Anti-Proliferative Effects in Human Breast Cancer MDA. MCF-7 Cells & Human Breast Epithelial MCF-10a Cells and Western Blot Analysis from Adlay (Coix Lacryma-Jobi L.) Varieties Phenolic Extracts

Lifeng Wang^{1, 2,} , Huihui Xie¹, Yumei Wang¹, Ruihai Liu² and Xingrong Ju¹

¹School of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing, Jiangsu PR, China

²Department of Food Science, Cornell University, Ithaca, NY

Cite this paper:
Lifeng Wang, Huihui Xie, Yumei Wang, Ruihai Liu and Xingrong Ju. Anti-Proliferative Effects in Human Breast Cancer MDA. MCF-7 Cells & Human Breast Epithelial MCF-10a Cells and Western Blot Analysis from Adlay (Coix Lacryma-Jobi L.) Varieties Phenolic Extracts. Journal of Food and Nutrition Research. 2014; 2(11):792-799. doi: 10.12691/jfnr-2-11-6

Abstract

Analyzed the anti-proliferative activities (EC₅₀ values) and cytotoxicities (CC₅₀ values) of adlay extracts with adlay (Coix lacryma-jobi L.) varieties grains, Guizhou Heigu (hard hull, black), Liaoning 5 (soft hull, black) and Longyi 1 (hard hull, brown) to be the materials, normal human MCF-10a breast epithelial cells, human MDA breast cancer cells and human MCF-7 breast cancer cells to be the research subjects. After being processed by free or bound phenolic extracts of adlay, detected the expression levels of proteins such as PCNA, p21 and CDK4, which were related to cell proliferation and cell cycle in cells, by using western-blot test to preliminary obtain the mechanism of inhibition of tumor cells proliferation of adlay extracts. The results showed that the adlay extracts by different varieties had obvious anti-proliferative effects to tumor cells, and adlay samples themselves had no cytotoxicity in the tested experimental concentration range. Besides, adlay extracts inhibited the proliferation of HepG2 cells by regulating the cell cycle mediated by DNA polymerase adjustment factors such as PCNA and p21.

Keywords:
adlay polyphenol cytotoxicity anti-proliferative activity protein expression

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