Effect of Solvents on the Antioxidant Activity of Walnut (Juglans regia L.) Shell Extracts

Jiong Yang¹, Chaoyin Chen^1, , Shenglan Zhao², Feng Ge¹ and Diqiu Liu¹

¹Faculty of life science, Kunming University of Science and Technology, Kunming, People's Republic of China

²Yunnan University of Traditional Chinese Medicine, Kunming, People's Republic of China

Cite this paper:
Jiong Yang, Chaoyin Chen, Shenglan Zhao, Feng Ge and Diqiu Liu. Effect of Solvents on the Antioxidant Activity of Walnut (Juglans regia L.) Shell Extracts. Journal of Food and Nutrition Research. 2014; 2(9):621-626. doi: 10.12691/jfnr-2-9-15

Abstract

Walnut shell is a waste generated in the walnut (Juglans regia L.) harvest, containing natural compounds with antioxidant properties. In this connection, the effect of the solvent (water, chloroform, methanol, ethanol, ethyl acetate and N-butanol) on the extraction yields, total flavonoids content and antioxidant properties was analyzed. Total flavonoids content of extracts was determined by NaNO2-Al(NO3)3 method. Extract antioxidant activity was measured by hydroxyl, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide anion radical scavenging capacity and the total antioxidant capacity and reducing power assay. The highest extraction yield was achieved with N-butanol (4.54%) and the greatest total flavonoids content shown by the samples extracted with ethyl acetate. The highest ability to scavenging DPPH, hydroxyl and superoxide radicals was ethyl acetate extract (EC50=81.03μg/mL), methanol extract (EC50=131.35 μg/mL) and chloroform extract (EC50=176.35μg/mL) respectively under the concentration of 500 μg/mL. The methanol extract showed the greatest total antioxidant activity and reducing power. The results showed that different solvent had significant effect on total flavonoids content and antioxidant activity. The result obtained demonstrated the potential of walnut shell as a source of antioxidant.

Keywords:
walnut shell solvent extraction total flavonoids content antioxidant activity

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References:

