Influence of Feeding System on Lipids and Proteins Oxidation, and Antioxidant Enzymes Activities of Meat from Aberdeen Angus Steers

A. Terevinto¹, M.C. Cabrera^{1, 2} and A. Saadoun^2,

¹Dpto Producción Animal & Pasturas, Laboratorio Calidad de Alimentos, Facultad de Agronomía, Universidad de la República. Av. Garzón 809. Montevideo. Uruguay.

²Fisiología & Nutrición, Facultad de Ciencias, Universidad de la República. Calle Igua 4225. Montevideo. Uruguay

Cite this paper:
A. Terevinto, M.C. Cabrera and A. Saadoun. Influence of Feeding System on Lipids and Proteins Oxidation, and Antioxidant Enzymes Activities of Meat from Aberdeen Angus Steers. Journal of Food and Nutrition Research. 2015; 3(9):581-586. doi: 10.12691/jfnr-3-9-4

Abstract

Three feeding system were investigated to determine if any of them is more suitable to ensure a better antioxidant protection to meat. Animals were produced on pasture, pasture supplemented with corn grain, or feedlot. TBARS, protein carbonyls and antioxidant enzyme activities have been determined in fresh ad aged meat from Biceps femoris of Aberdeen Angus steers. No feeding system showed clear protection against lipids and protein oxidation in fresh meat. However, aged meat suffer always lipids and protein oxidation independently of the feeding system. There is not a clear pattern of the action of the catalase antioxidant enzyme (no significant main effect). However, pasture based feeding system present a lower SOD (0.96 UI) and GPx (9.22 nmoles/min/mg protein) activity, versus feedlot (1.12 UI and 11.48 nmoles/min/mg protein, respectively). Based on the results of the present investigation, it seems difficult to conclude about the best feeding system advised to minimize the lipids and protein oxidation of meat.

Keywords:
FAberdeen angus meat TBARS protein carbonyls catalase SOD GPx pasture feedlot

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References:

[1]	Ouali, A, Herrera-Mendez, C. H, Coulis, G, Becila, S, Boudjellal, A, Aubry, L, and Sentandreu, M. A, Revisiting the conversion of muscle into meat and the underlying mechanisms, Meat Science, 2006, 74, 44-58.
[2]	Falowo, A. B, Fayemi, P.O, Muchenje, V, Natural antioxidants against lipid–protein oxidative deterioration in meat and meat : A review, Food Research International, 2014, 64, 171-181.
[3]	Insani, E. M, Eyherabide, A, Grigioni, G, Sancho, A. M, Pensel, N. A, and Descalzo, A. M, Oxidative stability and its relationship with natural antioxidants during refrigerated display of beef produced in Argentina, Meat Science, 2008, 79, 444-452.
[4]	Descalzo, A. M, Insani, E. M, Biolatto, A, Sancho, A. M, García, P. T, Pensel, N. A, and Josifovich, J. A, Influence of pasture or grain-based diets supplemented with vitamin E on antioxidant/oxidative balance of Argentine beef, Meat Science, 2005, 70: 35-44.
[5]	Mercier, Y, Gatellier, P, and Renerre, M, Lipid and protein oxidation in vitro, and antioxidant potential in meat from Charolais cows finished on pasture or mixed diet, Meat Science, 2004, 66, 467-473.
[6]	Gatellier, P, Mercier, Y, and Renerre, M, Effect of diet finishing mode (pasture or mixed diet) on antioxidant status of Charolais bovine meat, Meat Science, 2004, 67: 385-394.
[7]	Lynch, S. M, and Frei, B, Mechanisms of copper- and iron-dependent oxidative modification of human low-density lipoprotein, Journal of Lipid Research, 1993, 34, 1745-1751.
[8]	Stoscheck, C. M, Quantitation of Protein, Method of Enzymology, 1990, 182, 50-68.
[9]	Aebi, H, Catalase in vitro. In L. Packer (Ed), Oxygen radicals in biological systems, Method of Enzymology, 1984, 105, 121-126.
[10]	Marklund, S, and Marklund, G, Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase, European Journal of Biochemistry, 1974, 47, 469-474.
[11]	DeVore, V. R. and Greene, B. E, Glutathione peroxidase in post-rigor bovine semitendinosus muscle, Journal of Food Science, 1982, 47, 1406-1409.
[12]	Günzler, A, and Flohé, L, Glutathione peroxidase. In R. A. Greenwald (Ed), CRC Handbook of methods for oxygen radical research, 1985, 1, 285-290.
[13]	Gatellier, P, Mercier, Y, Juin, H, and Renerre, M, Effect of finishing mode (pasture- or mixed diet) on lipid composition, colour stability and lipid oxidation in meat from Charolais cattle, Meat Science, 2005, 69, 175-186.
[14]	Pouzo, L.B, Descalzo, A.M, Zaritzkya, N.E, Rossetti, N, Pavan, E, Antioxidant status, lipid and color stability of aged beef from grazing steers supplemented with corn grain and increasing levels of flaxseed, Meat Science, 2016,111, 1-8.
[15]	Descalzo, A.M, Rossetti, L, Grigioni, G, Irurueta, M, Sancho, A.M, Carrete, J, and Pensel, N.A, Antioxidant status and odour profile in fresh beef from pasture or grain-fed cattle, Meat Science, 2007, 75, 309-317.
[16]	Renerre, M, Dumont, F, and Gatellier, P, Antioxidant enzyme activities in beef in relation to oxidation of lipid and myoglobin, Meat Science, 1996, 43, 111-121.
[17]	Ramos, A, Cabrera, M.C, and Saadoun, A, Bioaccessibility of Se, Cu, Zn, Mn and Fe, and haem iron content in unaged and aged meat of Hereford and Braford steers fed, Meat Science, 2012, 91, 116-124.
[18]	Terevinto, A, Oxidación lipídica y proteica, capacidad antioxidatuva y actividad de las enzimas catalasa, superóxido dismutasa y glutatión peroxidasa eb la carne fresca y madurada de novillos Hereford y Bradford, Tesis de Maestria en Ciencias Agrarias. Facultad de Agronomía, Uruguay,2010.
[19]	Cabrera, M.C, and Saadoun, A, An overview of the nutritional value of beef and lamb meat from South America, Meat Science, 2014, 98, 3, 435-444.