Microbiological Quality Assessment of Sashimi and Sushi Sold in the Federal District, Brazil

Karolina Oliveira Gomes¹, Flávia Bianca Amaral Alves¹, Ana Carolina Almeida de Oliveira Ferreira¹, Calliandra Maria de Souza Silva¹, Izabel Cristina Rodrigues da Silva¹ and Daniela Castilho Orsi^1,

¹University of Brasilia (UnB/FCE), Laboratory of Food Control, Centro Metropolitano, Conjunto A, lote 01, Ceilandia, CEP: 72220-900, Brasilia, DF, Brazil

Cite this paper:
Karolina Oliveira Gomes, Flávia Bianca Amaral Alves, Ana Carolina Almeida de Oliveira Ferreira, Calliandra Maria de Souza Silva, Izabel Cristina Rodrigues da Silva and Daniela Castilho Orsi. Microbiological Quality Assessment of Sashimi and Sushi Sold in the Federal District, Brazil. Journal of Food and Nutrition Research. 2020; 8(11):687-692. doi: 10.12691/jfnr-8-11-10

Abstract

This study aimed to evaluate the microbiological quality of sashimi and sushi sold in the Federal District, Brazil. A total of 54 samples (27 sashimi and 27 sushi) were collected in 25 commercial establishments. Samples were tested for counts of mesophilic and psychrotrophic bacteria, determination of total and thermotolerant coliforms, Staphylococcus aureus counts, and presence of Salmonella. The results showed that 40.7% of sashimi (11/27) and 25.9% of sushi (7/27) samples were unfit for consumption according to Brazilian legislation. Salmonella (genetically confirmed by the invA gene’s presence) was present in 25.9% of the sashimi samples (7/27). Thermotolerant coliforms were positive in 92.5% of sashimi samples (25/27) and 74.0% of sushi samples (20/27), and one sashimi sample was unfit for consumption (≥ 2.0 log MPN g^-1). S. aureus bacteria exceeded the acceptable limit of 3.0 log CFU g^-1 in 14.9% of sashimi samples (4/27) and 25.9% of sushi samples (7/27). A high count of S. aureus bacteria in some sushi and sashimi samples indicates improper personal hygiene while processing these ready-to-eat foods. Salmonella spp. can occur by improper handling of these foods or by the contact of fish with waters contaminated with sewage. These results can pose a health risk to consumers and indicated the need to improve good hygienic practices in establishments selling sashimi and sushi in Federal District, Brazil.

Keywords:
foodborne pathogens food hygiene Salmonella S. aureus ready-to-eat foods sashimi sushi

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References:

