Vet Practb2012; 13(1): 60-62.‏
[29]	Rajesh P, Sathish S, Srinivasa C, Selvaraj J, Balasubramanian K. Diethyl hexyl phthalate (DEHP) is associated with insulin resistance in adipose tissue of male rat: protective role of antioxidant vitamins (C & E).Journal of cellular biochemistry 2013; 114(3): 558-569.‏
[30]	Daisy P, Rayan. A, Rajathi D. Hypoglycemic and other related effects of Elephantopus scaber extracts on alloxan induced diabetic rats. J. Biol. Sci 2007; 7(2): 433-437.
[31]	Luka C, Tijjani, H. Comparative studies of the aqueous extracts of Ocimum gratissimum, aloe vera, brassica oleraceaand ipomoea batatas on some biochemical parameters in diabetic rats. IOSR Journal of Pharmacy and Biological Sciences 2013; 6 (3): 23-29.
[32]	Singh NS, Geetha M, Amudha P, Chakraborty A. Evaluation of anti-diabetic activity of methanol extract of Flacourtia jangomas (Lour) in streptozotocin induced diabetic rats. International Journal of Pharma and Bio Sciences 2010; 1(3).
[33]	Ragbetli C. Ceylan E. Effect of Streptozotocin on Biochemical Parameters in Rats. Asian Journal of Chemistry 2010; 22(3): 2375.
[34]	El-Demerdash FM., Yousef MI, El-Naga NA. Biochemical study on the hypoglycemic effects of onion and garlic in alloxan-induced diabetic rats. Food and Chemical Toxicology 2005; 43(1): 57-63.