Screening and Extracting Mycocin Secreted by Yeast Isolated from Koumiss and Their Antibacterial Effect

Yujie Chen¹, Chen Aorigele^1, , Chunjie Wang², Huasai Simujide¹ and Siqin Yang¹

¹College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China

²College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China

Cite this paper:
Yujie Chen, Chen Aorigele, Chunjie Wang, Huasai Simujide and Siqin Yang. Screening and Extracting Mycocin Secreted by Yeast Isolated from Koumiss and Their Antibacterial Effect. Journal of Food and Nutrition Research. 2015; 3(1):52-56. doi: 10.12691/jfnr-3-1-9

Abstract

Koumiss is a common fermented mare’s milk with beneficial therapeutic effects on cardiovascular disease, tuberculosis, and diarrhea. The mare’s milk is fermented by lactic acid bacteria and yeasts. Although information about LAB from Koumiss is comprehensive, there is limited knowledge about yeasts from Koumiss and their effects. The purpose of this study was to screen and extract mycocin secreted by yeast isolated from Koumiss and test their antibacterial effect against pathogenic Escherichia coli, The yeasts from Koumiss were isolated and those producing mycocin were screened by the Oxford cup method. Crude extracts of mycocin were then extracted by ethyl acetate, and temperature stabilities of them were investigated. The crude extracts of mycocin were tested against pathogenic E. coli and compared both in vivo and in vitro. Three Saccharomyces cerevisiae, and two Kluyveromyces marxianus were isolated from Koumiss in Inner Mongolia. All these yeasts produced mycocin. The two crude extracts of mycocin secreted by K. marxianus were active and stable at temperatures between 25°C and 45°C. They had better antibacterial effect in vitro and in vivo and were shown to be effective in preventing E. coli disease in mice. It may be possible to use crude extracts of mycocin secreted by yeast isolated from Koumiss to inhibit the growth of E. coli.

Keywords:
Koumiss yeast escherichia coli mycocin

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References:

[1]	Steinkraus, K.H., Introduction to indigenous fermented foods. In K. H. Steinkraus , Handbook of indigenous fermented foods, Marcel Dekker, New York, 1-6. 1996.
[2]	Simango, C., “Potential use of traditional fermented foods for weaning in Zimbabwe”. Social Science Medicine, 44: 1065-1068. April. 1997.
[3]	3. Liu, S.N., Han, Y., and Zhou, Z.J., “Lactic acid bacteria in traditional fermented Chinese foods”. Food Research International, 44: 643-651. 2011.
[4]	Danova S., Petrov K., Pavlov P., and Petrova, P., “Isolation and characterization of Lactobacillus strains involved in koumiss fermentation”. International Journal of Dairy Technology, 58(2): 100-105. May. 2005.
[5]	Kabak, B., and Dobson, A.D.W., “An Introduction to the Traditional Fermented Foods and Beverages of Turkey”. Critical Reviews in Food Science and Nutrition, 51: 248-260. 2011.
[6]	He, Y. F., “Studies on Isolation, Identification of Microorganisms and Their Antibacterial Factors from Koumiss”. Inner Mongolia Agriculture University Doctoral Dissertation (Chinese), 2008.
[7]	Viljoen, B., “Yeast ecological interactions. Yeast’yeast, yeast’bacteria, yeast’fungi interactions and yeasts as biocontrol agents,” in Yeasts in Food and Beverages, eds. A. Querol and G. Fleet, Springer, Berlin, 83-110. 2006.
[8]	Golubev W.I., Mycocins Killer toxins. In: Kurtzman C.P. and Fell J.W. eds, edn. Elsevier, Amsterdam, The Netherlands, 55-62. 1998.
[9]	Barnett J.A., Payne R.W., and Yarrow D., Yeasts: Characteristics and Identification, 3rd edn. Cambridge University Press, Cambridge. 2000.
[10]	Zhang G.W., Hu M.M., He L., Fu P., Wang L., and Zhou J. “Optimization of microwave-assisted enzymatic extraction of polyphenols from waste peanut shells and evaluation of its antioxidant and antibacterial activities in vitro”. Food and Bioproducts Processing, 91: 158-168. 2013.
[11]	Yang E., Fan L.H., Jiang Y.M., Doucette C., and Fillmore S., “Antimicrobial activity of bacteriocin-producing lactic acid bacteria isolated from cheeses and yogurts”. AMB Express, 2:48. September. 2012.
[12]	Skovgaard, S., Larsen, M.H., Nielsen L.N., Skov R.L., Wong C., Westh H., and Ingmer H., “Recently introduced qacA/B genes in Staphylococcus epidermidis do not increase chlorhexidine MIC/MBC”. Journal of Antimicrobial Chemotherapy, 68: 2226-2233. June. 2013.
[13]	Zhang, J.R., Yao, X.K., Tan X.H., Liu H., Xiong S.Y., Li J., and Zhang G.Q., “Isolation and Identification of Yeast in Koumiss”. Xinjiang Agricultural Sciences (Chinese), 44: 206-211. 2007.
[14]	Ni, H.J., Bao, Q.H., Sun T.S., Chen X., and Zhang H.P., “Identification and biodiversity of yeasts isolated from Koumiss in Xinjiang of China”, Acta Microbiolgica Sinica (Chinese), 47: 578-582. 2007.
[15]	Shuangquan., Burentegusi., Yu, B., and Miyamoto, T., “Microflora in traditional starter cultures for fermented milk, hurunge, from Inner Mongolia, China”. Animal Science Journal, 77: 235-241. April. 2006.
[16]	Hatoum R., Labrie S., and Fliss I., “Antimicrobial and probiotic properties of yeasts: from fundamental to novel applications”. Frontiers in Microbiology, 3:421. December. 2012.
[17]	Suzuki, C., Ando, Y., and Machida, S., “Interaction of SMKT, a killer toxin produced by Pichia farinosa, with the yeast cell membranes”. Yeast, 18(16): 1471-1478. December. 2001.
[18]	Middelbeek, E.J., Hermans, J.M.H., and Stumm, C., “Production, purification and properties of a Pichia kluyveri killer toxin”. Antonie van Leeuwenhoek, 45(3):437-450. 1979.