[1]	Lírio Rodrigues, S.P., Santos, D.F.C., Santos, M.A.O., Santos, W.I., Carvalho, M.G. “Avaliação da qualidade higiênicossanitária de restaurantes orientais (japonês e chinês) em Aracaju”. Revista Brasileira de Higiene e Sanidade Animal, 11(3), 289-306. 2017.
[2]	Braghini, F., Alexandrino, E.G., Leite, F.P., Kemmelmeier, E.G., Gonçalves, J.E. “Análise microbiológica de sashimis a base de salmão, comercializados na cidade de Maringá-PR.” Enciclopédia Biosfera, 11(22), 2015.
[3]	Rodrigues, B. L., Santos, L. R., Mársico, E. T., Camarinha, C. C., Mano, S. B., & Conte Junior, C. A. “Qualidade físico-química do pescado utilizado na elaboração de sushis e sashimis de atum e salmão comercializados no município do Rio de Janeiro, Brasil.” Semina: Ciências Agrárias, 33(5), 1847-1854. 2012.
[4]	Edwards, P. Global Sushi: Eating and Identity. Western Michigan University Publisher, Brill, 2012, 211-225.
[5]	Liang, L. W., Pan, Y. L., Cheng, H. L., Li, T. C., Yu, P. H. F., & Chan, S. W. “The microbiological quality of takeaway raw salmon finger sushi sold in Hong Kong.” Food Control, 69, 45-50. 2016.
[6]	Muscolino, D., Giarratana, F., Beninati, C., Tornambene, A., Pane Bianco, A., & Ziino, G. “Hygienic-sanitary evaluation of sushi and sashimi sold in Messina and Catania, Italy.” Italian Journal of Food Safety, 1701(3), 134-136 2014.
[7]	im, H. W., Hong, Y. J., Jo, J. I., Ha, S. D., Kim, S. H., Lee, H. J., & Rhee, M. S. “Raw ready-to-eat seafood safety: microbiological quality of the various seafood species available in fishery, hyper and online markets.” Letters in Applied Microbiology, 64, 27-34. 2016.
[8]	Souza, T. J. F. F., Silva, J. N., Silva Filho, C. R. M., & Santos, J. G. “Microrganismos de interesse sanitário em sushis.” Revista do Instituto Adolfo Lutz, 3(74), 274-279. 2015.
[9]	Hoel, S., Mehli, L., Bruheim, T., Vadstein, O., & Jakobsen, A. N. “Assessment of microbiological quality of retail fresh sushi from selected sources in Norway.” Journal of Food Protection, 78(5), 977-982. 2015.
[10]	Miguéis, S., Moura, A. T., Saraiva, C., & Esteves, A. “Influence of season and type of restaurants on sashimi microbiota.” The European Journal of Public Health, 26(5), 877-881. 2015.
[11]	Santos, M. I., Correia, C., Cunha, M. I. C., Saraiva, M. M., & Novais, M. R. “Valores-Guia para avaliação da qualidade microbiológica de alimentos prontos a comer preparados em estabelecimentos de restauração.” Revista da Ordem dos Farmacêuticos, 64, 66-68. 2005.
[12]	INSA, Instituto Nacional de Saúde Doutor Ricardo Jorge, Interpretação de resultados de ensaios microbiológicos em alimentos prontos para consumo e em superfícies do ambiente de preparação e distribuição alimentar: Valores-guia. INSA, Lisboa, 2019.
[13]	ICMSF, International Commission on Microbiological Specifications for Foods Microorganisms in foods 2. Sampling for microbiological analysis: principles and specific applications, International Commission on Microbiological Specifications for Foods Publisher, 1986.
[14]	Brasil. Agência Nacional de Vigilância Sanitária. Instrução normativa n° 60, de 23 de dezembro de 2019. Estabelece as listas de padrões microbiológicos para alimentos. Diário Oficial da União. 2019.
[15]	Lorentzen, G., Breiland, M. S. W., Cooper, M., & Herland, H. “Viability of Listeria monocytogenes in an experimental model of nigiri sushi of halibut (Hippoglossus hippoglossus) and salmon (Salmo salar).” Food Control, 25, 245-248. 2012.
[16]	Montanari, A. S., Romão, N. F., Sobral, F. O. S., Marmitt, B. G., Silva, F. P. S., & Correio, T. C. M. “Avaliação da qualidade microbiológica de sashimis de salmão, preparados e comercializados em restaurantes Japonês no município de Ji-Paraná - RO.” South American: Journal of Basic Education, Technical and Technological, 2(1), 4-16. 2015.
[17]	Santos, A. A., Simões, G. T. N., Cruz, M. M., Ferreira, N. S. S., Lima, R. T. C., & Tunon, G. I. L. “Avaliação da qualidade microbiológica de sushi comercializado em restaurantes de Aracaju, Sergipe.” Scientia Plena, 8(3), 1-5. 2012.
[18]	Valandro M. J., Campos, T., Paim, D., Cardoso, M., & Kindlein, L. “Avaliação da qualidade microbiológica de sashimis à base de salmão, preparados em restaurantes especializados em culinária japonesa.” Revista do Instituto Adolfo Lutz, 70(2), 144-150. 2011.
[19]	Leisner, J. J., Lund, T. B., Frandsen, E. A., Andersen, N. B. E., Fredslund, L., Nguyen, V. P. T., & Kristiansen, T. “What consumers expect from food control and what they get - a case study of the microbial quality of sushi bars in Denmark.” Food Control, 45, 76-80. 2014.
[20]	Kiedrowski, M. R., Kavanaugh, J. S., Malone, C. L., Mootz, J. M., Voyich, J. M., Smeltzer, M. S., Bayles, K. W., & Horswill, A. R. “Nuclease modulates biofilm formation in community-associated methicillin-resistant Staphylococcus aureus.” PLoS One, 6(11), 1-16. 2011.
[21]	Olson, M. E. et al. “Staphylococcus aureus nuclease is a SaeRS-dependent virulence factor.” Infection and Immunity. 81(4), 1316-1324. 2013.
[22]	Fernandes, D.V.G.S., Castro, V.S., Neto, A.C., Figueiredo, E.E.S. “Salmonella spp. in the fish production chain: a review.” Ciência Rural, 48(8), 1-11. 2018.
[23]	Novoslavskij, A., Terentjeva, M., Eizenberga, I., Valciņa, O., Bartkevičs, V., & Aivars Bērziņš, A. “Major foodborne pathogens in fish and fish products: a review.” Annals of Microbiology, 66, 1-15. 2016.
[24]	Malavota, L. C. M., Costa, J. C. B., Jardim, M. F., Oliveira, L. A. T., Franco, R. M., & Oliveira, V. M. “Ocorrência de Vibrio parahaemolyticus e Salmonella spp. em sashimis comercializados em restaurantes no município do Rio de Janeiro”, Revista Brasileira de Ciência Veterinária, 16(2), 89-94. 2009.
[25]	Karmi, M. “Detection of virulence gene (inva) in Salmonella isolated from meat and poultry products.” International Journal of Genetics, 3(2), 07-12. 2013.