Journal of Food and Nutrition Research
ISSN (Print): 2333-1119 ISSN (Online): 2333-1240 Website: https://www.sciepub.com/journal/jfnr Editor-in-chief: Prabhat Kumar Mandal
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Journal of Food and Nutrition Research. 2021, 9(3), 114-123
DOI: 10.12691/jfnr-9-3-3
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Determination of Phenolic Content, Biological Activity, and Enzyme Inhibitory Properties with Molecular Docking Studies of Rumex nepalensis, an Endemic Medicinal Plant

Ercan Bursal1, , Mustafa Abdullah Yılmaz2, Abdülmelik Aras3, Fikret Türkan4, Ümit Yildiko5, Ömer Kılıç6 and Abhijit Dey7

1Department of Nursing, Faculty of Health, Muş Alparslan University, Mus, Turkey

2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Dicle University, Diyarbakır, Turkey

3Department of Biochemistry, Faculty of Science and Arts, Igdır University, Igdır, Turkey

4Health Services Vocational School, Igdır University, Igdır, Turkey

5Department of Bioengineering, Kafkas University, Kars, Turkey

6Department of Pharmaceutical Sciences, Pharmacy Faculty, Adıyaman University, Adıyaman, Turkey

7Department of Life Sciences, Presidency University, Kolkata, India

Pub. Date: March 14, 2021

Cite this paper:
Ercan Bursal, Mustafa Abdullah Yılmaz, Abdülmelik Aras, Fikret Türkan, Ümit Yildiko, Ömer Kılıç and Abhijit Dey. Determination of Phenolic Content, Biological Activity, and Enzyme Inhibitory Properties with Molecular Docking Studies of Rumex nepalensis, an Endemic Medicinal Plant. Journal of Food and Nutrition Research. 2021; 9(3):114-123. doi: 10.12691/jfnr-9-3-3

Abstract

This study was conducted to evaluate the phenolic content, antioxidant potential, and enzyme inhibitiory properties of Rumex nepalensis by in vitro spectrophotometric methods. The experiments demonstrated that glutathione S-transferase (GST), α-glycosidase (α-Gly), acetylcholinesterase (AChE), and butyrylcholinesterase (BChE) enzymes were strongly inhibited by R. nepalensis extracts. The IC50 values for GST, α-Gly, AChE, and BChE enzyme inhibitions were calculated as 21.01 mg/mL, 34.65 mg/mL, 27.72 mg/mL, and 17.32 mg/mL, respectively. Also, effective antioxidant capacities of water and methanol extracts of R. nepalensis were determined by ABTS, CUPRAC, DPPH, and FRAP methods. Furthermore, quinic acid (15.61 mg/g), miquelianin (2.06 mg/g), quercitrin (1.97 mg/g), and protocatechuic acid (0.217 mg/g) were identified to be the major phenolic compounds of the plant extract according to the LC-MS/MS analysis. Finally, molecular docking studies were carried out to show the interactions of quinic acid, miquelianin, and quercitrin with AChE, BChE, GST, and α-Gly enzymes. Docking analysis indicated the possible roles of these phytochemicals in enzyme inhibitory activities.

Keywords:
biological activity enzyme inhibition molecular docking phenolic compounds Rumex

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