Efficacy of Doenjang, a Traditional Korean Fermented Food, against Dextran Sulfate Sodium-induced Ulcerative Colitis: Effect of Distribution of Microorganisms

Do-Youn Jeong¹, Hee-Jong Yang¹, Su-Ji Jeong¹, Dong-Yeop Shin², Young Mi Park², Hak Yong Lee² and Na-Rae Shin^2,

¹Microbial Institute for Fermentation Industry (MIFI), Sunchang, 56048, Republic of Korea

²INVIVO Co. Ltd. Daehak-ro 121, Nonsan 32992, Republic of Korea

Cite this paper:
Do-Youn Jeong, Hee-Jong Yang, Su-Ji Jeong, Dong-Yeop Shin, Young Mi Park, Hak Yong Lee and Na-Rae Shin. Efficacy of Doenjang, a Traditional Korean Fermented Food, against Dextran Sulfate Sodium-induced Ulcerative Colitis: Effect of Distribution of Microorganisms. Journal of Food and Nutrition Research. 2021; 9(12):657-663. doi: 10.12691/jfnr-9-12-6

Abstract

Doenjang, a traditional fermented food in Korea, has been consumed as a health food for a long time and is known worldwide. Doenjang has varying biological effects depending on the manufacturing method and fermentation or aging period. In this study, we confirmed the protective effect of Doenjang against ulcerative colitis (UC) according to distribution of microorganisms. In this experiment, a rat model of a dextran sulfate sodium (DSS)-induced UC was used. Doenjang (TSMM29 and TSMM30) and positive control (VSL#3; Desimone) were administered orally for 14 days before and after DSS induction. Our results revealed that the DSS-induced group showed decreased body weight, contraction of colon tissue, decreased colon weight, and increased inflammatory cytokine levels. On the contrary, in the TSMM-treated group, changes to body weight, colon tissue, and colon weight were recovered, and inflammatory cytokine levels were decreased. Histological results showed that the TSMM-treated group showed decreased edema and inflammatory cell infiltration, and recovery of the destroyed goblet cells In particular, the TSMM29 group showed the highest degree of recovery, thus showing that the distribution of microorganisms in Doenjang influences its biological effects in UC.

Keywords:
Doenjang Korea traditional fermented food microorganisms Ulcerative colitis Dextran sulfate sodium

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References:

