Determination of Antioxidant Activity in Garlic (Allium sativum) Extracts Subjected to Boiling Process in vitro

Cunbao Liu¹, Xu Yang¹, Yufeng Yao¹, Weiwei Huang¹, Wenjia Sun¹ and Yanbing Ma^1,

¹Department of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, China

Cite this paper:
Cunbao Liu, Xu Yang, Yufeng Yao, Weiwei Huang, Wenjia Sun and Yanbing Ma. Determination of Antioxidant Activity in Garlic (Allium sativum) Extracts Subjected to Boiling Process in vitro. Journal of Food and Nutrition Research. 2014; 2(7):383-387. doi: 10.12691/jfnr-2-7-9

Abstract

Garlic is a vegetable known to be a good antioxidant food resource around the world. Several studies on the antioxidant activity of garlic, mainly conducted using one or two methods, have been reported. However, comparison of the antioxidant activity of garlic before and after cooking is rarely reported. In this study, we compared the antioxidant activity of garlic aqueous and methanol extracts processed before and after boiling to mimic the cooking process. By testing the antioxidant activities of the extracts in different chemical mimic systems in vitro, namely, ABTS [2, 2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid)] and DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging activities, reducing power, and metal chelating ability, we found the following data: (1) no significant difference was observed on the ABTS radical scavenging activities of garlic aqueous and methanol extracts before and after boiling process; (2) the reducing power of garlic aqueous and methanol extracts decreased by 25.9% and 14.1%, respectively, whereas the metal chelating activity of boiled garlic aqueous extracts increased by 54.7%; and (3) DPPH radical scavenging test may not be suitable to examine the garlic extracts. In addition, the ABTS radical scavenging activities of garlic extracts were very stable at pH ranges similar in human bodies, and both sulfhydryl and phenolic compounds were probably responsible for the antioxidant ability of garlic. The boiling process destroyed only a small part of garlic bio-ingredients related to antioxidant activity properties.

Keywords:
aqueous methanol extract radical scavenge reducing power metal chelating

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References:

[1]	Rajendran, P., Nandakumar, N., Rengarajan, T., Palaniswami, R., Gnanadhas, E.N., Lakshminarasaiah, U., Gopas, J., Nishigaki, I., “Antioxidants and human diseases”, Clin Chim Acta 14. 262-269. Jun. 2014.
[2]	Boveris, A., Chance, B., “The mitochondrial generation of hydrogen peroxide. General properties and effect of hyperbaric oxygen”, Biochem J 134. 707-716. 1973.
[3]	Ryan, B.J., Nissim, A., Winyard, P.G., “Oxidative post-translational modifications and their involvement in the pathogenesis of autoimmune diseases”, Redox Biol 28. 715-724. May. 2014.
[4]	Aseervatham, G.S., Sivasudha, T., Jeyadevi, R., Arul, Ananth.D., “Environmental factors and unhealthy lifestyle influence oxidative stress in humans--an overview”, Environ Sci Pollut Res Int 20. 4356-4369. Jul. 2013.
[5]	Hancock, J.T., Desikan, R., Neil, S.J., “Role of reactive oxygen species in cell signaling pathways”, Biochem Soc Trans 29. 345-350. 2001.
[6]	Schieber, M., Chandel, N.S., “ROS function in redox signaling and oxidative stress”, Curr Biol 24. R453-R462. May 2014.
[7]	Wanagat, J., Dai, D.F., Rabinovitch, P., “Mitochondrial oxidative stress and mammalian healthspan”, Mech Ageing Dev 131. 527-535. Jul-Aug. 2010.
[8]	Dai, D.F., Chiao, Y.A., Marcinek, D.J., Szeto, H.H., Rabinovitch, P.S., “Mitochondrial oxidative stress in aging and healthspan”, Longev Healthspan 3. 6. May. 2014.
[9]	Podda, M., Grundmann-Kollmann, M., “Low molecular weight antioxidants and their role in skin ageing”, Clin Exp Dermatol 26. 578-582. Oct. 2001.
[10]	Kohen, R., Gati, I., “Skin low molecular weight antioxidants and their role in aging and oxidative stress”, Toxicology 148. 149-157. 2000.
[11]	Kozina, L.S., Borzova, I.V., Arutiunov, V.A., Ryzhak, G.A., “The role of oxidative stress in skin aging”, Adv Gerontol 25. 217-222. 2012.
[12]	Vilela, F.M., Fonseca, Y.M., Jabor, J.R., Vicentini, F.T., Fonseca, M.J., “Effect of ultraviolet filters on skin superoxide dismutase activity in hairless mice after a single dose of ultraviolet radiation”, Eur J Pharm Biopharm 80. 387-392. Feb. 2012.
[13]	Mathew, S., Abraham, T.E., “In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies”, Food Chem Toxicol 44. 198-206. 2006.
[14]	Mathew, S., Abaraham, T.E., “Studies on the antioxidant activities of cinnamon (Cinnamomum verum) bark extracts, through various in vitro models”, Food Chemistry 94. 520-528. 2006.
[15]	Oyaizu, M., “Studies on product of browning reaction prepared from glucose amine”, J Nutr 44. 307-315. 1986.
[16]	Dinis, T.C., Maderia, V.M., Almeida, L.M., “Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers:, Arch Biochem Biophys 315. 161-169. 1994.
[17]	Childs, R.E., Bardsley, W.G., “The steady-state kinetics of peroxidase with 2,2’-azino-di-(3-ethyl- benzthiazoline-6-sulphonic acid) as chromogen”, Biochem J 145. 93-103. 1975.
[18]	Shin, T., Murao, S., Matsumura, E., “A chromogenic oxidative coupling reaction of laccase: applications for laccase and angiotensin I converting enzyme assay”, Anal Biochem 166. 380-388. 1987.
[19]	Akerström, B., Maghzal, G.J., Winterbourn, C.C., Kettle, A.J., “The lipocalin alpha1-microglobulin has radical scavenging activity”, J Biol Chem 282. 31493-31503. 2007.
[20]	Foti, M.C., Daquino, C., Geraci, C., “Electron-transfer reaction of cinnamic acids and their methyl esters with the DPPH(*) radical in alcoholic solutions”, J Org Chem 69. 2309-2314. 2004.
[21]	Magalhães, L.M., Segundo, M.A., Reis, S., Lima, J.L., Tóth, I.V., Rangel, A.O., “Automatic flow system for sequential determination of ABTS*+ scavenging capacity and Folin-Ciocalteu index: a comparative study in food products”, Anal Chim Acta 592. 193-201. 2007.
[22]	Stasko, A., Brezová, V., Biskupic, S., Misík, V., “The potential pitfalls of using 1,1-diphenyl-2-picrylhydrazyl to characterize antioxidants in mixed water solvents” Free Radic Res 41. 379-390. 2007.
[23]	Yin, M.C., Hwang, S.W., Chan, K.C., “Nonenzymatic antioxidant activity of four organosulfur compounds derived from garlic”, J Agric Food Chem 50. 6143-6147. 2002.
[24]	Matsudaira, P., “Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes”, J Biol Chem 262. 10035-10038. 1987.