Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: https://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2024, 12(10), 461-470
DOI: 10.12691/jfnr-12-10-8
Open AccessArticle

Characterization of the Key Flavor Compounds Contributing to the Lingering Cup Aroma in Sauce-Flavor Baijiu Using Sensomics and Flavoromics

Nian Cao1, Yubo Yang1, Li Wang1 and Fan Yang1,

1Kweichow Moutai Co., Ltd

Pub. Date: October 30, 2024

Cite this paper:
Nian Cao, Yubo Yang, Li Wang and Fan Yang. Characterization of the Key Flavor Compounds Contributing to the Lingering Cup Aroma in Sauce-Flavor Baijiu Using Sensomics and Flavoromics. Journal of Food and Nutrition Research. 2024; 12(10):461-470. doi: 10.12691/jfnr-12-10-8

Abstract

This study utilized sensomics and flavoromics approaches to identify the primary flavor compounds contributing to the lingering cup aroma of sauce-flavor Baijiu (SFB). 15 odor-active compounds were detected through gas chromatography–olfactometry-mass spectrometry (GC–O-MS) combined with odor activity value (OAV) analysis. A positive correlation was observed between the price and cup aroma durability across 30 SFB samples, leading to the identification of 17 compounds as potential markers with high variable importance in projection (VIP) values (VIP ≥ 1). Finally, aroma recombination and omission tests demonstrated that 17 aroma compounds were crucial for the lingering cup aroma, while four higher fatty acid ethyl esters and L-lactic acid contributed to the durability by enhancing substance interactions within the system, sustaining presence of aroma compounds in the cup.

Keywords:
Sauce-flavor Baijiu lingering cup aroma gas chromatography–olfactometry-mass spectrometry (GC–O-MS) odor activity value (OAV) variable importance in projection (VIP) aroma recombination

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]  Wang, L.; Hu, G.Y.; Lei, L.B.; Lin, L.; Wang, D.Q.; Wu, J.X. Identification and Aroma Impact of Volatile Terpenes in Moutai Liquor. Int. J. Food Prop. 2016, 19, 1335-1352.
 
[2]  Wang, M.Y.; Yang, J.G.; Zhao, Q.S.; Zhang, K.Z.; Su, C. Research Progress on Flavor Compounds and Microorganisms of Maotai Flavor Baijiu. Journal of Food Science 2019, 84, 6-18.
 
[3]  Zhang J.; Huang, J.L.; Zhao, Y.M.; Tian, Z.Q. Study on the Determination of Volatile Flavoring Components in Jiangxiang Baijiu (Liquor) by Static Headspace Gas Chromatography. LiqourMaking Science Technology 2014, 104-106.
 
[4]  Li, S.P. Construction of Flavor Wheel of Offensive Odors in Jiangxiang Baijiu and Study on its Sensory Characteristics. LiqourMaking Science Technology 2022, 84-89.
 
[5]  CaO, S.S. Discussion on flavor components of Maotai-flavored liquor. LiqourMaking Science Technology 1991, 47-48.
 
[6]  Wu, H.Q.; Zhang, G.Y.; H, S.M.; Jaing, H.B.; Cheng, Z.Q. Study on Aroma components of Maotai-flavored liquor ─2. GC/MS analysis of fragrance components in cup aroma of Zhenjiu and Maotai. Journal of Analytical Testing 1996, 5-8.
 
[7]  Sun, W.J.; Wang, T.C.; Lei, Y.; Liu, H.G.; Deng, Y.H. Lingering aroma composition in empty glass of sauce-flavor Baijiu by SHS-GC/MS. China Brewing 2021, 40, 93-97.
 
[8]  Qin, D.; Lv, S.L.; Shen, Y.; Shi, J.; Jiang, Y.L.; Cheng, W.; Wang, D.M.; Li, H.H.; Zhang, Y.Y.; Cheng, H.; et al. Decoding the key compounds responsible for the empty cup aroma of soy sauce aroma type baijiu. Food Chem. 2024, 434, 12.
 
[9]  Qin, D.; Wu, Z.Y.; Shen, Y.; Cheng, H.; Yang, K.; Li, H.H.; Ye, X.Q.; Sun, B.G. Characterization of empty cup aroma in Soy sauce aroma type Baijiu by vacuum assisted sorbent extraction. J. Food Compos. Anal. 2023, 117, 12.
 
[10]  Vrzal, T.; Olsovská, J. Sensomics - basic principles and practice. Kvasny Prumysl 2019, 65, 166-173.
 
[11]  Huang, M.Q.; Huo, J.Y.; Wu, J.H.; Zhao, M.M.; Sun, J.Y.; Zheng, F.P.; Sun, X.T.; Li, H.H. Structural characterization of a tetrapeptide from Sesame flavor-type Baijiu and its interactions with aroma compounds. Food Res. Int. 2019, 119, 733-740.
 
[12]  Fan, H.Y.; Fan, W.L.; Xu, Y. Characterization of Key Odorants in Chinese Chixiang Aroma-Type Liquor by Gas Chromatography-Olfactometry, Quantitative Measurements, Aroma Recombination, and Omission Studies. J. Agric. Food Chem. 2015, 63, 3660-3668.
 
[13]  Schuh, C.; Schieberle, P. Characterization of the key aroma compounds in the beverage prepared from Darjeeling black tea: Quantitative differences between tea leaves and infusion. J. Agric. Food Chem. 2006, 54, 916-924.
 