[1]	Liu, S., Zhao, W., Lan, P., Mou, X., “The microbiome in inflammatory bowel diseases: from pathogenesis to therapy,” Protein & Cell. 12(5). 331-345. May. 2020.
[2]	Camos, F.G., Waitzberg, D.L., Teixeira, M.G., Mucerino, D.R., Habr-Gama, A. Kiss, D.R., “Inflammatory bowel diseases: principles of nutritional therapy” Revista do Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo 57(4). 187-198. Jul-Aug. 2002.
[3]	Lakatos, P.L., Lakatos, L., “Risk for colorectal cancer in ulcerative colitis: changes, causes and management strategies” World Journal of Gastroenterology 14(25). 3937-3947. Jul. 2008.
[4]	Abegunde, A.T., Muhammad, B.H., Bhatti, O., Ali, T., “Environmental risk factors for inflammatory bowel diseases: Evidence based literature review” World Journal of Gastroenterology 22(27). 6296-6317. Jul. 2016.
[5]	Peyrin-Biroulet, L., Panés, J., Sandborn W.J., Vermeire, S., Danese, S., Feagan B.G., et al., “Defining Disease Severity in Inflammatory Bowel Diseases: Current and Future Directions” Clinical Gastroenterology and Hepatology 14(3). 348-354. e17. Jun. 2016.
[6]	Yun, J., Xu, C.T., Pan, B.R., “Epidemiology and gene markers of ulcerative colitis in the Chinese” World Journal of Gastroenterology 15(7). 788-803. Fed. 2009.
[7]	Wadie, W., Abdel-Aziz, H., Zaki, H.F., Kellber, O., Weiser, D., Khayyal, M.T., “STW 5 is effective in dextran sulfate sodium-induced colitis in rats” International Journal of Colorectal Disease 27(11). 1445-1453. May. 2012.
[8]	Sun, B., Yuan, J., Wang, S., Lin, J., Zhang, W., Shao, J., et al., “Qingchang Suppository Ameliorates Colonic Vascular Permeability in Dextran-Sulfate-Sodium-Induced Colitis” Frontiers in Pharmacology 9. 1235. Oct. 2018.
[9]	Papadakis, K.A., Targan, S.R., “Role of cytokines in the pathogenesis of inflammatory bowel disease” Auunal Review of Medicine 51. 289-298. 2000.
[10]	Lee, M., Kovacs-Nolan, J., Yang, C., Archbold, T., Fan, M.Z., Mine, T., “Hen egg lysozyme attenuates inflammation and modulates local gene expression in a porcine model of dextran sodium sulfate (DSS)-induced colitis” J. Agric. Food. Chem. 57(6) 2233-2240. Mar. 2009.
[11]	Mager, L.F., Wasmer, M.H., Rau, T.T., Krebs, P., “Cytokine-Induced Modulation of Colorectal Cancer” Frontiers in Oncology. 6. 96. Apr. 2016.
[12]	Okayasu, M., Ogata, H., Yoshiyama, Y., “Use of corticosteroids for remission induction therapy in patients with new-onset ulcerative colitis in real-world settings” Journal of Market Access & Health Policy 7(1) 1565889. Jan. 2019.
[13]	Kozuch, P.L., Hanauer, S.B., “Treatment of inflammatory bowel disease: a review of medical therapy” World Journal of Gastroenterology 14(3). 354-377. Jan. 2008.
[14]	Chung, H.K., Yang, H.J., Shin, D., Chung, K.R., “Aesthetics of Korean foods: The symbol of Korean culture” Journal of Ethnic Foods 3(3). 178-188. Sep. 2016.
[15]	Jang, S.E., Kim, K.A., Han, M.J., Kim, D.H., “Doenjang, a fermented Korean soybean paste, inhibits lipopolysaccharide production of gut microbiota in mice” Jornal of Medicinal Food 17(1). 67-75. Jan. 2014.
[16]	Lee, J.E., Park, S.R., Lim, S.I., “Mass spectrometry-based metabolomic study of traditional Doenjang effects against hepatic fibrosis” Applied Biological Chemistry 59: 775-780. 2016.
[17]	Kang, S.J., Seo, J.Y., Cho, K.M., Lee, C.K., Kim, J.H., Kim, J.S., “Antioxidant and Neuroprotective Effects of Doenjang Prepared with Rhizopus, Pichia, and Bacillus” Preventive Nutrition and Food Science 21(3). 221-226. Sep. 2016.
[18]	Kim, T.W., Lee, J.H., Kim, S.E., Park, M.H., Chang, H.C., Kim, H.Y., “Analysis of microbial communities in doenjang, a Korean fermented soybean paste, using nested PCR-denaturing gradient gel electrophoresis” International Journal of Food Microbiology 131(2-3) 265-271. May. 2009.
[19]	Jeon, Y.D., Bang, K.S., Shin, M.K., Lee, J.H., Chang, Y.N., Jin, J.S., “Regulatory effects of glycyrrhizae radix extract on DSS-induced ulcerative colitis” BMC Complementary Medicine and Therapies 16(1) 459. Nov. 2016.
[20]	Shin, S.K., Cho, J.H., Kim, E.J., Kim, E.K., Park, D.K., Kwon, K.A., et al., “Anti-inflammatory and anti-apoptotic effects of rosuvastatin by regulation of oxidative stress in a dextran sulfate sodium-induced colitis model” World Journal of Gastroenterology 23(25) 4559-4568. Jul. 2017.
[21]	Wallace JL and Keenan CM: An orally active inhibitor of leukotriene synthesis accelerates healing in a rat model of colitis. Am. J. Physiol. 258: G527-G534. 1990.
