Proteomic Analysis of Human Breast Cancer Cells Treated with Monascus-Fermented Red Mold Rice Extracts

Chu-I Lee¹, Shu-Ling Hsieh², Chih-Chung Wu³, Chun-Lin Lee⁴, Yueh-Ping Chang⁵ and Jyh-Jye Wang^5,

¹Department of Medical Laboratory Science and Biotechnology, Fooyin University, Jinxue Rd., Daliao Dist., Kaohsiung, Taiwan

²Department of Seafood Sciences, National Kaohsiung Marine University, Haijhuan Rd., Nanzih Dist., Kaohsiung City, Taiwan

³Department of Nutrition and Health Sciences, Chang Jung Christian University, No.1,Changda Rd., Gueiren District, Tainan City, Taiwan

⁴Department of Life Science, National Taitung University, Sec.1 Chunghua Rd., Taitung, Taiwan

⁵Department of Nutrition and Health Science, Fooyin University, Jinxue Rd., Daliao Dist., Kaohsiung, Taiwan

Cite this paper:
Chu-I Lee, Shu-Ling Hsieh, Chih-Chung Wu, Chun-Lin Lee, Yueh-Ping Chang and Jyh-Jye Wang. Proteomic Analysis of Human Breast Cancer Cells Treated with Monascus-Fermented Red Mold Rice Extracts. Journal of Food and Nutrition Research. 2015; 3(5):325-329. doi: 10.12691/jfnr-3-5-6

Abstract

Monascus-fermented red mold rice (RMR) extracts have recently been proved to possess anticancer activities, but the anticancer effect of breast cancer cells treated with RMR is not yet well understood. In an effort to identify proteins that may be involved in RMR-induced breast cancer cell death, we employed proteomic analysis coupled with LC-nanoESI-MS/MS on MCF-7 cells exposed to two RMR extracts, ethyl acetate extract (EAE) and ethanol extract (EE). Sixupregulated proteins were identified in EAE-induced MCF-7 cells, including glyceraldehyde-3-phosphate dehydrogenase, pyruvate dehydrogenase E1 component, heat shock 27 kDa protein 1, cathepsin D, protein disulphideisomerase A3, and prohibitin. The upregulated expression of other 4 proteins was seen in EE-induced MCF-7 cells, including alloalbuminvenezia, annexin A5, endoplasmic reticulum protein 29 isoform 1 precursor, and cellular retinoic acid binding protein 2. The same two down-regulated proteins were identified on cells after treatment with both extracts, dermcidin preproprotein and poly(rC) binding protein 1. These proteins have been implicated in proapoptotic regulation, stress modulation, tumor suppression, and survival factor activity. These analysescould provide valuable information for further exploration of RMR as a promising chemopreventive agent against breast cancer.

Keywords:
Breast cancer cells Monascuspurpureus Proteomics Red mold riceextracts

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