Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: https://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2023, 11(3), 269-276
DOI: 10.12691/jfnr-11-3-12
Open AccessArticle

Sulforaphane (SFN) Exerts Anti-inflammatory Effects on LPS-stimulated RAW 264.7 and Mouse Bone Marrow Driven Macrophages by Modulating p65 Phosphorylation and Histone Acetylation

Seok-Yeong Yu1, 2, Zhenhua Liu1, Soonkyu Chung1 and Young-Cheul Kim1,

1Department of Nutrition, University of Massachusetts, Amherst, MA

2Olson Center for Women’s Health, Department of Obstetrics and Gynecology, College of Medicine, University of Nebraska Medical Center, Omaha, Nebraska

Pub. Date: March 27, 2023

Cite this paper:
Seok-Yeong Yu, Zhenhua Liu, Soonkyu Chung and Young-Cheul Kim. Sulforaphane (SFN) Exerts Anti-inflammatory Effects on LPS-stimulated RAW 264.7 and Mouse Bone Marrow Driven Macrophages by Modulating p65 Phosphorylation and Histone Acetylation. Journal of Food and Nutrition Research. 2023; 11(3):269-276. doi: 10.12691/jfnr-11-3-12

Abstract

Obesity-induced chronic inflammation contributes to the development of insulin resistance and type 2 diabetes and largely pertains to macrophages, primarily derived from bone marrow, (BMDMs) in insulin-sensitive tissues. Sulforaphane (SFN) is a major bioactive of cruciferous vegetables with a potent anti-inflammatory property. However, potential molecular modes of action remain unclear. The phosphorylation of nuclear factor-kappa B (NF-κB) and subsequent histone acetylation are critical signaling pathways for the transcription of pro-inflammatory genes in macrophages in obesity. Therefore, we tested our hypothesis that SFN mitigates the expression of pro-inflammatory genes in macrophages by suppressing p65 phosphorylation and histone acetylation using RAW 264.7 cell line and primary mouse BMDMs. In RAW 264.7 macrophages, SFN significantly inhibited the lipopolysaccharide (LPS)-induced pro-inflammatory genes expression, including iNos, Cox-2, Tnfα, Mcp-1 and Il-6, and suppressed CD11c immunofluorescence. Moreover, SFN significantly inhibited p65 phosphorylation and acetylation levels of histone H4, while it increased the histone deacetylase 3 (HDAC3) expression. Similarly, SFN inhibited p65 phosphorylation and histone H4 acetylation with HDAC3 expression increased in the BMDMs. These results revealed that SFN exerts anti-inflammatory effects via modulation of p65 phosphorylation and histone acetylation in macrophages. Our work suggests that supplementation of SFN or SFN-containing vegetables may serve as an anti-inflammatory diet component for mitigating obesity-related inflammation and related metabolic disorders.

Keywords:
Sulforaphane NF-κB RAW264.7 macrophage bone marrow-derived macrophage inflammation

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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