[22]	Hendrickson, B.A., Gokhale, R., Cho, J.H., “Clinical Aspects and Pathophysiology of Inflammatory Bowel Disease” Clinical Microbiology Reviews 15(1). 79-94. Jan. 2002.
[23]	Jang, J.C., Lee, K.M., Ko, S.G., “Angelica acutiloba Kitagawa Extract Attenuates DSS-Induced Murine Colitis” Mediators of Inflammation 2016. 9275083. May. 2016.
[24]	Paine, E.R., “Colonoscopic evaluation in ulcerative colitis” Gastroenterol Report 2(3). 161-168. Aug. 2014.
[25]	Taghipour, N., Molaei, M., Mosaffa, N., Rostami-Nejad, M., Aghdaei, H.A., Anissian, A., et al., “An experimental model of colitis induced by dextran sulfate sodium from acute progresses to chronicity in C57BL/6 correlation between conditions of mice and the environment” Gastroenterology and Hepatology from Bed to Bench 9(1). 45-52. Winter. 2016.
[26]	Kwak, C.S., Son, D., Chung, Y.S., Kwon, Y.H., “Antioxidant activity and anti-inflammatory activity of ethanol extract and fractions of Doenjang in LPS-stimulated RAW 264.7 macrophages” Nutrition Research and Practice 9(6). 569-578. Dec. 2015.
[27]	Seol, J.Y., Youn, Y.N., Koo, M., Kim, H.J., Choi, S.Y., “Influence of water-soluble extracts of long-term fermented Doenjang on bone metabolism bioactivity and breast cancer suppression” Food Science and Biotechnology 25(2). 517-524. Apr. 2016.
[28]	Park, S.M., Oh, J., Kim, J.E., Kim, J.S., “Effect of Drying Conditions on Nutritional Quality and In Vitro Antioxidant Activity of Traditional Doenjang” Preventive Nutrition and Science 23(2). 144-151. Jun. 2018.
[29]	Yang, H.J., Kim, M.J., Kim, K.S., Lee, J.E., Hong, S.P., “In Vitro Antidiabetic and Antiobesity Activities of Traditional Kochujang and Doenjang and Their Components” Preventive Nutrition and Science 24(3). 274-282. Sep. 2019.
[30]	Kim, M.J., Kwak, H.S., Kim, S.S., “Effects of salinity on bacterial communities, Maillard reactions, isoflavone composition, antioxidation and antiproliferation in Korean fermented soybean paste (doenjang)” Food Chemistry 245. 402-409. Apr. 2018.
[31]	Kim, J.W., Doo, H.S., Kwon, T.H., Kim, Y.S., “Quality Characteristics of Doenjang Meju Fermented with Aspergillus Species and Bacillus subtilis during Fermentation” Korean J. Food Preserv. 18(3). 397-406. Jun. 2011.
[32]	Chen, S.J., Liu, X.W., Liu, J.P., Yang, X.Y., Lu, F.G., “Ulcerative colitis as a polymicrobial infection characterized by sustained broken mucus barrier” World Journal of Gastroenterology 20(28) 9468-9475. Jul. 2014.
[33]	Eichele, D.D., Kharbanda, K.K., “Dextran sodium sulfate colitis murine model: An indispensable tool for advancing our understanding of inflammatory bowel diseases pathogenesis” World Journal of Gastroenterology 23(33). 6016-6029. Sep. 2017.
[34]	Li, X., Liu, R., Zhao, Y., Gao, N., Jin, X., Gao, X., et al., “The extract from the roots of Rose odorata sweet var. gigantean (Coll. et Hemsl.) Rehd. et Wils attenuates DSS-induced ulcerative colitis by regulating the Nrf2/NF-κB signaling pathways” RSC Advances Journal 10(16). 9450-94601. 2020.
[35]	Al-Tamimi, A.A., Kadhim, K.H., Hussein, D.M., “Morphological and Histological Study of the Cecum and Colon in Adult Local Camelus dromedaries” Advances in Animal and Veterinary Sciences. 6. 7. Jul. 2018.
[36]	Saw, T.Y., Malik, N.A., Lim, K.P., Teo, C.W.L., Wong, E.S.M., Kong, S.C., “Oral Supplementation of Tocotrienol-Rich Fraction Alleviates Severity of Ulcerative Colitis in Mice” Journal of Nutritional Science and Vitaminology. 65(4) 318-327. 2019.
[37]	Jadhav, S.R., Shandilya, U.K., Kansal, V.K., “Immunoprotective Effect of Probiotic Dahi Containing Lactobacillus acidophilus and Bifidobacterium bifidum on Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice” Probiotics and Antimicrobial Proteins 4(1). 21-26. Mar. 2011.
[38]	Su, H., Kang, Q., Wang, H., Yin, H., Duan, L., Liu, Y., “Changes in expression of p53 and inflammatory factors in patients with ulcerative colitis” Experimental and Therapeutic Medicine 17(4). 32451-2456. Apr. 2019.
[39]	Kang, C.H., Choi, Y.H., Choi, I.W., Lee, J.D., Kim, G.Y., “Inhibition of Lipopolysaccharide-Induced iNOS, COX-2, and TNF-α Expression by Aqueous Extract of Orixa-Japonica in RAW 264.7 Cells via Suppression of NF-κB Activity” Tropical Journal of Pharmaceutical Research 10. 161. Nov. 2011.
[40]	Fausel, R., Afzali, A., “Biologics in the management of ulcerative colitis – comparative safety and efficacy of TNF-α antagonists” Therapeutics and Clinical Risk Managenment 11. 63-73. Jan. 2015.