Polyphenol Extracted from Ecklonia cava Increases Insulin-mediated Glucose Uptake in 3T3-L1 Cells and Reduces Fasting Blood Glucose Levels in C57BL/KsJ db/db Mice

Yeon-Joo Lee¹, Kye-Yoon Yoon¹, Ok-Hwan Lee², Kui-Jin Kim^1, and Boo-Yong Lee^1,

¹Department of Food Science and Biotechnology, College of Life Science, CHA University, Seongnam, Kyonggi, South Korea

²Department of Food Science and Biotechnology, Kangwon National University, Chuncheon, South, Korea

Cite this paper:
Yeon-Joo Lee, Kye-Yoon Yoon, Ok-Hwan Lee, Kui-Jin Kim and Boo-Yong Lee. Polyphenol Extracted from Ecklonia cava Increases Insulin-mediated Glucose Uptake in 3T3-L1 Cells and Reduces Fasting Blood Glucose Levels in C57BL/KsJ db/db Mice. Journal of Food and Nutrition Research. 2016; 4(2):126-130. doi: 10.12691/jfnr-4-2-10

Abstract

Previous study suggested that polyphenol-enriched extracts from Ecklonia cava (PREC, Seapolynol) have an inhibitory effect on lipid accumulation in vitro and in vivo. Based on these results, we evaluated the effect of PREC on insulin-mediated glucose uptake in 3T3-L1 cells and in male diabetic C57BL/KsJ-db/db mice. The mice were divided into four groups, including db/db, Rosiglitazone 10mg/kg/day, PREC 60mg/kg/day and PREC 150mg/kg/day. Treatment with PREC upregulated glucose uptake-associated gene expression and improved glucose uptake in fully differentiated 3T3-L1 adipocytes. Additionally, α-glucosidase (α-carbohydrate-hydrolase) was inhibited by PREC in a dose-dependent manner. Moreover, PREC effectively improved GLUT4-associated gene expression and suppressed fasting blood glucose levels. These results suggest that PREC may have a mitigating effect on hyperglycaemia and could help to improve blood glucose levels in diabetes patients.

Keywords:
3T3-L1 Seapolynol diabetes insulin sensitivity

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