[1]	Srinivasan A, Viraraghavan T. Removal of oil by walnut shell media. Bioresource Technology, 2008, 99: 8217-8220.
[2]	Zabihi M, Haghighi Asl A, Ahmadpour A. Studies on adsorption of mercury from aqueous solution on activated carbons prepared from walnut shell. Journal of Hazardous Materials, 2010, 174: 251-256.
[3]	Akbari V, Jamei R, Heidari R, et al. Antiradical activity of different parts of Walnut (Juglans regia L.) fruit as a function of genotype. Food Chemistry, 2012, 135(4): 2404-2410.
[4]	Sultana B, Anwar F, Ashraf M. Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules, 2009, 14: 2167-2180.
[5]	Belmekki N, Bendimerad N. Antioxidant activity and phenolic content in methanol crude extracts from three Lamiaceae grown in southwestern Algeria. Carcinogenesis, 2012, 4: 6.
[6]	Akkol E K, Orhan I E, Yeşilada E. Anticholinesterase and antioxidant effects of the ethanol extract, ethanol fractions and isolated flavonoids from Cistus laurifolius L. leaves. Food Chemistry, 2012, 131: 626-631.
[7]	Fernández-Agulló A, Pereira E, Freire M S, et al. Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts. Industrial Crops and Products, 2013, 42: 126-132.
[8]	Zakaria Z A, Mohamed A M, Jamil N S M, et al. In vitro cytotoxic and antioxidant properties of the aqueous, chloroform and methanol extracts of Dicranopteris linearis leaves. African journal of Biotechnology, 2011, 10: 273-282.
[9]	Lateef M, Iqbal L, Fatima N, et al. Evaluation of antioxidant and urease inhibition activities of roots of Glycyrrhiza glabra. Pakistan Journal of Pharmaceutical Sciences, 2012, 25: 99-102.
[10]	Kurian G A, Suryanarayanan S, Raman A, et al. Antioxidant effects of ethyl acetate extract of Desmodium gangeticum root on myocardial ischemia reperfusion injury in rat hearts. Chinese Medicine, 2010, 5: 1-7.
[11]	Oliveira I, Sousa A, Ferreira I C F R, et al. Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglans regia L.) green husks. Food and Chemical Toxicology, 2008, 46: 2326-2331.
[12]	Masuoka N, Matsuda M, Kubo I. Characterisation of the antioxidant activity of flavonoids. Food Chemistry, 2012, 131: 541-545.
[13]	Cushnie T P, Lamb A J. Recent advances in understanding the antibacterial properties of flavonoids. International Journal of Antimicrobial Agents, 2011, 38: 99-107.
[14]	Fu Y, Chen J, Li Y J, et al. Antioxidant and anti-inflammatory activities of six flavonoids separated from licorice. Food Chemistry, 2013, 141: 1063-1071.
[15]	Cao J, Xia X, Chen X, et al. Characterization of flavonoids from Dryopteris erythrosora and evaluation of their antioxidant, anticancer and acetylcholinesterase inhibition activities. Food and Chemical Toxicology, 2013, 51: 242-250.
[16]	Li X, Chen W, Chen D. Protective effect against hydroxyl-induced DNA damage and antioxidant activity of Radix Glycyrrhizae (Liquorice Root). Advanced Pharmaceutical Bulletin, 2013, 3: 167-173
[17]	Islam F, Quadery T M, Chowdhury S R, et al. Antioxidant and Cytotoxic Activities of Mussaenda macrophylla. Bangladesh Pharm, 2012, 15: 69-71.
[18]	Chen N, Yang H, Sun Y, et al. Purification and identification of antioxidant peptides from walnut (Juglans regia L.) protein hydrolysates. Peptides, 2012, 38: 344-349.
[19]	Hu T, Liu D, Chen Y, et al. Antioxidant activity of sulfated polysaccharide fractions extracted from Undaria pinnitafida in vitro. International journal of biological macromolecules, 2010, 46: 193-198.
[20]	Chaouche T M, Haddouchi F, Ksouri R, et al. Antioxidant activity profiling by spectrophotometric methods of phenolic extract of Prasium majus L. Free Radicals and Antioxidants, 2013, 3: 43-46.
[21]	Lue B M, Nielsen N S, Jacobsen C, et al. Antioxidant properties of modified rutin esters by DPPH, reducing power, iron chelation and human low density lipoprotein assays. Food Chemistry, 2010, 123: 221-230.
[22]	Alothman M, Bhat R, Karim A A. Antioxidant capacity and phenolic content of selected tropical fruits from Malaysia, extracted with different solvents. Food Chemistry, 2009, 115: 785-788.
[23]	Sahreen S, Khan M R, Khan R A. Evaluation of antioxidant activities of various solvent extracts of Carissa opaca fruits. Food chemistry, 2010, 122: 1205-1211.
[24]	Sajid Z I, Anwar F, Shabir G, et al. Antioxidant, antimicrobial properties and phenolics of different solvent extracts from bark, leaves and seeds of Pongamia pinnata (L.) Pierre. Molecules, 2012, 17: 3917-3932.
[25]	Sahreen S, Khan M R, Khan R A. Evaluation of antioxidant activities of various solvent extracts of Carissa opaca fruits. Food chemistry, 2010, 122: 1205-1211.
[26]	Chaleshtori R S, Chaleshtori F S, Rafieian M. Biological characterization of Iranian walnut (Juglans regia) leaves. Turkish Journal of Biology, 2011, 35: 635-639.
[27]	Valko M, Leibfritz D, Moncol J, et al. Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry & Cell Biology, 2007, 39: 44-84.
[28]	Wei Q, Ma X, Zhao Z, et al. Antioxidant activities and chemical profiles of pyroligneous acids from walnut shell. Journal of analytical and applied pyrolysis, 2010, 88: 149-154.
[29]	Lee J, Koo N, Min D B. Reactive oxygen species, aging, and antioxidative nutraceuticals. Comprehensive Reviews in Food Science and Food Safety, 2004, 3(1): 21-33.
[30]	Li Y, Jiang B, Zhang T, et al. Antioxidant and free radical-scavenging activities of chickpea protein hydrolysate (CPH). Food Chemistry, 2008, 106: 444-450.
[31]	Loganayaki N, Siddhuraju P, Manian S. Antioxidant activity and free radical scavenging capacity of phenolic extracts from Helicteres isora L. and Ceiba pentandra L. Journal of Food Science and Technology, 2013, 50: 687-695.
[32]	Ak T, Gülçin İ. Antioxidant and radical scavenging properties of curcumin. Chemico-Biological Interactions, 2008, 174: 27-37.
[33]	Yen G C, Duh P D. Scavenging effect of methanolic extracts of peanut hulls on free-radical and active-oxygen species. Journal of Agricultural and Food Chemistry, 1994, 42: 629-632.
[34]	Habib H M, Ibrahim W H, Schneider-Stock R, et al. Camel milk lactoferrin reduces the proliferation of colorectal cancer cells and exerts antioxidant and DNA damage inhibitory activities. Food Chemistry, 2013, 141: 148-152.
[35]	Ren J, Zhao M, Shi J, et al. Purification and identification of antioxidant peptides from grass carp muscle hydrolysates by consecutive chromatography and electrospray ionization-mass spectrometry. Food Chemistry, 2008, 108: 727-736.