[14]  Wang, G.N.; Song, X.B.; Zhu, L.; Li, Q.; Zheng, F.P.; Geng, X.J.; Li, L.H.; Wu, J.H.; Li, H.H.; Sun, B.G. A flavoromics strategy for the differentiation of different types of Baijiu according to the non-volatile organic acids. Food Chem. 2022, 374, 10.
 
[15]  Perestrelo, R.; Silva, C.; Câmara, J.S. A useful approach for the differentiation of wines according to geographical origin based on global volatile patterns. J. Sep. Sci. 2014, 37, 1974-1981.
 
[16]  Murray, J.M.; Delahunty, C.M.; Baxter, I.A. Descriptive sensory analysis: past, present and future. Food Res. Int. 2001, 34, 461-471.
 
[17]  Stone, H.; Sidel, J.; Oliver, S.; Woolsey, A.; Singleton, R.C. Sensory evaluation by quantitative descriptive analysis; Descriptive Sensory Analysis in Practice: 1998.
 
[18]  Niu, Y.W.; Zhang, X.M.; Xiao, Z.B.; Song, S.Q.; Eric, K.; Jia, C.S.; Yu, H.Y.; Zhu, J.C. Characterization of odor-active compounds of various cherry wines by gas chromatography-mass spectrometry, gas chromatography-olfactometry and their correlation with sensory attributes. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 2011, 879, 2287-2293.
 
[19]  Zhu, J.C.; Chen, F.; Wang, L.Y.; Niu, Y.W.; Yu, D.; Shu, C.; Chen, H.X.; Wang, H.L.; Xiao, Z.B. Comparison of Aroma-Active Volatiles in Oolong Tea Infusions Using GC-Olfactometry, GC-FPD, and GC-MS. J. Agric. Food Chem. 2015, 63, 7499-7510.
 
[20]  Hu, S.W.; Zhang, G.J. Reading Liquor Analysis Methods (2007 Version). Liqour Making 2007, 91-92.
 
[21]  Gemert, L.J.V. Compilations of odour threshold values in air, water and other media. 2003.
 
[22]  Kun, J.R.; Meng, Q.; Wei, C.C.; Xie, G.H.; Yan, J.N.; Ho, C.T.; Tong, H.R. Characterization of the key compounds responsible for the fermented soybean-like cup aroma of raw Pu-erh tea using instrumental and sensory methods. LWT-Food Sci. Technol. 2022, 162, 8.
 
[23]  Cheng, Y.; Huynh-Ba, T.; Blank, I.; Robert, F. Temporal changes in aroma release of Longjing tea infusion: Interaction of volatile and nonvolatile tea components and formation of 2-butyl-2-octenal upon aging. J. Agric. Food Chem. 2008, 56, 2160-2169.
 
[24]  Schieberle, P.J.C.o.F. New Developments in Methods for Analysis of Volatile Flavor Compounds and their Precursors. 1995, 403-431.
 
[25]  Wu, Z.Y.; Yang, S.Q.; Xu, L.; Li, H.H.; Sun, J.Y.; Xu, Y.Q.; Huang, M.Q.; Sun, B.G. Screening and identifying microorganisms with feruloyl esterase activity in Chinese sesame-flavour baijiu fermentation materials (Jiupei). J. Food Compos. Anal. 2021, 102, 7.
 
[26]  Li, X.G.; Chen, J.B.; Wang, L.; Wang, D.Q.; Lei, L.B. Analysis of Organic Acids in Jiangxiang Baijiu. LiqourMaking Science Technology 2019, 105-107+111.
 
[27]  Wang, J.S.; Chen, H.; Wu, Y.S.; Zhao, D.R. Uncover the flavor code of strong-aroma baijiu: Research progress on the revelation of aroma compounds in strong-aroma baijiu by means of modern separation technology and molecular sensory evaluation. J. Food Compos. Anal. 2022, 109, 18.
 
[28]  Hou, X.Y; Gu, R.L; Liang, W.L;Xiao, Z.J. Research Progress on Production of Tetramethylpyrazine by Fermentation. Biotechnology Bulletin 2016, 32, 58-64.
 
[29]  Ciou, J.H.; Li, S.H.; Lee, P.S. Ti3C2 MXene Paper for the Effective Adsorption and Controllable Release of Aroma Molecules. SMALL 2019, 15.
 
[30]  Zhang, Q.; Shi, J.; Wang, Y.; Zhu, T.T.; Huang, M.Q.; Ye, H.; Wei, J.W.; Wu, J.H.; Sun, J.Y.; Li, H.H. Research on interaction regularities and mechanisms between lactic acid and aroma compounds of Baijiu. Food Chem. 2022, 397, 11.
 
[31]  Senses, G.J.C. Evaluation of the key odorants of foods by dilution experiments, aroma models and omission. Chemical Senses 2001, 26, 533-545.
 
[32]  Liu, Q.R.; Lin, X.L.; Lu, Z.M.; Chai, L.J.; Wang, S.T.; Shi, J.S.; Zhang, S.Y.; Shen, C.H.; Zhang, X.J.; Xu, Z.H. Influence on the volatilization of ethyl esters: Nonnegligible role of long-chain fatty acids on Baijiu flavor via intermolecular interaction. Food Chem. 2024, 436, 11.