Preservation Effects of Sourdough Bread Using Lacticaseibacillus Paracasei and Lactiplantibacillus Plantrum

Kotomi Iwabuchi¹, Jun Shima² and Noriko Komatsuzaki^1,

¹Department of Human Nutrition, Seitoku University, 550 Iwase Matsudo, Chiba, 271-8555, Japan

²Faculty of Agriculture, Ryukoku University, 1-5 Yokototani, Seta Oe-Cho, Ohtsu, Shiga, 520-2194, Japan

Cite this paper:
Kotomi Iwabuchi, Jun Shima and Noriko Komatsuzaki. Preservation Effects of Sourdough Bread Using Lacticaseibacillus Paracasei and Lactiplantibacillus Plantrum. American Journal of Food Science and Technology. 2023; 11(4):156-161. doi: 10.12691/ajfst-11-4-5

Abstract

The objective of this study was to compare the loaf quality and shelf life of sourdough and yeast-leavened. Sourdough bread is widely consumed in regions such as northern Europe and U.S while sourdough bread relatively unknown in Japan. Sourdough bread was made using novel lactic acid bacteria (LAB): Lacticaseibacillus paracasei NFRI 7415, isolated from traditional Japanese fermented fish, and Laciplantibacillus plantarum, an LAB which, together with L. paracasei, was recently shown to inhibit growth of Escherichia coli. A bread to which LAB were not added was made as a control. Although there were no significant differences in the weight, specific volume and water content among the three samples, the total sugar content of sourdough breads was lower than that of the control: co-fermentation of LAB and yeast appeared to increase sugar consumption. As expected, sourdough bread contained a higher content of organic acid, especially lactic acid. The sample loaves were cut into 1-cm slices and placed in a polypropylene bag, sealed, and stored at 28°C for 6 days. The general bacteria count of the control reached 10¹⁰ cfu/g in 6 days, while the sourdough bread was in the range of 10⁵–10⁶ cfu/g. The mold exposure tests showed mixed results, with the sourdough breads showing relatively suppressed growth of incidentally introduced mold, but not inoculated mold. These results indicate that the lactic acid produced by L. paracasei and L. plantarum was effective at inhibiting expansion of toxic bacteria but had no effect on mold to shelf.

Keywords:
Lacticaseibacillus sourdough sourdough bread organic acids preservation

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References:

[1]	Elmadfa, I., Klein, P., Meyer, A.L., Immune-stimulating effects of lactic acid bacteria in vivo and in vitro, Proc. Nutr. Soc., 69. 416-420. 2010.
[2]	Enomoto, T., Shimizu, K., Shimazu, S., Suppression of allergy development by habitual intake of fermented milk foods, evidence from an epidemiological study, Arerugi, 55. 1394-1399. 2006.
[3]	Ozaki, M., A new genealogy of lactic acid fermentation. Chuohoki Publishing, 206-224. 2004.
[4]	Nakada, H., Hasegawa, H., Sakurai, H., Tamura, M., Distinctive flavor and strong antifungal activity in a sourdough bread made using unique lactic acid bacteria obtained from a sugar factory, Nippon Shokuhin Kagaku Kogaku Kaishi, 57(2). 85-90. 2010. (in Japanese)
[5]	Reese, A.T., Madden, A.A., Joossens, M., Lacaze, G., Dunn, R.R., Influences of ingredients and bakers on the bacteria and fungi in sourdough starters and bread. American Soci. Microbiol., 5(1). e00950-19. 2020.
[6]	Gobbetti, M., The sourdough microflora: interactions of lactic acid bacteria and yeasts. Trends Food Sci. Technol., 9(7). 267–274. 1998.
[7]	Axel, C., Zannini, E., Arendt, E.K., Mold spoilage of bread and its biopreservation: A review of current strategies for bread shelf life extension, Crit. Rev. Food Sci. Nutr., 57(16). 3528-3542. 2017.
[8]	Komatsuzaki, N., Shima, J., Kawamoto, H., Momose, H., Production of -aminobutyric acid (GABA) by Lactobacillus paracasei isolated from traditional fermented foods, Food. Microbiol., 22(6). 497-504. 2005.
[9]	Komatsuzaki, N., Nakamura, T., Kimura, T., Shima, J., Characterization of glutamate decarboxylase from a high-aminobutyric acid (GABA)-producer, Lactobacillus paracasei, Biosci. Biotechnol. Biochem., 72. 278-285. 2008.
[10]	Iwabuchi, K., Shima, J., Komatsuzaki, N., Antibacterial effect of lactic acid bacteria on the cariogenic bacteria Streptococcus mutans, J. Integr. Stud. Diet. Habit., 32(4). 171-176. 2022. (In Japanese)
[11]	Kudou, H, Amino, C., Hoshio, N., Shima, J., Komatsuzaki, N., Simulated digestive juice tolerance and effect on cecal organic acods of mice of Lactobacillus paracasei NFRI 7415, J. Integr. Stud. Diet. Habit., 31(3). 131-138. 2020.
[12]	Nishida, O., Kuwazaki, S., Suzuki, C., Shima, J., Superior molasses assimilation, stress tolerance, and trehalose accumulation of baker’s yeast isolated from dried sweet potatoes (hoshi-imo). Biosci. Biotechnol. Biochem., 68, 1442-1448. 2004.
[13]	Yue, F., Zhang, J., Xu, J., Niu, T., Lu, X., Liu, M., Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method, Frontiers in Nutrition.
[14]	Gul, H., Ozcelik, S., Sagdic, O., Certel, M., Sourdough bread production with lactobacilli and S. cerevisiae isolated from sourdoughs, Process Biochemistry, 40. 691-697. 2005.
[15]	Sato, R., Shima, J., Suzuki, C., Momose, H., Kawamoto, S., Characteristic evaluation of lactic acid bacteria collections based on the bacteriocin productivity, Rep. Natl. Food. Res. Inst., 66. 15-20. 2002.
[16]	Axel, C., Röcker, B., Brosnan, B., Zannini, E., Furey, A., Coffey, A., Arendt, E.K., Application of Lactobacillus amylovorus DSM19280 in gluten-free sourdough bread to improve the microbial shelf life. Food. Microbiol., 47. 36–44. 2015.
[17]	Ryan, L.A.M., Zannini, E., Dal Bello, F., Pawlowska, A., Koehler, P., Arendt, E.K., Lactobacillus amylovorus DSM 19280 as a novel food-grade antifungal agent for bakery products, Int. J. Food. Microbiol., 146(3). 276–283. 2011